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Register a new userاستحداث الكالس وإعادة تشكيل النبات الكامل من خلال زراعة المآبر و الأبواغ المعزولة لبعض أصناف الحمص (Cicer arietinum L )
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Tishreen University رسالة ماجستير
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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Shamra Editor
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استحداث الكالس وإعادة تشكيل النبات الكامل من خلال زراعة المآبر و الأبواغ المعزولة لبعض أصناف الحمص (Cicer arietinum L )
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رقيه نزيه سعد فؤاد محاصيل الحبوب والبقول منشورات جامعة تشرين
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This research was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture, Tishreen University. Because of the importance of the food and economic of chickpea, two Winter chickpea cultivars (Ghab 4, Ghab 5) and two Vernal chickpea culti
vars were used (ILC263, ILC1929) and planted in pots until flowering, so the floral buds were picked before opened and washed with distilled water and alcohol three times. Anthers were separated and treated at preliminary temperatures (4 Cْ for 48 hours, and 35 Cْ for 12 hours), then it washed with a solution of hypochlorite of sodium( Naocl 2%) for a period of 15 minute, and washed with distilled sterilized water ,then where planted on the environment Murashige and Skoog ( MS) equipped with 1, 3 and 5 mg/L of auxin 2,4, D, as well as 0.1, 0.2 and 0.3 mg /L of Cytokinin Benzyl Amino Purine( BAP) individually and with interaction between them and incubated under conditions of 27 Cْ and 75% of the humidity and the intensity of 1500 Lux of light for 16 hours. The main objective of research was to study the effect of both preliminary heating treatment , quality and concentration of used hormone on producing callus from used chickpea cultivars anthers. Results showed difference in cultivars response in the treatment of both temperature and single hormone, so Ghab 5 cultivar was the most responsive to the formation of callus, while the results indicated that the highest percentage of the formation of callus was 40% when treated at 4 Cْ of Ghab 4 and Ghab 5 cultivars, while ILC263 cultivar showed less response in all the individual treatments for growth regulators.
As well as results showed that the significant and catalyst role for using of hormones together with heat treatment, where each of Ghab 5, Ghab 4 and ILC1929 cultivars were the superiority, by the arrival of the percentage of 80% and 60%, respectively.
A half diallel making design was used to determine combining ability and heterosis of six
chickpea lines and their hybrid combinations with R.C.B.D. in three replication for seed
yield per plant, number of pods per branch, pods number per plant, fi
rst pod height and
100- seed weight, during 2015 and 2016 seasons, at the research Center of Al-Gab
(G.C.S.A.R). Genotypes, general (GCA) and specific (SCA) combining ability mean
squares were significant for all studied traits. The ratio σ2
GCA / σ2
SCA were detected for all
traits and showed that non- additive gene action was more important than additive gene
action in controlling all studied traits. GCA effects showed that the lines P2(IL.10158) was
good general combiner for number of pods per branch and plant, also, 100- seed weight
where, P3 (IL.5883) and P4(IL.4) were good general combiner for first pod height and seed
yield per plant, respectively. SCA effects showed that(P1×P2) hybrid was the best F1
crosses combination for seed yield per plant. On the other side (P1×P2), (P1×P4) and
(P2×P4) were showed positive and significant heterosis values for seed yield per plant and
100- seed weight also first pod height and number of pod per branch and plant
respectively.
This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorgh
um, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
A study was conducted to evaluate the diversity among chickpeas accessions
collected from Jordan for some morpho-agronomic traits, specially yield and its
components. The evaluated material consisted of 137 accessions and three
improved cultivars
released in Jordan. The study was conducted at the Jordan
University of Science and Technology Research Station using the augmented
design. Some of the parameters used in the evaluation included the mean,
standard deviation, range, coefficient of variation, diversity index. Also, a
dendrogram to establish the relationship among locations where landraces
were originally developed.
Results indicated the presence of significant variability for all traits under
investigation. Diversity index values (H′) exceeded 0.50 for all characters with
the highest value for biological yield (0.84), followed by lowest pod height
(0.82), grain yield (0.78), and straw yield (0.78).
The following accessions were found to be superior over the improved
cultivars in one or more of the studied characters: ILC 6941, ILC 6938, ILC
4411, and ILC 4096. These are important genetic resources for breeders to
improve the chickpea crop.
The results indicated the importance of the local landraces as a source of
genetic variability that should be conserved, evaluated and utilized by breeders
to improve the crop productivity and quality.
Chickpea (Cicer arietinum L.) in the Mediterranean climate can experience
two types of drought: intermittent drought stress and terminal drought stress.
A study was conducted during early August 2002 to early April 2003 under
glasshouse conditions
, to study the response of six chickpea genotypes, four
Kabuli and two Desi, to soil moisture levels.
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