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Register a new userEffect some conditions culture on growth Range chlorella vulgaris in laboratory
تأثير بعض ظروف الاستزراع على معدل نمو الطحلب الأخضر Chlorella vulgaris مخبرياً
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تناولت هذه الدراسة عزل طحلب Chlorella vulgaris من بعض المسطحات المائية و اختبار تأثير بعض الشروط (شدة الإضاءة – تركيز النترات و الفوسفات و محلول التربة) على نموه و زمن تضاعف خلاياه.
In this study we isolated chlorella vulgaris alage from some water areas , and test effect some conditions (light intensity,Nitrat and phosphate concentrations ,solution soil) on its Growth Range and Duplicated time the cells.
References used
AHMED, M A, DAWWOOD, S , ANFAL, N A, 2012 - The quantity determination of total carbohydrates and monosaccharides from some green algae (Chlorophyta). Marsh Bulletin, 27-38p
AL-AARAGY,MJ,1996 -Studies on the mass culture of freshwater microalgae as live food for fish larvae.Ph.D.thesis,Collegeof Science, Basrah Uviversity., 107pp
BARBOSA,JM,HOOGAKKER,J,WIJFFELS,HR,2003 Optimisation of cultivation parameters in photobioreactors for microalgae cultivation using the A-stat technique, Biomolecular Engineering , 115-123p
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The effect of different concentrations of phosphate and nitrate and soil extracted on
the isolated growth isolation of Scendesmus acutus which is isolated from some local
aquatic areas has tested in this research, the number of cells and growth ran
ge and
duplicated time of all algal cultures among thirty five days has counted.
The highest growth range was 3.90 by using a mixture of the three solutions in
concentrations (1g/l nitrate + 0.05g/l phosphate + 10 ml / 100 of soil solution), the numbers
of cells reached to 8861.11 in the twenty fourth day of culture, and in the lowest duplicated
time 10.85, whereas the highest growth range was by using soil solution alone 2.86 and the
lowest duplicated time 2.13 at concentration 16/100ml, whereas the growth range
decreased and duplicated time increased by using nitrate and phosphate solution all by its
own, while the highest growth range reached to 2.13 and 1.76 and lowest duplicated time
3.39 and 4.11 in order.
The effect of different concentrations of nitrate and phosphate and soil extract on the
total growth and chemical composition to the green algae Chorella vulgaris isolated from
some localaquatic habitats had studied in this research, at three type
of temperature (30 ,
25, 20), the maximum total growth reached up to 4.66 Cell/h and the lowest duplicated
time was 1.55 h at temperature 25ºC and at concentration of nitrate 1 g/L , 0.05 g/L of
phosphate, and 10/100 mL of soil solution , while the number of cells reached to 59423,61
cell/mL at the day 22 of cultivation.
The chemical composition differed by the changing of temperature and nutrient
medium composition , while the maximum protein content reached to 48.36 % at the
maximum concentration of nutrients and 25 ºC of temperature , the maximal concentration
of lipid was 19,78 % at temperature 25ºC and concentration of nitrate 0.1 g/L and 0.01 of
phosphate and 6/100 mL of soil solution , the concentration of carbohydrates were the
lowest where the maximal ratio was 17.41 % at temperature 20ºC .
According to the algae ability in photosynthesis and carbohydrate production with
high value in nutrition and industry, this research was carried out during 2015 to study the
qualitative and quantitative content of carbohydrates in Chlorella vulgar
is which was
isolated from southern Syrian fresh water, and to determine the effect of the growth
conditions; temperature (15, 25, 35 C), sodium nitrate concentration (0.1, 0.25, 0.4 g/l) and
pH (6, 7, 8) on that content. The results showed that the total content was in the range of
20% to 40%;with galactose and rhamnose dominating among the rest of sugars. The
temperature 15°C was the best to get the greatest total content with the highest value of
starch, cellulose, hemicellulose and pectin. In addition, the low nitrate concentration 0.1 g/l
was combined with the highest content of pectin and its compounds, while the glucose
content was not affected by the nitrogen concentration. Related to pH effect, the acidic
media was proved to be the best for the highest carbohydrate content. Moreover, cellulose,
starch, and pectin increased in the alkali medium over the rest of carbohydrates.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
The experiment was carried out during spring season 2016 to study the effect
of foliar spraying treatment with Tecamin Flower 2.5 ml/L in comparison with
distilled water, on growth of three hybrids of tomatoes at two different levels of
irrigation
(100 and 50 % of field capacity). Three sprays were conducted started
from flowering with two weeks’ interval. The results showed that ‘Finenss’ hybrid
had significant differences in plant height, branches per plant and leaves number per
plant. ‘Hadeer’ hybrid had superiority in dry matter ratio and content of chlorophyll
in leaves. Reducing the level of irrigation resulted in a significant decrease in
plant height, total branches number, versus an increase in the leaves number per
plant, total leaves area and the percentage of dry matter. The results showed that
the treatment with Tecamin flower led to a significant increase in all indicators
studied. The interaction treatments shoed that Hadeer hybrid irrigated in level 50
% and spraying of nutrient solution (Tecamin flower) had a significant increase in
dry matter ratio and chlorophyll content in leaves. While the hybrid Finenss which
irrigated in level 100 % with nutrient solution (Tecamin flower) had a significant
increase in leaves number per plant and total leaves area.
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