The purpose of this article is how to use HPLC technique for
quantitive analysis of cholesterol in some foods. The best conditions
are studied for this analysis including composition of mobile phase,
temperature, flow rate, the wavelength of detec
tor UV-VIS, and
acidity of mobile phase. The column used for cholesterol
determination by this method was: C18(5μm,250x4.6mm) with
injection volume of 20μL. Methanol 100% was used as mobile
phase.
The experiment was conducted on 24 brown laying hens at 30
weeks old and were randomly assigned to four groups, each
consisting of six laying hens and put in individual cages stacked in
accordance with the battery system in the unit of poultry fee
d at the
Faculty of Veterinary Medicine, University of Hama, in order to
study the effect of different types of oil and fat on performance,
apparent and true metabolizable energy, and apparent and true fats
digestibility.
The increase in sugar, cholesterol and triglyceride among the citizens of
my country is not insignificant, so I went to study the causes of this
phenomenon. Among these reasons is the genetic disorder that has been
caused by a disorder in the lipid proteins. I studied at least fifty cases of
familial genetic disorder, Cholesterol, and triglyceride levels.
