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Register a new userDetection of Genetic Variation in Malus communis Mother Fields and Within Their Seedlings Using SSR Technique
كشف الخلط الوراثي في حقل أمهات Malus communis و النباتات الناتجة عنه المعدّة لإنتاج غراس التفاح باستخدام تقنية SSR
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المجلة السورية للبحوث الزراعية ورقة بحثية
Publication date
2017
fields
Groves
and research's language is
العربية
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Shamra Editor
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نُفّذ البحث في الهيئة العامة للبحوث العلمية الزراعية في قسم بحوث التفاحيات و الكرمة التابع للهيئة العامة للبحوث العلمية الزراعية، و في مشتل نبع عرى الزراعي، و حقول أمّهات مفعّلة في محافظة السويداء خلال العامين 2011 و2012 و ذلك للكشف عن الخلط الوراثي بين خمسة طرز بذرية في حقول الأمهاتMalus communis و النباتات الناتجة عنها، و المعدّة لإنتاج غراس التفاح، باستخدام عشرة أزواج من بادئات SSR.
This investigation was conducted at Pome and grapevine Division in Sweida, General Commission for Scientific Agriculture Research (GCSAR), and at Ira Agricultural Nursery, during 2011 and 2012 seasons, to detect the genetic variation among five apple seedling genotypes in Mafaala Malus communis fields, and within the seedlings produced from them, using 10 SSR primer pairs.
References used
Al Halabi, O.; and B. Muzher (2015). Assessment of genetic integrity of apple seedlings rootstocks derived from the local apple cultivar (Sukari 2) in Syria using SSR markers. 7th International Scientific Symposium. Agrosym 2015. Book of proceeding. Pp.721-726
Al Hajjar, N.M.; B.M. Muzher; and F. Hamed (2016). Assessing genetic relationships between Pistacia Vera L. Hybrids and their parents (P. Vera X Monocious genotypes of P. Atlantica) using SSR markers. Jordan Journal of Agricultural Sciences. 12(1): 148- 159
Amirbakhtiar, N.; B. Shiran; H. Moradi; and B.E. Sayed-Tabatabaei (2006). Molecular characterization of almond cultivars using microsatellite markers. Acta Horticulture. 726: 51- 56
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The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
This research was carried out in the laboratories of the Atomic
Energy Commission Molecular Biology and Biotechnology
Department in Damascus to study the molecular characterization of
five of local and introduced grapes varieties which collected f
rom
the Pome and Grapevine Research Center in Sweida using 20
primer pairs of SSR.
The wide geographical spread and the exponential growth of the numbers of goats
around the world clearly demonstrate the ability of these ruminants to adapt to
harsh climates and grazing land. The aim of this study was to assess the genetic
divers
ity of 42 samples of Syrian goats from many domestication stations including
Jabali, Shami and hybrid (hybridization between both Shami and Jabali). The study
was done by DNA extraction of these samples, application of SSR technology using
7 microstellite markers. The alleles number of markers were 29 alleles, at a rate of
4.1 allele for each genetic locus. The number of alleles of each locus ranged from 3
alleles in genetically markers (BMS1714, INRAD07, SRCRSP09) to 8 alleles at the
genetic marker SRCRSP01. The rate of allele-frequency across all studied genetic
sites ranged from 0.071429 with the molecular size of 210 bp for the McM527
genetic site to 0.97619 with a molecular size of 55 bp for the SRCRSP09 genetic site.
Morphological Characterization Has Been Achieved For (10) Phenotype Wild
Apple(Malus trilobata (Lab) ) In JABLEH mountains During The Period (2013-2014) In
Five Area (Pichraghe, Helbako, Almonaizlah, Raas al chaara, Aldalia),In Height Between
(780
-1250) m On See Standard, Morphological Characterization Included The
Characteristics Of The Tree, Stem, Shoots, Leaves, Flowers, Fruit And Seed. The Cluster
Analysis For (12) Characteristics Were Divided Into Two Groups Variation Ratio Even (57
%) , The First Group Included Four Types(Two Types Of Rass al chaara(R1, R2), One
Type Of Pichraghe(P1) And One Type Of Almonaizlah(M2) ), Variation Ratio Was The
Least(29.4%) Between (P1) And (R1), The Second Group Included Six Types (Tow
Types Of Pichraghe (P2, P3), Two Types Of Helbako, One Type Of Almonaizlah(M1)
And One Type Of Aldalia (D1).The Second Group Included Tow Sup Group Variation
Ratio Even (52%),The First(P2, P3), And The Second (H1, H2, M1, D1). Variation Ratio
Was The Least(23%) Between (H1) And (H2).
The research was executed in Biotechnology Lab (Faculty of
Agriculture – Damascus University), during the season 2016.
Sixteen genotypes of Sunflower Oil were planted to study the
genetic diversity among these genotypes and to determine the
degree of genetic Agreement using the technique SSR (Simple
Sequence Repeats).
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