أجريت هذه الدراسة في مشفى الأسد الجامعي في اللاذقية، حيث تم جمع 236 عينة اشرشيا قولونية متنوعة مأخوذة من 6 مواضع (بولية، دموية، تناسلية، جراحية، قثطرة، مفجر) وتم اجراء اختبارات التحري عن وجود انزيمات البيتا لاكتاماز واسع الطيف وفقاً لآخر التوصيات الحديثة، حيث اتبعت طرق مرجعية دقيقة تقارب حساسيتها ونوعيتها 99%.
بلغ عدد العينات المقاومة 75 عينة أي 32% من العدد الكلي وبعد اجراء اختبار التحري عن ESBL الذي كان ايجابياً على 62 عينة 26% من العينات الكلية، وأبدت 13عينة فقط مقاومة جرثومية غير ESBL.
Two hundred and thirty-six E. coli isolates n=236 were collected. Antibiotic susceptibility and ESBL production were studied by disc diffusion and DDST (double disk synergy tests) according to Clinical Laboratory Standards Institute guidelines (CLSI) and EUCAST for ESBL.
75 (32%)of total isolates demonstrated MDR (multi drug resistance),62 of resistant bacteria (26% of total) were determined as ESBL producers.
In conclusion, we recommend adding this standard, cheap ESBL detection method to the routine microbial susceptibility tests run in Syrian hospitals in order to determine and isolate the sources of MDR infections and do some effort to contain and eliminate those strains.
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and
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