Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userOptimization of β-galactosidase Production by local sporeforming bacteria isolated from Milk
أمثلة إنتاج أنزيم β-galactosidase من بكتيريا محلية متبوغة معزولة من الحليب
1699
0 20
0
(
0
)
Added by
Aِl-Baath University ورقة بحثية
Publication date
2017
fields
Food Sciences
and research's language is
العربية
Created by
Shamra Editor
Ask ChatGPT about the research
جمعت 40 عينة حليب من محافظة حمص لعزل البكتيريا المتبوغة و دراسة قدرتها على إنتاج أنزيم β-galactosidase و تشخيص العزلة الأكثر كفاءة في إنتاج الأنزيم, أظهرت النتائج وجود 25 عزلة منتجة لأنزيم β-galactosidase حيث قدرت فعالية الβ-galactosidase باستخدام جهاز الطيفي الضوئي عند طول موجة420nm.
40 samples were collected from milk from Homs to isolate the bacteria and study their ability to produce the enzyme β- galactosidase and diagnose isolates the most efficient in the production of the enzyme, the results showed the presence of 25 sporeforming isolates producing the enzyme β-galactosidase , the effectiveness of β-galactosidase was estimated using spectrophotometer at a wavelength of 420 nm.
References used
ACAN, N, 2011- High level production of extracellular β- galactosidase from Bacillus licheniformis ATCC 12759 in submerged fermentation. African Journal of Microbiology Research, 5, 4615-4621
AMMINI, P, KIRAN, K, JIYA, J, NEETHA, J, SANTHA, N, 2009- Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin. Braz. J. Microbiol, India, 40, 269-275
ARTOLOZAGA, M, JONES, R, SCHNRIDER, A, FURLAN, S, CARVALLO,F, 1998- Bioseparation. 7, 137-143
rate research
Read More
Some characteristics of β-galactosidase enzyme that was isolated from a new
born goat brain were studied. This study concluded that the enzyme is glucoenzyme
in which the carbohydrate part constitutes 22.1% in accordance with
phenol –sulfate acid
method.
The optimum pH for the enzyme activity is 5.5. The enzyme lost its activity
completely at pH8.5, and showed great stability at the range of pH 4-6.
The results indicated that the optimum temperature for the enzyme activity
is 55Co at the optimum pH. The stability temperature for the enzyme is
35-60Co.
The analytical results of 5%lactose solution hydrolyzed by the enzyme have
indicated that the hydrolysis rate is between 40% after 60 minutes, to 95%
after 270 minutes.
β-galactosidase enzyme was isolated from the new born goat brain by nine
methods, It was found that the sodium acetate 0.2 Mole/Liter +0.2Mole/Liter
NaCl PH5 method have given the highest specific activity of crude enzyme in
comparison with the ot
her methods. Also, this enzyme was purified by using
four methods, the second one (cold acetone) was the butter. As a result the
purification fold was about 135.46 times and the yield about 77.14% by using
Sephacryl S200 (second step). This enzyme is 187.437 KDa as a molecular
weight.
B-D-Galactosidase (B-D- galactoside galactohydrolase , E. C. 3.2.1.23) , is one of the most important enzymes used in food processing . It a specific example of a glycosidase enzyme. Many organisms naturally synthesize B- galactosidase , including mi
croorganisms , plants and animal cells . Among these possibilities , microbial sources are the best.
45 samples were collected from various sources (soil, degraded wood and
mushroom compost) from three cities (Damascus, Homs and Lattakia). 18
Trichoderma isolates were isolated and identified by microscope. These isolates
were screened using CMC m
edium with Congo red dy to identify their ability
to produce cellulase complex. The amount of enzyme production was
determined depending on the radius of clear zone around the colony. Results
showed that the most productive isolate was Tr with radius 7 ± 0.2 cm followed
by Tg and Ti. Optimization of cellulase production was performed using
response surface methodology (RSM). The optimal parameters were:
temperature 29.5 ˚C, pH = 6، incubation time 4 days, aeration speed 175 rpm
and wheat straw concentration 3%. All studied parameters had significant
effect on cellulase enzyme activity.
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
