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The effect of growth conditions on fatty acids composition of cytoplasm membrane of Staphylococcus aureus

تأثير شروط النمو في تركيب الأحماض الدهنية لبكتريا Staphylococcus aureus

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 Publication date 2012
and research's language is العربية
 Created by Shamra Editor




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This research aimed to study the effect of various growth conditions (temperature, culture age and culture medium) on the fatty acids composition of cytoplasm membrane of 37 isolates of Staphylococcus aureus using Gas Chromatography-Mass Spectrometry (GC-MS). Results revealed that the total number of fatty acids of Staph. aureus was 33. Eight of them were principle and included: anti-isotetradecanoic acid (aC15:0), octadecanoic acid (C18:0), eicosanoic acid (C20:0), isotetradecanoic acid (Ci14:0), isotridecanoic acid (Ci15:0), hexadecanoic acid (C16:0), antisohexa- decanoic acid (Ca17:0) and octadecenoic acid (C18:1) with a percentage of 33.96, 18.37, 14.94, 7.32, 6.23, 4.14, 3.03 and 2.89%, respectively. The percentage of each of the other fatty acids was less than 1%. Increasing the incubation temperature was associated with reducing the percentages of Ca15:0, Ci15:0, C16:0 and C18:1; and increasing Ca17:0, Ci14:0, C18:0 and C20:0. The increase in incubation period caused a decrease in C16:0, C18:0, C18:1 and C20:0; and an increase in Ca15:0 and Ca17:0. Moreover, the variation in culture media caused differences in the percent of Ca15:0, C18:0 and C20:0; while the other fatty acids showed no changes.


Artificial intelligence review:
Research summary
تهدف هذه الدراسة إلى تحليل تأثير شروط النمو المختلفة (درجة الحرارة، وعمر المستعمرة، ووسط الزرع) على تركيب الأحماض الدهنية في غشاء السيتوبلازم لبكتيريا Staphylococcus aureus باستخدام جهاز الاستشراب الغازي المزود بوحدة مطياف الكتلة (GC-MS). أظهرت النتائج أن عدد الأحماض الدهنية في بكتيريا Staph. aureus بلغ 33 حمضًا، وكانت الأحماض الدهنية الرئيسية هي: انتي ايزو تيتراديكانويك (Ca15:0)، اوكتاديكانويك (C18:0)، ايكوسانوك (C20:0)، ايزو تيتراديكانويك (Ci14:0)، ايزو تريديكانويك (Ci15:0)، هكساديكانويك (C16:0)، انتي ايزو هكساديكانويك (Ca17:0) واوكتاديسينويك (C18:1). أظهرت الدراسة أن زيادة درجة حرارة التحضين تؤدي إلى انخفاض نسب بعض الأحماض الدهنية وزيادة نسب أخرى، كما أن زيادة عمر المستعمرة تؤدي إلى تغييرات في نسب الأحماض الدهنية. بالإضافة إلى ذلك، أظهر اختلاف وسط الزرع تأثيرًا على نسب بعض الأحماض الدهنية بينما لم تتغير نسب الأحماض الأخرى بشكل كبير.
Critical review
دراسة نقدية: الدراسة تقدم معلومات قيمة حول تأثير شروط النمو المختلفة على تركيب الأحماض الدهنية في بكتيريا Staphylococcus aureus، ولكن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن توسيع نطاق العينات لتشمل أنواع أخرى من البكتيريا لمقارنة النتائج. ثانيًا، الدراسة لم تتناول بشكل كافٍ تأثير العوامل البيئية الأخرى مثل درجة الحموضة والملوحة، والتي قد تكون لها تأثيرات مهمة على تركيب الأحماض الدهنية. أخيرًا، كان من الممكن تقديم تحليل أعمق للنتائج وربطها بالتطبيقات العملية في مجالات مثل الطب والصناعة الغذائية.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي هو دراسة تأثير شروط النمو المختلفة (درجة الحرارة، وعمر المستعمرة، ووسط الزرع) على تركيب الأحماض الدهنية في غشاء السيتوبلازم لبكتيريا Staphylococcus aureus.

  2. ما هي الأحماض الدهنية الرئيسية التي تم تحديدها في بكتيريا Staphylococcus aureus؟

    الأحماض الدهنية الرئيسية هي: انتي ايزو تيتراديكانويك (Ca15:0)، اوكتاديكانويك (C18:0)، ايكوسانوك (C20:0)، ايزو تيتراديكانويك (Ci14:0)، ايزو تريديكانويك (Ci15:0)، هكساديكانويك (C16:0)، انتي ايزو هكساديكانويك (Ca17:0) واوكتاديسينويك (C18:1).

  3. كيف تؤثر درجة حرارة التحضين على نسب الأحماض الدهنية في بكتيريا Staphylococcus aureus؟

    زيادة درجة حرارة التحضين تؤدي إلى انخفاض نسب بعض الأحماض الدهنية مثل Ca15:0 وCi15:0 وC16:0 وC18:1، وزيادة نسب أخرى مثل Ca17:0 وCi14:0 وC18:0 وC20:0.

  4. ما هو تأثير عمر المستعمرة على تركيب الأحماض الدهنية في بكتيريا Staphylococcus aureus؟

    زيادة عمر المستعمرة تؤدي إلى انخفاض نسب الأحماض الدهنية C16:0 وC18:0 وC18:1 وC20:0، وزيادة نسب الأحماض الدهنية Ca15:0 وCa17:0.


References used
Annous, B.A.,M. F. Kozempeland M. J. Kurantz. 1999. Changes in membrane fatty acid composition of Pediococcus sp. Strain NRRL B-2354 in response to growth conditions and its effect on thermal resistance. Appl. and environ. Microbio., 65(7):2857–2862
Behme, R. J., R. Shuttleworth, A. Mcnabb and W. D. Colby. 1996. Identification of Staphylococci with a self-educating system using fatty acid analysis and biochemical tests. J. clin. Microbio.., Pp: 3075–3084
Bezbaruah, R. L., B. K. G. Pillai and J. Baruah. 1988. Effect of growth temperature and media composition on the fatty acid composition of Bacillus stearothermophilus AN 002. Antonie van Leeuwenhoek 54:37-45
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