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Register a new userاستخدام الأحماض الدهنية في تصنيف بعض أنواع البكتريا والخمائر المعزولة محلياً من الأغذية
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Damascus University أطروحة دكتوراه
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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صنفت 1165 عزلة من البكتيريا والخمائر من عينات غذائية مختلفة باستخدام تقانة API ,شملت 342 عزلة من بكتيريا سالبة غرام
No English abstract
References used
Werkman, C.H.: Wilson,P. W. (2013) : Bacterial Physiology. P. 9-12
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تعد البكتريا الممرضة المنقولة عن طريق الغذاء سبباً للامراض والوفيات وقد قاومت العديد من الجهود المبذولة للكشف عنها وتشخيصها .
Sixty yeasts spp. were isolated from samples of whey which collected from
different locations in Syria. These yeasts were classified morphologically
according to Barnett’s and physiologically by API technique to identify the
species of Yeasts, whe
re had found 10 classified yeasts as follows:
Candida krusei is the most of yeasts which founded in whey with 21.66 %,
and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came
(Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with
11.66%, after that the Candida magnolia is ranked in the fourth rank by
distributing with 8.33%. as soon as the Candida famata came in the fifth rank
with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%,
finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank
with 3.33%, so we noticed the variety of the yeasts in the studied locations in
Syria.
This research aimed to study the effect of various growth conditions
(temperature, culture age and culture medium) on the fatty acids composition
of cytoplasm membrane of 37 isolates of Staphylococcus aureus using Gas
Chromatography-Mass Spectrome
try (GC-MS). Results revealed that the total
number of fatty acids of Staph. aureus was 33. Eight of them were principle and
included: anti-isotetradecanoic acid (aC15:0), octadecanoic acid (C18:0),
eicosanoic acid (C20:0), isotetradecanoic acid (Ci14:0), isotridecanoic acid
(Ci15:0), hexadecanoic acid (C16:0), antisohexa- decanoic acid (Ca17:0) and
octadecenoic acid (C18:1) with a percentage of 33.96, 18.37, 14.94, 7.32, 6.23,
4.14, 3.03 and 2.89%, respectively. The percentage of each of the other fatty
acids was less than 1%. Increasing the incubation temperature was associated
with reducing the percentages of Ca15:0, Ci15:0, C16:0 and C18:1; and
increasing Ca17:0, Ci14:0, C18:0 and C20:0. The increase in incubation period
caused a decrease in C16:0, C18:0, C18:1 and C20:0; and an increase in Ca15:0
and Ca17:0. Moreover, the variation in culture media caused differences in the
percent of Ca15:0, C18:0 and C20:0; while the other fatty acids showed no
changes.
The experiment was conducted on 24 brown laying hens at 30
weeks old and were randomly assigned to four groups, each
consisting of six laying hens and put in individual cages stacked in
accordance with the battery system in the unit of poultry fee
d at the
Faculty of Veterinary Medicine, University of Hama, in order to
study the effect of different types of oil and fat on performance,
apparent and true metabolizable energy, and apparent and true fats
digestibility.
The research aims to Study the fatty acids composition of each of (Rapeseed-
Borage-Soya bean) Oil Syrian Gas Chromatography-invasive manner, and compare the
results with global studies of the same studied oils, and shed light on both the Syrian
o
ils(Rapeseed- Borage) to be used as substitutes or mixtures of health benefit or as a drug.
the study showed that the importance of the oil lies in the amount of the percentages of
unsaturated fatty acids UFA and Polyunsaturated fatty acids PUFA and Monounsaturated
fatty acids MUFA, with the highest values in the UFA installation Syrian oils to Rapeseed
oil < Borage oil < Soya bean oil, search results compared Rapeseed oil and improved
processor and the highest percentage of genetically MUFA also showed the back of Syrian
oil Borage percentage of MUFA less, but The best percentage of PUFA clear appeared in
the oil Borage.
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