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Register a new userIsolation of Streptomyces sp. with antibacterial activity against some human pathogenic bacteria from soils of Lattakia city
عزل جرثوم المتسلسلة Streptomyces sp يمتلك خصائص مضادة لبعض الجراثيم الممرضة البشرية من ترب مدينة اللاذقية
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Aِl-Baath University ورقة بحثية
Publication date
2016
fields
Biology
and research's language is
العربية
Authors
أسمهان زينب( باحث )
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تم الحصول على أربع عزلات لجراثيم Streptomyces sp و هي : St.1 و St.2 من تربة حديقة كلية العلوم, و St.3 و St.4 من تربة منطقة الشامية, بطريقة التخفيف على وسط Starch Casein Agarّ.
Four isolates of Streptomyces sp., St.1 and St.2 were isolated from garden soil of science faculty, St.3 and St.4 were isolated from Al-Shameia soil Lattakia, by serial dilution on Starch Casein Agar medium.
References used
ANANSIRIWATTANA W.; TANASUPAWAT S.; AMNUOYPOL S. and SUWANBORIRUX K. 2006, Identification and antimicrobial activities of actinomycetes from soils in Samed Island, and geldanamycin from strain PC4 – 3. Thia. J. Pharm. Sci., Vol. 30, pp. 49-56
ARAI, T. 1976, Actinomycetes, the boundary Microorganisms. Toppan, Tokyo, 123p
ARIFUZZAMAN M.; KHATUN M. R. and RAHMAN H. 2010, Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity. African Journal of Biotechnology, Vol. 9, No. 29, pp. 4615-4619
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The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
Pyocyanin was extracted from cultures of Pseudomonas
aeruginosa, which was isolated from sea water by chloroform.
Results study the effect of pyocyanin against human pathogenic
bacteria, which were isolated from Al-Assad hospital laboratory in
La
ttakia, with disk diffusion method showed that pyocyanin has
antibacterial activity against Gram- positive bacteria.
Inhibition activity of extracts (acetone, ethanol, water) parts of the (Myrtus
communis L.) (root, stem, leaves, fruits) with concentrations (25,50,75,100%)
was carried out against growth of Gr- and Gr+ bacteria which were isolated and
identified
from the pathogenic specimens of The University Children's Hospital
bacterial laboratory in Damascus, using agar well diffusion method on Mueller
– Hinton medium.
Activity of Ziziphora tenuior L. leaves organic extracts against four pathogenic
bacteria, which were isolated from clinical samples and prepared from Al-Assad hospital
laboratory in Lattakia, were tested by disc diffusion method through 2014.
Res
ults showed that Ziziphora tenuior L. leaves extracts have antibacterial activity
against all Gram positive and negative bacteria. Inhibition zones were recorded between
9mm against Pseudomonas aeruginosa and 44mm against Staphylococcus aureus at
concentration of 200mg/ml for the methanolic extract. Whereas the inhibition zones for the
ethanolic extract were recorded between 7mm at concentration of 40mg/ml against
Streptococcus sp. and 34mm at concentration of 200mg/ml against Staphylococcus aureus.
These results represent the importance of Ziziphora tenuior L. as a source of natural
substances counteraction of pathogenic bacteria.
This study was conducted at the laboratory of Food Science Department,
Agriculture College, Damascus University to isolate and characterize of
Lactobacilli bacteria from some Syrian foods and detection the effectiveness of
anti-pathogenic bacteria
l growth. Lactic acid bacteria from different dairy
products, pickles and dried plant samples were isolated and identified
according to common characteristics. Results showed that twenty-three isolates
were rods while only 2 of the them were coccid. These isolates were tested for
their antimicrobial effect against pathogenic bacteria for both gram positive
bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella
pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial
effect against the three tested bacteria, of which CP50 and CP46 isolates
showed the best antimicrobial effect. Results of the standard physiological and
biochemical tests identified fourteen isolates as Lactobacillus plantarum and one
isolate as Lactobacillus fermentum.
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