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Detection of Lactic Acid Bacteria Isolated from Syrian Dairy Products

الكشف عن بكتريا حمض اللبن المعزولة من بعض منتجات الألبان السورية

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 Publication date 2006
and research's language is العربية
 Created by Shamra Editor




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This research was conducted in the Agriculture Faculty- Food Science Department, Damascus University, for detecting Lactic acid bacteria isolated from Syrian dairy products (white fresh cheese-Leben), Samples were collected from August, 2004 to October, 2005 from different areas in Syria. Analysis was done for 245 bacterial isolates from 40 samples of white fresh cheese and 58 samples of yogurt (curd). Results showed that the genus Enterococcus was dominant with a percentage of 47.52%; Pediococcus had a percentage of 26.3 %; while Lactococcus had a percentage of 15.38% with only 3.2% for Lactobacillus.


Artificial intelligence review:
Research summary
أجرى هذا البحث في كلية الزراعة - قسم علوم الأغذية بجامعة دمشق، بهدف الكشف عن بكتيريا حمض اللاكتيك المعزولة من منتجات الألبان السورية (الجبن الأبيض الطازج واللبن). تم جمع العينات من أغسطس 2004 إلى أكتوبر 2005 من مناطق مختلفة في سوريا. تم تحليل 245 عزلة بكتيرية من 40 عينة من الجبن الأبيض الطازج و58 عينة من اللبن. أظهرت النتائج أن جنس Enterococcus كان الأكثر هيمنة بنسبة 47.52%، بينما كان Pediococcus بنسبة 26.3%، وLactococcus بنسبة 15.38%، وLactobacillus بنسبة 3.2% فقط.
Critical review
دراسة نقدية: البحث يقدم معلومات قيمة حول توزيع بكتيريا حمض اللاكتيك في منتجات الألبان السورية، ولكنه يفتقر إلى تحليل أعمق حول تأثير هذه البكتيريا على جودة وسلامة المنتجات. كما أن الدراسة لم تتناول الجوانب الاقتصادية أو البيئية المرتبطة بإنتاج هذه المنتجات. كان من الأفضل تضمين المزيد من التفاصيل حول الطرق المستخدمة في التحليل وأي تحديات واجهت الباحثين خلال الدراسة.
Questions related to the research
  1. ما هي الفترة الزمنية التي تم فيها جمع العينات؟

    تم جمع العينات من أغسطس 2004 إلى أكتوبر 2005.

  2. ما هو الجنس البكتيري الأكثر هيمنة في العينات؟

    الجنس البكتيري الأكثر هيمنة هو Enterococcus بنسبة 47.52%.

  3. كم عدد العزلات البكتيرية التي تم تحليلها في الدراسة؟

    تم تحليل 245 عزلة بكتيرية.

  4. ما هي النسبة المئوية لجنس Lactobacillus في العينات؟

    النسبة المئوية لجنس Lactobacillus في العينات هي 3.2%.


References used
Beukes, E. M., Bester, B. H. and Mostert, J. F. (2001). «The microbiology of South African traditional fermented milks». International Journal of Food Microbiology. 63, 189–197
Corsetti, A., Gobbetti, M., Smacchi, E., De Angelis, M. and Rossi, J. (1998). «Accelerated ripening of Pecorino Umbro cheese».J. Dairy Res. 65, 631-642
Dicks, L. M. T., DuPlessis, E. M., Dellaglio, F. and Lauer, E. (1996). «Reclassification of Lactobacillus casei subsp. casei ATCC 393 and Lactobacillus rhamnosus ATCC 15820 as Lactobacillus zeae nom. rev., designation of ATCC 334 as the new type of L. casei subsp. casei, and rejection of the name Lactobacillus paracasei» Int. J. Syst. Bacteriol. 46,337-340
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This research was conducted at the Faculty of Agriculture, Department of Food Science laboratories to isolate lactic acid bacteria from some Syrian milk products (milk, yogurt, cheese) and determine some morphological, physiological characteristic and the ability of these isolates to ferment sugars.
Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.
Thirty strains of lactic acid bacteria were isolated from different local fermented foods. Isolates were identified by using morphological, cultural and biochemical tests using the API 50-CHL and API 20 Strep systems. Preliminary characterization tests showed that 33.33% of the isolates were rod shaped and 66.67% belonged to cocci bacteria.
Four strains of lactic acid bacteria (LAB) from different genus، species and sources were tested for their abilities to bind of AFM1 for 14 hours. Lactobacillus rhamnosus LC705 showed higher ability to bind AFM1 (88.35%)، followed by Bifidobacteri um bifidum BG1 (84.21%), Lactobacillus bulgaricus R21 by 80.41% and finally Streptococcus thermophilus by 71.52% after 14 hours of incubation.
Sixty yeasts spp. were isolated from samples of whey which collected from different locations in Syria. These yeasts were classified morphologically according to Barnett’s and physiologically by API technique to identify the species of Yeasts, whe re had found 10 classified yeasts as follows: Candida krusei is the most of yeasts which founded in whey with 21.66 %, and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came (Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with 11.66%, after that the Candida magnolia is ranked in the fourth rank by distributing with 8.33%. as soon as the Candida famata came in the fifth rank with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%, finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank with 3.33%, so we noticed the variety of the yeasts in the studied locations in Syria.
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