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Effect of some growth conditions on thequantity and quality of carbohydrates in Chlorella vulgarisisolated from fresh water in southern Syrian

تأثير بعض ظروف النمو على كمية الكربوهيدرات و نوعيتها في طحلب Chlorella vulgaris المعزول من المياه العذبة في جنوب سورية

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 Publication date 2017
and research's language is العربية
 Created by Shamra Editor




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According to the algae ability in photosynthesis and carbohydrate production with high value in nutrition and industry, this research was carried out during 2015 to study the qualitative and quantitative content of carbohydrates in Chlorella vulgaris which was isolated from southern Syrian fresh water, and to determine the effect of the growth conditions; temperature (15, 25, 35 C), sodium nitrate concentration (0.1, 0.25, 0.4 g/l) and pH (6, 7, 8) on that content. The results showed that the total content was in the range of 20% to 40%;with galactose and rhamnose dominating among the rest of sugars. The temperature 15°C was the best to get the greatest total content with the highest value of starch, cellulose, hemicellulose and pectin. In addition, the low nitrate concentration 0.1 g/l was combined with the highest content of pectin and its compounds, while the glucose content was not affected by the nitrogen concentration. Related to pH effect, the acidic media was proved to be the best for the highest carbohydrate content. Moreover, cellulose, starch, and pectin increased in the alkali medium over the rest of carbohydrates.


Artificial intelligence review:
Research summary
تتناول هذه الدراسة تأثير بعض ظروف النمو على كمية ونوعية الكربوهيدرات في طحلب Chlorella vulgaris المعزول من المياه العذبة في جنوب سوريا. تم دراسة تأثير درجة الحرارة (15، 25، 35 درجة مئوية)، تركيز نترات الصوديوم (0.1، 0.25، 0.4 غ/ل)، وقيم pH (6، 7، 8) على محتوى الكربوهيدرات. أظهرت النتائج أن كمية الكربوهيدرات الكلية تراوحت بين 20% و40%، مع سيادة الغالاكتوز والرامنوز على باقي السكريات. كانت درجة الحرارة 15 درجة مئوية هي الأفضل للحصول على أكبر كمية من الكربوهيدرات بأعلى نسبة من النشا والسليلوز والهيميسليلوز والبكتين. كما أن التركيز المنخفض (0.1 غ/ل) من نترات الصوديوم أعطى أكبر كمية من الكربوهيدرات بأعلى محتوى من البكتين. وأظهرت الدراسة أن الوسط الحمضي هو الأفضل للحصول على أكبر كمية من الكربوهيدرات، بينما ازدادت نسبة السليلوز والنشا والبكتين في الوسط القلوي على حساب باقي المركبات. توصي الدراسة بزيادة الاهتمام بالأبحاث التطبيقية على الطحالب الخضراء الدقيقة المنتشرة في المياه السورية لأهميتها كمصدر متجدد للكتلة الحيوية.
Critical review
دراسة نقدية: تقدم هذه الدراسة معلومات قيمة حول تأثير ظروف النمو المختلفة على محتوى الكربوهيدرات في طحلب Chlorella vulgaris. ومع ذلك، يمكن توجيه بعض النقد البناء لتحسين الدراسة. أولاً، كان من الممكن أن تشمل الدراسة مجموعة أوسع من الظروف البيئية مثل تأثير الضوء ونسبة ثاني أكسيد الكربون، مما قد يعطي صورة أشمل عن تأثير هذه العوامل على محتوى الكربوهيدرات. ثانياً، لم تتناول الدراسة تأثير هذه الظروف على الجوانب الأخرى من الطحلب مثل إنتاج البروتينات أو الليبيدات، والتي قد تكون ذات أهمية كبيرة في التطبيقات الصناعية. أخيراً، كان من الممكن أن تكون الدراسة أكثر شمولية إذا تضمنت مقارنة مع أنواع أخرى من الطحالب الخضراء الدقيقة لتحديد ما إذا كانت النتائج خاصة بـ Chlorella vulgaris أم أنها تنطبق على أنواع أخرى أيضاً.
Questions related to the research
  1. ما هي درجة الحرارة المثلى للحصول على أكبر كمية من الكربوهيدرات في طحلب Chlorella vulgaris؟

    درجة الحرارة المثلى هي 15 درجة مئوية.

  2. ما هو تأثير تركيز نترات الصوديوم على محتوى الكربوهيدرات في الطحلب؟

    التركيز المنخفض (0.1 غ/ل) من نترات الصوديوم أعطى أكبر كمية من الكربوهيدرات بأعلى محتوى من البكتين.

  3. ما هي السكريات الأحادية السائدة في طحلب Chlorella vulgaris؟

    السكريات الأحادية السائدة هي الغالاكتوز والرامنوز.

  4. كيف يؤثر الوسط الحمضي على محتوى الكربوهيدرات في الطحلب؟

    الوسط الحمضي هو الأفضل للحصول على أكبر كمية من الكربوهيدرات الكلية.


References used
APHA, AWWA, WPCF. Standards methods for the examination of water and wastewater. 21st ed., APHA-AWWA-WPCF, Washington, DC, 2005
ATHBI, A. M., Dawwood S. A. and Anfal N. A. The quantity determination of total carbohydrates and monosaccharides from some green algae (ChloropHyta). Marsh Bulletin, Vol. 7, N°.1, 2012, 27-38
BATTAH, M. G., El-Ayoty, Y. M. Esmael, A. E. and Abd El-Ghany, S. E. Effect of different concentrations of sodium nitrate, sodium chloride, and ferrous sulpHate on the growth and lipid content of Chlorella vulgaris. Journal of Agricultural Technology, Vol. 10, N°. 2, 2014, 339-353
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