This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorgh
um, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
Ssilverleaf nightshade (Solanum elaeagnifolium Cav.) is a serious weed to
cotton and other field summer crops due to the alkaloids present in the plant
and have allelopathic potential on the germination of other plants. Sorghum
(Sorghum bicolor) h
ave been investigated as a allelopathic crop having strong
competition potential in the field containing many numerous water solution
allelochemicals and can be used to control weeds in field crops. A field
competition study between sorghum and silverleaf nightshade plants was
conducted to determine how long every plant can resist against the other.
This study was conducted at the General Commission Of Scientific
Agricultural Research (GCSAR) for tow consecutive years, 2000-2001, to
estimate the heterosis of some sorghum hybrids utilizing cytoplasmic male
sterility technique, and to determine
the behavior of the parents used in
hybridization concerning their relation to the cytoplasmic male sterility.
Hybrids were planted in a randomized complete block design, including 43
hybrids in tow replications.
The results showed variation among parents characters, because they were
selected from different environments and also differ in genetic components.
positive heterosis was recorded form crosses involved foreign parents with
local ones, or foreign hybridized with foreign. positive heterotic values for
earliness and productivity were obtained .
The genetic constitution of the male parent was assessed as R-line (restoring
of the A-line), and B-line (maintainer of the A-line).
