Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userExtraction of carotenoids from Rhodotorula yeast isolated from local sources
ستخلاص أشباه الكاروتينات من خميرة Rhodotorula المعزولة من مصادر محلية
1680
0 44
0
(
0
)
Added by
Damascus University ورقة بحثية
Publication date
2014
fields
Food Sciences
and research's language is
العربية
Created by
Shamra Editor
Ask ChatGPT about the research
جمعت 50 عزلة خميرة من الجنس Rhodotorula من مصادر محلية متنوعة خلال عامي 2012 و 2013 ،منها 13 عزلة من التربة و 23 من أوراق الأشجار و 14 من مواد غذائية. شخـصت العـزلات باسـتخدام تقانـة AUX 20c API و تبـين أن العزلات تنتمـي إلــى ثلاثـة أنواع هــي على التـوالي علـى %، 4 و % 20 و % 76 و بنـسب R. minuta و ، R. glutinis و ، R. mucilaginosa استخدمت ست طرائق مختلفة لتحطيم خلايا الخميرة و استخلاص الصبغة و أظهرت النتائج فيمـا يخـص كمية استخلاص أشباه الكاروتينات تفوق الطريقة المعدلة باستخدام DMSO و التحضين لمدة 24 سـاعة مع التحريك عند 4°س على الطرائق الخمس الأخرى المستخدمة كافة. تميزت جميع العزلات بقدرتها على إنتاج أشباه الكاروتينات بكفاءات مختلفة حيث تفوقت العزلة A24 mucilaginosa. R المعزولة مـن الغذاء بكمية أشباه الكاروتينات المنتجة إذ بلغت 23.658 ميكروغرام/غ كتلة حيوية جافة للخلايا مقارنـة ببقية العزلات الأخرى.
Rhodotorula yeast was isolated from various local sources. Fifty isolates were collected during the years 2012-2013 where 13 were isolated from soil, 23 from tree leaves and 14 from food. The isolates AUX system. Were classified into three Rhodotorula species: R. mucilaginosa, R. glutinis and R. minuta with 76%, 20% and 4% respectively using API 20c. Six methods were applied in breaking down the yeast cells and extracting the carotenoids. It was shown the quantity of carotenoides extracted with the modified method by adding DMSO to the yeast biomass and incubating for 24 hrs at 4˚C yielded in higher quantities when compared with the other five methods. All isolates were able to produce carotenoides but they varied in their efficiency where the isolate A24 (R.mucilaginosa) isolated from food was distinguished by its high level of production which reached 658.23 μg/g dry weight compared with the others.
References used
Aksu, Z. and A. T. Eren. 2005. Carotenoids production by the yeast Rhodotorula mucilaginosa: Use of agricultural wastes as a carbon source. Process Biochemistry .40:2985–2991
Astorg, P. 1997. Food carotenoids and cancer prevention. An overview of current research. Trends Food Sci. Technol. 8:406-413
Bendich, A. 1994. Recent advances in clinical research involving carotenoids. Pure Appl. Chem. 66: 1017-1024
rate research
Read More
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
This study was conducted at the laboratories of the Faculty of Agriculture,
Damascus University and the National Commission of Biotechnology in 2012 to
investigate he possibility of Production of bioethanol using local isolate of
Saccharomyces cer
evisiae from raw sugar refining molasses and to determine
the effect of different temperatures, pH and concentration of Dry matter (Brix
degree) in molasses on the amount of ethanol produced. A local isolate (R1) of
Saccharomyces cerevisiae selected previously due to its superiority in ethanol
production was taken from wine brewing residues and exposed to different
temperatures (20, 25, 30 and 35OC), pH (4, 4.5, 5 and 5.5) and the Brix degrees
(16, 20, 24 and 28%) during fermentation.
Results revealed that the best amount of ethanol was produced at
temperature of 30 º C, pH 5, and Brix degree in molasses 16%.
Thirty randomized samples of sugar materials were collected from different
places (Tartous, Damascus, Homs) in Syria for isolation and identification of
some yeasts. These yeasts were classified physiologically by API technique
to identify the spe
cies of Yeasts found in these materials. Results showed that
the isolated materials contained 17 isolated yeasts distributed in 3 genus and 7
species and named as follows: One isolated yeast from Geotrichum capitatum,
Candida krusei,Candida guilliermondi, Candida fumata and Candida zeylanoides
and 4 isolated yeasts from Candida lusitaniae and 7 isolated yeasts from
Saccharomyces cerevisiae. The classified yeasts were screened to investigate the
activity of internal and external invertase produced by them using
spectrophotometer at 540 nm. The results showed that there were differences in
the ability of yeasts to produce invertase enzyme. Candida Lusitaniae-2 and
Saccharomyces cerevisiae-4 were found the best yeasts for production of
external invertase, whereas Candida Lusitaniae1 was the best for internal
invertase.
This research was conducted estimate total count of bacteria, and the census total coliform group, fecal coliforms, Escherichia coli .
Sixty yeasts spp. were isolated from samples of whey which collected from
different locations in Syria. These yeasts were classified morphologically
according to Barnett’s and physiologically by API technique to identify the
species of Yeasts, whe
re had found 10 classified yeasts as follows:
Candida krusei is the most of yeasts which founded in whey with 21.66 %,
and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came
(Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with
11.66%, after that the Candida magnolia is ranked in the fourth rank by
distributing with 8.33%. as soon as the Candida famata came in the fifth rank
with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%,
finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank
with 3.33%, so we noticed the variety of the yeasts in the studied locations in
Syria.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
