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Register a new userThe ability of some local fungal isolates in enzyme lipase production and determine optimal growth conditions for its production By Penisillium devirsum
قدرة بعض العزلات الفطرية المحلية على إنتاج أنزيم الليباز و تحديد الظروف المثلى لإنتاجه من Penicilliumdevirsum
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Tishreen University ورقة بحثية
Publication date
2016
fields
Biology
and research's language is
العربية
Authors
سناء سارة( باحث )
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Shamra Editor
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تمّ الكشف عن قابلية العزلات الفطريــــــة المحليــــة الآتية:Aspergillus niger،Alternariaalternata، Fusariumoxysporum ، Fusarium solani و Penicilliumdevirsum، على إنتاج أنزيم الليباز باستخدام الوسط الصلب المحتوي على مادة Triputyrin. تميّز الفطر P.devirsum بنشاطه الأنزيمي على بقية الفطور، بينما فشل الفطر A.alternata في إنتاج الليباز. ثمّ أجريّ اختبار كمي باستخدام الوسط السائل لتحديد الظروف المثلى (المصدر الكربوني و تركيزه، فترة الحضن، درجة الحرارة، pH الوسط، المصدر النيتروجيني) لأفضل نمو للفطر P. devirsum و لأفضل إنتاج لأنزيم الليباز. فتبين أنّ زيت الذرة هو الأفضل كمصدر كربوني لنمو الفطر بعد 5 أيام من الحضن، إذ بلغت الكتلة الحيوية (15.99 g/L) و بلغت فعالية الليباز (67.43%) . و قد حقق زيت الذرة بتركيز 2% أفضل كتلة حيوية (17.83g/L) و أفضل فعالية لأنزيم الليباز (72.78%). و حققت درجة الحرارة 34C أعلى كتلة حيوية (18.94 g/L) و أفضل فعالية للأنزيم (76.16%)، و كانت pH=7.0 هي المثلى لنمو الفطر بكتلة حيوية (21.87 g/L) و بفعالية أنزيمية (82.93%). في حين أعطى المصدر النتروجيني ببتون أكبر قيمة للكتلة الحيوية (27.08 g/L) و أقصى فعالية للأنزيم (88.12%)، و ذلك في الشروط المثلى التي تمّ التوصّل إليها في هذه الدراسة.
It wasdetected for the ability of a local fungus A.niger, A.alternata, F.oxysporum, F.solaniandP.devirsum to produce the enzyme lipase in solidmedium including Triputyrin. The enzymic activity of P.devirsum fungus is distinction from other fungus, while the fungus A.alternata failed to produce the lipase. Then they tested quantitatively using a liquid center to determine the best conditions for best growth to fungus P.devirsum and for best produce to enzyme lipase. It indicates that corn oil is the best carbonic source for growth of fungus after 5 days of incubation, as biomass reached(15.99g/L) and effective of lipase (67.43%).And it found that the concentration of 2% of the corn oil has achieved best biomass (17.83g/L) and best production of enzyme lipase effectively(72.78%). Temperature 34 c achieved the highest biomass (18.94g/L) and best production of enzyme lipase (76.16%), and pH =7 was the best for the growth of fungus in biomass reached (21.87g/L) and production of lipase effectively (82.93%). While the nitrogenic source gave peptone biggest value to biomass (27.08 g/L) and highest production for enzyme (88.12%) in optimal conditions reached.
References used
SNELLUMAN,EA. and COLWELL,RR. Acinetobacter lipase; molecular biology, biochemical properties and piotechnological potential. J. Industrial Microbiol and Biotechnol, 2004, 31(9): 391-400
LIMA,V.M.G.; KRIEGER, N.; SARQUIS, M.I.M.; MITCHELL, D.A.; RAMOS, L.P. and FONTANA,J.D. Effect of nitrogen and carbon sources on lipase production by Penicilliumaurantiogriseum. Foot Technol. Biotechnol.,2003, 41(20:105- 110
JAEGER,K.E.; LIEBETON, K.; ZONTA, A. SCHIMOSSEK,K. and REETZ,M.T. Biotechnologecal application of Pseudomonas aeruginosa lipase: efficient kinetic resolution of amines and alcohols. APPL. Microb. Biotechnol.,1996, 46: 99-105
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The following Fungal species were isolated from therhizosphere of wheat plant (TriticumaestivumL.): Trichodermaharzianum, T. viride, T. longibrachiatum, Rhizoctoniasolani, Alternariaalternata, Fusariumsolani, Fusariumoxysporum and Aspergillusniger. I
t was shown that all isolated fungi had the capacity to produce cellulase in solid medium (CMC- Agar), while the fungus T.viride gave the highest capability for the production of this enzyme(5.16). The fungus Rhizoctoniasolani gave less productive ratio (2.61). Quantitative test using Mandelium liquid medium showed that the fungus T.viride had the highest productivity of Cellulase(4.39 U/mL), and this fungus had greater biomass (8.96 g/L). The fungus Rhizoctoniasolani gave lower enzyme productivity(2.04 U/mL) and its biomass reached(4.65 g/L). The study of different carbon sources for the fungus T.harzianum showed that Sucrose was the best media in Cellulase productivity. It reached (3.87U/mL), and the biomass was (2.83 g/L).
It was detected for the ability of a local fungus T.harzianum and A.alternate which
was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the
enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that
T.harzianum and A.alternate have an ability to produce this enzyme, While the
T.harzianum have higher production of cellulase than A.alternate. Then conducted a
quantitative test using Mandelium liquid medium is used to determine the optimal
conditions (incubation time, temperature, PH) for the best cellulase production of the two
fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days
inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While
the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL),
temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the
enzymatic activity average for both fungus , was showed superiority T.harzianum by
production cellulase than fungus A.alternate. And discern that the effect of the incubation
time on cellulase production from fungus T.harzianum more than its effect on cellulase
production from fungus A.alternate. While effect of pH and temperature on cellulase
production from fungus T.harzianum less than their effect on cellulase production from
fungus A.alternate.
Ten isolates of fungi-producing amylase were isolated from six different
sources (different kinds of soils - air-spoilage bread) on malt extract agar
(MEA) and potato dextrose agar (PDA). Four isolates were identified in
Museum of Natural History
Paris- France and six isolates were identified in the
College of Agriculture, Damascus University.
The ability of fungal isolates for secretion aflatoxins B1, B2 and G1 was
detected by thin layer chromatography technique and the quantity of toxins
was determined by using electronic scanner in Syrian Atomic Agency by
comparison with similar standard toxins.
The Mediterranean region is world’s leading olive growing area. A large amount of
liquid waste results from olive oil extraction these wastewaters have a different effects an
environmental . In this study, four fungal isolates from Samples of soil
Polluted by olive
mill wastewater ,this isolates follow to several types from deuteromycetes, they are follow
to this types : Humicola sp., paecilomyces javanicus, Aspergillus terreus, Aspergillus
flavus. They isolated them on tow media (PDA, CzA) and identify them on colony
character ,Biometric and compare them with reference studies.
Forty Isolates of Streptomyces bacteria were obtained from soil samples
from different regions of Syria (Damascus, Sweida, Daraa, Homs, Latakia,
Damascus, Hasakeh, Deir-ezzor) during 2012. The isolates were identified using
ISP (International Stre
ptomyces Project) methodology based on morphological
and physiological criteria. Isolates were grown on selective solid media
depending on growth density as an indicator of the consumption of xylose as an
unique carbon source then on liquid media to determine their ability to
produce the glucose isomerase enzyme. The crude enzyme was extracted using
CTAB 0.1% solution, the enzyme activity was estimated using glucose as a
substrate and the end product was measured colorimetrically at 560 nm.
Results showed that the 40 isolates belong to 29 different species of
Streptomyces. All isolates were able to produce glucose isomerase but they
varied in their efficiency. Isolate SH10 (S. roseiscleroticus) was distinguished by
its high level of enzyme production reaching 4.9 units /ml.
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