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Register a new userأمثلة ظروف إنتاج الكتلة الحيوية لخميرة الخباز باستخدام بيئة مستخلص التمور والمولاس
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Damascus University رسالة ماجستير
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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Shamra Editor
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جمعت 30 عينة عشوائية من مواد غذائية مختلفة من محافظتي دمشق وحمص لعزل وتشخيص الخمائر مورفولوجياً حسب Barnett وفيزيولوجياً باستخدام تقنية API
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وزارة الزراعة والاصلاح الزراعي 2012
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Ten randomized samples of materials were collected from two
places (Damascus, Homs)in Syria for isolation and identification
yeasts. These yeasts were classified morphologically according to
Barnett's and physiologically by API technique .Results
showed
that 6 isolated yeasts were Saccharomyces cerevisiae . These
isolated yeasts were screened to investigate production of biomass.
The results showed that there were differences in the ability of
isolated yeasts to produce biomass. Saccharomyces cerevisiae
isolated from date fruit was best yeast for biomass production.
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
This study aimed to identify the most important factors affecting dates
production, in Wadi al Shati, Libya by estimating indicators of economic
efficiency of the resources used to measure the impact of each of the
components used in date producti
on. The field study indicated that the most
important productive inputs affecting significantly crop production (dates)
were number of trees, the quantity of fertilizer, water quantity and the number
of employment and expertise at different moral levlss. This has proven to be a
significant variables at different moral levels. The results also showed that the
elasticities of Wadi al Shati villages and the beach were positive and operating
in the first phase of increased production to reach about 0.847 and reflect the
return of diminishing capacity.
The study was conducted in 2016. The samples of rice Oryza sativa L. straw
were collected from Dewania governorate/Iraq, and were brought to laboratory of
Directorate of Environmental and Water in Ministry of Science and Technology.
The samples we
re cleaned and milled, then stored in sterile containers. Local
cellulolytic bacterial isolate was cultivated and isolated on mineral and cellulose
medium, at 37 ºC for (24± 2) hours, the bacterial isolate was diagnosed as Bacillus sp.
depending on phenotypes of bacterial colonies on solid medium, microscopic
characters and some biochemical tests. Milled rice straw was chemically treated
with 1% of sodium hydroxide, then biological treatment by bacterial isolate Bacillus sp
cultivate in mineral medium with alkali treated rice straw as carbone source and
compared with cellulose standard medium. The bacterial growth was measured at
600 nm, which reached 0.974 in rice straw medium, while in cellulose medium
reached 0.853. For glucose concentration, the value reached 250 μg/ml in rice straw
medium, while in cellulose medium it was 210 μg/ml. The results concluded the
possibility of getting rid of rice husks, which is an environmental contaminant, and
to use it in the production of glucose.
45 samples were collected from various sources (soil, degraded wood and
mushroom compost) from three cities (Damascus, Homs and Lattakia). 18
Trichoderma isolates were isolated and identified by microscope. These isolates
were screened using CMC m
edium with Congo red dy to identify their ability
to produce cellulase complex. The amount of enzyme production was
determined depending on the radius of clear zone around the colony. Results
showed that the most productive isolate was Tr with radius 7 ± 0.2 cm followed
by Tg and Ti. Optimization of cellulase production was performed using
response surface methodology (RSM). The optimal parameters were:
temperature 29.5 ˚C, pH = 6، incubation time 4 days, aeration speed 175 rpm
and wheat straw concentration 3%. All studied parameters had significant
effect on cellulase enzyme activity.
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