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Register a new userMeasuring the effectiveness of invertase activity in isolated yeasts from local sugary materials
قياس فعالية الإنفرتاز في عزلات خمائر من مواد سكرية محلية مختلفة
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جمعت 30 عينة من مواد سكرية من محافظات سورية (طرطوس، و دمشق، و حمص) مختلفة لعـزل الخمائر فيزيولوجياً و تشخيصها باستخدام تقنية ال API ، أظهرت النتائج وجود 17 عزلة توزعت علـى 3 أجناس و 7 أنواع من الخمائر صنفت على الشكل الآتي: عزلـة واحـدة مـن كـل مـن Geotrichum عزلات و أربع C. guilliermondi ،C. fumata ،C.zeylanoides ،Candida krusei ،capitatum من lusitaniae. C و سبعة من cerevisiae Saccharomyces . غُربلتْ هذه الخمائر المصنّفة بحـسب نشاط الإنفرتاز الداخلي و الخارجي الناتج عن كل منها باستخدام جهاز الطيفي الضوئي عند طـول موجـة 540nm .بينت النتائج تباين السلالات المدروسة في فعالية الإنفرتاز الناتج عنها إذْ كان النشاط الإنزيمي للأنفرتاز الخارجي الناتج عن السلالتين 2-Lusitaniae. C و 4 -cerevisiae Saccharomyces الأعلـى في حين كان الإنفرتاز الداخلي للسلالة Lusitaniae1. C الأكثر نشاطاً.
Thirty randomized samples of sugar materials were collected from different places (Tartous, Damascus, Homs) in Syria for isolation and identification of some yeasts. These yeasts were classified physiologically by API technique to identify the species of Yeasts found in these materials. Results showed that the isolated materials contained 17 isolated yeasts distributed in 3 genus and 7 species and named as follows: One isolated yeast from Geotrichum capitatum, Candida krusei,Candida guilliermondi, Candida fumata and Candida zeylanoides and 4 isolated yeasts from Candida lusitaniae and 7 isolated yeasts from Saccharomyces cerevisiae. The classified yeasts were screened to investigate the activity of internal and external invertase produced by them using spectrophotometer at 540 nm. The results showed that there were differences in the ability of yeasts to produce invertase enzyme. Candida Lusitaniae-2 and Saccharomyces cerevisiae-4 were found the best yeasts for production of external invertase, whereas Candida Lusitaniae1 was the best for internal invertase.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة قياس فعالية إنزيم الإنفرتاز في عزلات الخمائر المستخرجة من مواد سكرية محلية في سوريا. تم جمع 30 عينة من محافظات طرطوس، دمشق، وحمص، وتم عزل وتشخيص الخمائر باستخدام تقنية الـ API. أظهرت النتائج وجود 17 عزلة من الخمائر موزعة على 3 أجناس و7 أنواع. تم فحص نشاط الإنفرتاز الداخلي والخارجي باستخدام جهاز الطيفي الضوئي عند طول موجة 540 نانومتر. أظهرت النتائج تباين السلالات في قدرتها على إنتاج الإنفرتاز، حيث كانت السلالات C. Lusitaniae-2 وSaccharomyces cerevisiae-4 الأكثر فعالية في إنتاج الإنفرتاز الخارجي، بينما كانت السلالة C. Lusitaniae-1 الأكثر فعالية في إنتاج الإنفرتاز الداخلي. توصي الدراسة بضرورة دراسة شروط إفراز الإنفرتاز المثالية من السلالات الأكثر إنتاجاً واستخدامه في الصناعات الغذائية والدوائية.
Critical review
دراسة نقدية: تعتبر هذه الدراسة ذات قيمة علمية عالية حيث تسلط الضوء على إمكانيات الخمائر المحلية في إنتاج إنزيم الإنفرتاز، وهو إنزيم ذو أهمية كبيرة في الصناعات الغذائية والدوائية. ومع ذلك، يمكن توجيه بعض النقد البنّاء للدراسة، مثل الحاجة إلى توسيع نطاق العينات لتشمل مناطق جغرافية أخرى في سوريا لضمان تمثيل أوسع للخمائر المحلية. كما يمكن تحسين منهجية الدراسة من خلال استخدام تقنيات تحليلية أكثر تطوراً لقياس فعالية الإنزيمات بدقة أكبر. بالإضافة إلى ذلك، يمكن أن تكون الدراسة أكثر شمولية إذا تضمنت تحليلاً اقتصادياً لتكلفة إنتاج الإنفرتاز من الخمائر المحلية مقارنة بالطرق التقليدية.
Questions related to the research
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ما هي المناطق التي تم جمع العينات منها في هذه الدراسة؟
تم جمع العينات من محافظات طرطوس، دمشق، وحمص في سوريا.
-
ما هي التقنية المستخدمة لتشخيص الخمائر في هذه الدراسة؟
تم استخدام تقنية الـ API لتشخيص الخمائر.
-
أي السلالات كانت الأكثر فعالية في إنتاج الإنفرتاز الخارجي؟
كانت السلالات C. Lusitaniae-2 وSaccharomyces cerevisiae-4 الأكثر فعالية في إنتاج الإنفرتاز الخارجي.
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ما هي التوصيات التي قدمتها الدراسة بشأن استخدام الإنفرتاز؟
توصي الدراسة بضرورة دراسة شروط إفراز الإنفرتاز المثالية من السلالات الأكثر إنتاجاً واستخدامه في الصناعات الغذائية والدوائية.
References used
Aehle, W. 2007. Enzymes in industry, production and applications, Third, Completely Revised Edition, WILEY-VCH Verlag GmbH & Co. KgaA, Netherland, Pp: 4-25
Belcarz, A., G. Ginalska, J. Lobarsewski and C. Penel. 2002. The novel nonglycosylated invertase from Candida utilis the properties and the conditions of production and purification, Biochim. Biophys. Acta, 1594: 40–53
Ikram, H., A. Mirza and A. Sikander. 2005. Effect of cultivation conditions on invertase production hyper producing Saccharomyces cerevisiae isolates, W. J. Microbio. and Biotechno., 21:487–492
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