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Register a new userاستخدام الطرائق السريعة في تحديد بعض أنواع البكتيريا الممرضة المعزولة من الاغذية
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Damascus University أطروحة دكتوراه
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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تعد البكتريا الممرضة المنقولة عن طريق الغذاء سبباً للامراض والوفيات وقد قاومت العديد من الجهود المبذولة للكشف عنها وتشخيصها .
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References used
المواصفات القياسية السورية رقم 3455. 2009. طريقة التحري عن جراثيم العطيفة ( الكمبيلوبكتر) الهيئة العامة للمواصفات والمقاييس العربية السورية
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صنفت 1165 عزلة من البكتيريا والخمائر من عينات غذائية مختلفة باستخدام تقانة API ,شملت 342 عزلة من بكتيريا سالبة غرام
The aim of this research consists of the study the possibility of using the
geophysical methods to estimate the shallow geological structures and to show
the detailed lithological changes.
The geoelectric and georadar methods have been implemented
in an area at
Torgau city in Germany.
These methods have been applied on polluted aquiferes. Also geotechnical
study has been implemented in this area to compare the geophysical results.
The results show high resolution in both methods to estimate the
underground geological structures. By the integrated both methods, the
horizontal and vertical changes of these structures have been estimated with
high accuracy.
This study was conducted at the laboratory of Food Science Department,
Agriculture College, Damascus University to isolate and characterize of
Lactobacilli bacteria from some Syrian foods and detection the effectiveness of
anti-pathogenic bacteria
l growth. Lactic acid bacteria from different dairy
products, pickles and dried plant samples were isolated and identified
according to common characteristics. Results showed that twenty-three isolates
were rods while only 2 of the them were coccid. These isolates were tested for
their antimicrobial effect against pathogenic bacteria for both gram positive
bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella
pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial
effect against the three tested bacteria, of which CP50 and CP46 isolates
showed the best antimicrobial effect. Results of the standard physiological and
biochemical tests identified fourteen isolates as Lactobacillus plantarum and one
isolate as Lactobacillus fermentum.
This research was conducted at the Department of Food Science,
Faculty of Agriculture and National commission for posterity
energy. Twenty kg of apple juice concentrate (70%) were tooked
from company of Natural Aljabal Juice from AL-suidaa
Govern
orate and prarerd two concentarate (15% and 35%) by using
distilled –sterlization water according to pirson .
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
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