Nineteen locally selected potato lines were screened for water stress
tolerance by measuring the growth parameters in vitro. The stress was achieved
by adding 4% of sorbitol to the growth medium. Plant height and diameter,
leaf area, number, lengt
h and diameter of roots and plant fresh and dry weight
were measured. The results showed a difference in the response of the studied
lines to water stress, cluster analysis, based on the sum of all growth
parameters relative values of the lines showed that the lines were divided into
three groups according to water stress tolerance: The tolerant group, the
moderate tolerant group and the sensitive group. The results indicated the
possibility to use the in vitro method to screen the potato lines for water stress
tolerance.
This research was conducted at the Agriculture Faculty, Damascus
university to determine some factors affecting rooting of some grape rootstocks
(B41, Ru140, SO4) by hardwood cutting during two subsequent seasons. The
results showed that Rooting p
ercentage ranged between 50 to 65%. with
significant differences between tested rootstocks. The highest rooting
percentage was obtained on B41 rootstock reached 65.47% followed by SO4
and then by Ru140 which gave the lowest percentage 50.44%. The treatment
with hormone IBA increased rooting proportion and all the parameters
(number and length of root, number of leaves and shoot length) were
significantly higher than parallel parameters of the control. The storage of
cuttings at 4°C for 50 days increased significantly proportion of rooting and
number of roots compared with the storage under field condition. There were
significant differences among dates of planting where the third date (mid
February) showed superiority in all parameters studied over other dates. The
cuttings from basal and middle of annual growth gave a significant superiority
in the average root length (5.26–4.32 cm, successively) compared to the
terminal cuttings while there was no significant effect to the cuttings position on
rooting proportion.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
In this study, the effect of different concentrations of sodium chloride
(NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some
growth indicators of SO4 grape rootstock propagated in vitro was studied at the
laboratories of Gen
eral Commission for Scientific Agricultural Research
(GCSAR), Damascus/Syria. Results indicated that the increase of NaCl
concentration up to 100 mM in culture medium led to reduce the growth
indicators (survival plants, average of the plant length and average number of
buds) with significant differences when compared with the control plants after
4 weeks from culturing on multiplication media, while treatment with 150 mM
of sodium chloride led to death of all plants. Using the concentrations 50 and
100 mM of NaCl also caused decreasing the total chlorophyll content in the
leaves.
The research was conducted on some olive
varieties known as Alsourani- Aldan- Mahazm Abu Satel- Alglet-
Alnepali, in the aim of comparing their tolerance against the stress
of salinity (sodium chloride) through some morphological and
physiological traits. Different concentrations of salt as well as to
the control (fresh water) were used in this experiment.