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Register a new userThe effect of growth conditions on fatty acids composition of cytoplasm membrane of Staphylococcus aureus
تأثير شروط النمو في تركيب الأحماض الدهنية لبكتريا Staphylococcus aureus
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هدف البحث إلى دراسة تأثير شروط النمو المختلفة (درجة الحرارة، و عمـر المـستعمرة و وسـط الزرع) في تركيب الأحماض الدهنية للغشاء الـستوبلازمي لــ 37 عزلـة لبكتريـا aureus. Staph باستخدام جهاز الاستشراب الغازي المزود بوحدة مطياف الكتلة (MS-GC) . أظهرت النتائج بـأن عـدد الأحماض الدهنية لبكتريا aureus. Staph قد بلغ 33 حمضاً ، و كانت الأحماض الدهنية الرئيسة الثمانية هــي انتـي ايـزو تيتراديكانويـك (0:Ca15) و اوكتاديكانويــك (0:C18) و ايكوس ـانويك (0:C20) و إيزوتيتراديكانويك (0:Ci15) و ايزو تريديكانويك (0:Ci14) و هكساديكانويك (0:C16) و انتـي ايـزو 7.32 و 14.94 و 18.37 و 33.96 بلغـت و بنسب (C18:1) و اوكتاديسينويك (Ca17:0) هكساديكانويك و 2.6 و 14.4 و 03.3 و 9.2 % على التوالي، في حين كانت نسبة الأحماض الدهنية الأخرى أقل من 1% لكل منها. أدى ارتفاع درجة حرارة التحـضين إلـى تنـاقص كـل مـن 0:Ca15 و 0:Ci15 و 0:C16 و 1:C18 و زيادة كل من 0:Ca17 و 0:Ci14 و 0:C18 و 0:C20 ، و أدت زيادة عمر المستعمرة تناقص الزرع وسط اختلاف أن عن فضلاً، Ca17:0 و Ca15:0 و زيادة C20:0 و C18:1 و C18:0 و C16:0 أدى إلى تباين في نسبة الأحماض الدهنية لكل من 0:Ca15 و 0:C18 و 0:C20 ، و لم تلاحـظ تغيـرات واضحة في نسب بقية الأحماض الدهنية مع تغير شروط النمو.
This research aimed to study the effect of various growth conditions (temperature, culture age and culture medium) on the fatty acids composition of cytoplasm membrane of 37 isolates of Staphylococcus aureus using Gas Chromatography-Mass Spectrometry (GC-MS). Results revealed that the total number of fatty acids of Staph. aureus was 33. Eight of them were principle and included: anti-isotetradecanoic acid (aC15:0), octadecanoic acid (C18:0), eicosanoic acid (C20:0), isotetradecanoic acid (Ci14:0), isotridecanoic acid (Ci15:0), hexadecanoic acid (C16:0), antisohexa- decanoic acid (Ca17:0) and octadecenoic acid (C18:1) with a percentage of 33.96, 18.37, 14.94, 7.32, 6.23, 4.14, 3.03 and 2.89%, respectively. The percentage of each of the other fatty acids was less than 1%. Increasing the incubation temperature was associated with reducing the percentages of Ca15:0, Ci15:0, C16:0 and C18:1; and increasing Ca17:0, Ci14:0, C18:0 and C20:0. The increase in incubation period caused a decrease in C16:0, C18:0, C18:1 and C20:0; and an increase in Ca15:0 and Ca17:0. Moreover, the variation in culture media caused differences in the percent of Ca15:0, C18:0 and C20:0; while the other fatty acids showed no changes.
References used
Annous, B.A.,M. F. Kozempeland M. J. Kurantz. 1999. Changes in membrane fatty acid composition of Pediococcus sp. Strain NRRL B-2354 in response to growth conditions and its effect on thermal resistance. Appl. and environ. Microbio., 65(7):2857–2862
Behme, R. J., R. Shuttleworth, A. Mcnabb and W. D. Colby. 1996. Identification of Staphylococci with a self-educating system using fatty acid analysis and biochemical tests. J. clin. Microbio.., Pp: 3075–3084
Bezbaruah, R. L., B. K. G. Pillai and J. Baruah. 1988. Effect of growth temperature and media composition on the fatty acid composition of Bacillus stearothermophilus AN 002. Antonie van Leeuwenhoek 54:37-45
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– Damascus University in the Department of Food Sciences. The aim of this
research use to study the inhibition effect of the growth and activity of Lactic
acid bacteria
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strains of Staph. aureus and Lactic acid bacteria was used the effect of Lactic
acid bacteria (Streptococcus thermophilus and Lactobacillus bulgaricus) on the
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Staph. aureus was fixed then was decreased gradually according to time. And
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bulgaricus were used as a mixture starter, then when was used as a single
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results with global studies of the same studied oils, and shed light on both the Syrian
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ils(Rapeseed- Borage) to be used as substitutes or mixtures of health benefit or as a drug.
the study showed that the importance of the oil lies in the amount of the percentages of
unsaturated fatty acids UFA and Polyunsaturated fatty acids PUFA and Monounsaturated
fatty acids MUFA, with the highest values in the UFA installation Syrian oils to Rapeseed
oil < Borage oil < Soya bean oil, search results compared Rapeseed oil and improved
processor and the highest percentage of genetically MUFA also showed the back of Syrian
oil Borage percentage of MUFA less, but The best percentage of PUFA clear appeared in
the oil Borage.
This study was conducted in the years 2008-2009-2010, it has analyzed for
Staph. aureus and isolated from 100 samples of traditional ice cream sold to
customers it has had collected from different regions parts of Damascus and his
countryside. The
study has been found that there was no violation of the Syrian
standard for Staph. aureus. And the results were also diagnosed using the
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The broodstock of Blue tilapia (Oreochromis aureus) were reared in glass aquaria in the laboratory of the High Institute of Marine Research to study the effect of different water salinities on growth rates of those broodstocks and comparing the resul
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and glutamine decreased in semimembranous muscle compared with dorsal rectangular
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