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Register a new userDetection of Escherichia coli across food samples in Homs
الكشف عن الإشريكية القولونية في عينات غذائية من محافظة حمص
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جمعت عشرون عينة غذائية من منشأ نباتي و حيواني مختلفة المصدر من المحال التجارية، و وضعت ضمن أكياس نايلون عقمة تفاديا للتلوث و نقلت إلى المختبر في حافظات حرارية خاصة لحين إجراء الاختبارات الضرورية عليها. عوملت العينات الصلبة بالهاضمة لمجانسة كل عينة على انفراد، أما العينات السائلة فقد أخضعت للاختبارات مباشرة بعد إجراء التمديدات عليها. بعد ذلك أجريت الاختبارات البيوكيميائية لمعرفة نوع الجرثومة المتواجدة فيها.
Twenty plant and animal food samples were collected from different commercial stores. These samples were placed in a sterilezed plastic bags not to be polluted and were moved to the laboratory in special thermal containers to be tested. The solid samples were processed in the Digester to study each sample separately , while the liquid samples were tested immediately after being diluted. After that they were studied with biochemical tests to identify the type of germ that existed.
References used
Singleton P (1999). Bacteria in Biology, Biotechnology and Medicine (5th ed.). Wiley. pp. 444–454. ISBN 0-471-98880-4
Escherichia coli". CDC National Center for Emerging and Zoonotic Infectious Diseases. Retrieved 2012-10-02
gt RL, Dippold L (2005). "Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002". Public Health Reports. 120 (2): 174–8. PMC 1497708. PMID 15842119
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204 samples were collected from various body samples
during the year 2016. These 204 samples distributed as
follows: 133 from urine , 37 swabs of wounds and burns,
10 swabs from the ear and pharynx, 12 from feces, 8
from CSF, 4 from blood. This s
tudy distinctly showed that
gram negative bacilli were the most isolates 136 (67%)
while 68 isolates (33%) were gram positive bacteria .
E. coli dominated (39.68%) of the total gram negative
bacilli.
This study distinctly showed an increase in the ratio of
E. coli resistance to one or more types of antibiotics , we
found that E. coli resistance to all antibiotics except
specific and expensive antibiotics, and we found that its
complete resistance to the second ,third and fourth
generation of cephalosporins , resistance to specific and
antibiotics such as Imipenem.
This research was conducted estimate total count of bacteria, and the census total coliform group, fecal coliforms, Escherichia coli .
يتضمن هذا البحث جمع عشرون عينة غذائية من منشأ نباتي وحيواني مختلفة المصدر من الأسواق التجارية , و وضعها ضمن أكياس نايلون معقمة تفادياً للتلوث ونقلها للمختبر
The bacterial case of groundwater samples from Homs
Governorate was studied to determine the bacterial load,
Twenty-eight groundwater samples were collected from
various areas (including areas of the western, northern and
eastern governorates of
the governorate) between March and
July 2017. Where they were collected in sterile glass
containers after opening and running the water for 5 minutes
to avoid any external contamination.
Two hundred and thirty-six E. coli isolates n=236 were collected. Antibiotic susceptibility and ESBL production were studied by disc diffusion and DDST (double disk synergy tests) according to Clinical Laboratory Standards Institute guidelines (CLSI)
and EUCAST for ESBL.
75 (32%)of total isolates demonstrated MDR (multi drug resistance),62 of resistant bacteria (26% of total) were determined as ESBL producers.
In conclusion, we recommend adding this standard, cheap ESBL detection method to the routine microbial susceptibility tests run in Syrian hospitals in order to determine and isolate the sources of MDR infections and do some effort to contain and eliminate those strains.
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