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Register a new userMeasuring the effectiveness of invertase activity in isolated yeasts from local sugary materials
قياس فعالية الإنفرتاز في عزلات خمائر من مواد سكرية محلية مختلفة
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جمعت 30 عينة من مواد سكرية من محافظات سورية (طرطوس، و دمشق، و حمص) مختلفة لعـزل الخمائر فيزيولوجياً و تشخيصها باستخدام تقنية ال API ، أظهرت النتائج وجود 17 عزلة توزعت علـى 3 أجناس و 7 أنواع من الخمائر صنفت على الشكل الآتي: عزلـة واحـدة مـن كـل مـن Geotrichum عزلات و أربع C. guilliermondi ،C. fumata ،C.zeylanoides ،Candida krusei ،capitatum من lusitaniae. C و سبعة من cerevisiae Saccharomyces . غُربلتْ هذه الخمائر المصنّفة بحـسب نشاط الإنفرتاز الداخلي و الخارجي الناتج عن كل منها باستخدام جهاز الطيفي الضوئي عند طـول موجـة 540nm .بينت النتائج تباين السلالات المدروسة في فعالية الإنفرتاز الناتج عنها إذْ كان النشاط الإنزيمي للأنفرتاز الخارجي الناتج عن السلالتين 2-Lusitaniae. C و 4 -cerevisiae Saccharomyces الأعلـى في حين كان الإنفرتاز الداخلي للسلالة Lusitaniae1. C الأكثر نشاطاً.
Thirty randomized samples of sugar materials were collected from different places (Tartous, Damascus, Homs) in Syria for isolation and identification of some yeasts. These yeasts were classified physiologically by API technique to identify the species of Yeasts found in these materials. Results showed that the isolated materials contained 17 isolated yeasts distributed in 3 genus and 7 species and named as follows: One isolated yeast from Geotrichum capitatum, Candida krusei,Candida guilliermondi, Candida fumata and Candida zeylanoides and 4 isolated yeasts from Candida lusitaniae and 7 isolated yeasts from Saccharomyces cerevisiae. The classified yeasts were screened to investigate the activity of internal and external invertase produced by them using spectrophotometer at 540 nm. The results showed that there were differences in the ability of yeasts to produce invertase enzyme. Candida Lusitaniae-2 and Saccharomyces cerevisiae-4 were found the best yeasts for production of external invertase, whereas Candida Lusitaniae1 was the best for internal invertase.
References used
Aehle, W. 2007. Enzymes in industry, production and applications, Third, Completely Revised Edition, WILEY-VCH Verlag GmbH & Co. KgaA, Netherland, Pp: 4-25
Belcarz, A., G. Ginalska, J. Lobarsewski and C. Penel. 2002. The novel nonglycosylated invertase from Candida utilis the properties and the conditions of production and purification, Biochim. Biophys. Acta, 1594: 40–53
Ikram, H., A. Mirza and A. Sikander. 2005. Effect of cultivation conditions on invertase production hyper producing Saccharomyces cerevisiae isolates, W. J. Microbio. and Biotechno., 21:487–492
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