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Register a new userOptimization of bioethanol production using Sugar Sorghum Stem juice
أمثلة إنتاج الإيثانول الحيوي من عصارة سوق الذرة البيضاء السكرية
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Aِl-Baath University ورقة بحثية
Publication date
2016
fields
Technical Architecture
and research's language is
العربية
Authors
رحيم ابو الجدايل( باحث )
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تعد تقانة إنتاج الوقود الحيوي السائل بتخمير المخلفات النباتية من الأساليب المتقدمة تكنولوجياً للاستفادة من مخلفات الحاصلات الزراعية في الحصول على طاقة صديقة للبيئة. هدف البحث إلى دراسة استثمار عصارة نبات الذرة البيضاء السكرية في إنتاج الإيثانول الحيوي بطريقة الدفعة Batch و باستخدام خميرة cerevisiae Saccharomyces.
Producing of biofuel from crops wastes is considered one of the advanced eco- friendly technologies. This research was conducted in the General Commission For Biotechnology in order to study the possibility to use the juice of sugar sorghum plant as alternative substrate to produce bioethanol using S. cerevisiae and optimize the production’ conditions.
References used
AOAC International (2007) Official Methods of Analysis, 18th edn, 2005; Current through revision 2, 2007 (On-line). AOAC International, Gaithersburg, M.D
JIRU, T.M. 2009. Evaluation of yeast biomass production using molasses and supplements, Master thesis, Addis Ababa University, Faculty of Science. P70
KIM, K.J., D. Yoshiharu and A. Hideki. 2006. Effects of residual metal compounds and chain-end structure on thermal degradation of poly (3-hydroxybutyric acid), Polymer Degradation and Stability, 1(91). 769 -777
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The research aimed to study the possibility of using sweet sorghum
juice as a substrate to produce Baker’s yeast and optimizing the
temperature and agitation rate to obtain the highest biomass yield by
batch method in beaker flasks then in laborat
ory fermenter.
This study was conducted at the laboratories of the Faculty of Agriculture,
Damascus University and the National Commission of Biotechnology in 2012 to
investigate he possibility of Production of bioethanol using local isolate of
Saccharomyces cer
evisiae from raw sugar refining molasses and to determine
the effect of different temperatures, pH and concentration of Dry matter (Brix
degree) in molasses on the amount of ethanol produced. A local isolate (R1) of
Saccharomyces cerevisiae selected previously due to its superiority in ethanol
production was taken from wine brewing residues and exposed to different
temperatures (20, 25, 30 and 35OC), pH (4, 4.5, 5 and 5.5) and the Brix degrees
(16, 20, 24 and 28%) during fermentation.
Results revealed that the best amount of ethanol was produced at
temperature of 30 º C, pH 5, and Brix degree in molasses 16%.
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
45 samples were collected from various sources (soil, degraded wood and
mushroom compost) from three cities (Damascus, Homs and Lattakia). 18
Trichoderma isolates were isolated and identified by microscope. These isolates
were screened using CMC m
edium with Congo red dy to identify their ability
to produce cellulase complex. The amount of enzyme production was
determined depending on the radius of clear zone around the colony. Results
showed that the most productive isolate was Tr with radius 7 ± 0.2 cm followed
by Tg and Ti. Optimization of cellulase production was performed using
response surface methodology (RSM). The optimal parameters were:
temperature 29.5 ˚C, pH = 6، incubation time 4 days, aeration speed 175 rpm
and wheat straw concentration 3%. All studied parameters had significant
effect on cellulase enzyme activity.
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