جمعت 45 عينة تتضمنت عينات تربة و خشباً متحللاً و كمبوست فطر زراعـي مـستنزفاً مـن ثـلاث
محافظات (دمشق، حمص، اللاذقية). شخصت منها مجهرياً 18 عزلة من جنس الفطـر Trichoderma
و غُربلتْ العزلات من حيث قدرتها على إنتاج إنزيمات السلولاز من خلال تنميتها على أطباق بتري حاويـة
على آغار كربوكسي ميتيل السلولوز كمصدرللكربون مدة 5 أيام، ثم حدد مقدار إنتاج الإنـزيم بالاعتمـاد
على الهالة الشفافة المتشكلة حول المستعمرات بعد إضافة صبغة أحمر الكونغـو. أوضـحت الدراسـة أن
العزلات Ti ،Tg ،Tr كانت الفُضلى في إنتاج السلولاز مقارنة بباقي العزلات، و تميزت العزلة Tr بـأعلى
كفاءة إنزيمية إذْ وصل متوسط قطر الهالة حول المستعمرة إلى 7 ± 2.0 سم، ثم جـرت أمثلـة ظـروف
الإنتاج في الوسط السائل باستخدام التـصميم الإحـصائي (RSM (methology surface response،
فكانت درجة الحرارة المثلى 50.29˚م و درجة الحموضة المثلى pH = 6 و أفضل مدة تحـضين 4 أيـام،
و أفضل سرعة دوران 175 دورة في الدقيقة و نسبة الركيزة (مسحوق تبن القمح) المثلـى 3 % و بينـت
النتائج تأثيراً معنوياً للقيم المدروسة لكل من درجة الحرارة، و درجة الحموضة، و مدة التحضين، و سرعة
التهوية، و تركيز الركيزة في فعالية الإنزيم.
45 samples were collected from various sources (soil, degraded wood and
mushroom compost) from three cities (Damascus, Homs and Lattakia). 18
Trichoderma isolates were isolated and identified by microscope. These isolates
were screened using CMC medium with Congo red dy to identify their ability
to produce cellulase complex. The amount of enzyme production was
determined depending on the radius of clear zone around the colony. Results
showed that the most productive isolate was Tr with radius 7 ± 0.2 cm followed
by Tg and Ti. Optimization of cellulase production was performed using
response surface methodology (RSM). The optimal parameters were:
temperature 29.5 ˚C, pH = 6، incubation time 4 days, aeration speed 175 rpm
and wheat straw concentration 3%. All studied parameters had significant
effect on cellulase enzyme activity.
References used
Adney, B. and J. Baker. 2008. Measurement of cellulase activities, Department of Energy Office of Energy Efficiency and Renewable Energy, National Renewable Energy Laboratory (NREL), Colorado, 1-8
Adsul, M. G., K. B. Bastawde, A.J.Varma and D. V. Gokhale. 2007. Strain improvement of Penicillium janthainellum NCIM 1171 for increased cellulase production. Bioresource Technology, 98, (7): 1467-1473
Aehle, W. 2007. Enzymes in Industry, Production and Applications. The Netherlands
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
Forty Isolates of Streptomyces bacteria were obtained from soil samples
from different regions of Syria (Damascus, Sweida, Daraa, Homs, Latakia,
Damascus, Hasakeh, Deir-ezzor) during 2012. The isolates were identified using
ISP (International Stre
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
Producing of biofuel from crops wastes is considered one of the
advanced eco- friendly technologies. This research was conducted
in the General Commission For Biotechnology in order to study the
possibility to use the juice of sugar sorghum plant
B-D-Galactosidase (B-D- galactoside galactohydrolase , E. C. 3.2.1.23) , is one of the most important enzymes used in food processing . It a specific example of a glycosidase enzyme. Many organisms naturally synthesize B- galactosidase , including mi