A comparison of the optimal conditions for the cellulase production by local fungal isolates T.harzianum and A.alternate

It was detected for the ability of a local fungus T.harzianum and A.alternate which was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that T.harzianum and A.alternate have an ability to produce this enzyme, While the T.harzianum have higher production of cellulase than A.alternate. Then conducted a quantitative test using Mandelium liquid medium is used to determine the optimal conditions (incubation time, temperature, PH) for the best cellulase production of the two fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL), temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the enzymatic activity average for both fungus , was showed superiority T.harzianum by production cellulase than fungus A.alternate. And discern that the effect of the incubation time on cellulase production from fungus T.harzianum more than its effect on cellulase production from fungus A.alternate. While effect of pH and temperature on cellulase production from fungus T.harzianum less than their effect on cellulase production from fungus A.alternate.

Study of antagonistic capability of liquid culture filtrate of isolate of Trichoderma harzianum against some plant and human pathogenic fungi

The antagonistic activity of liquid culture filtrate of Trichodermaharzianum at different concentrations (5, 10, 20 and 30%) was evaluated "in vitro" at 25 C° against following plant and human pathogenic fungi: Aspergillusniger, Rhizopusstolonifer, Fusariumoxysporum, F.moniliforme, Alternariaalternata and Candida albicans. The results showed high level activity of culture filtrate inhibiting the growth of tested fungi. The activity was varied with the different species of fungi and the different concentrations of culture filtrate. The highest activity was against fungus A.niger which showed precent inhibition equal 96.3% at 30% concentration. Whereas the lowest inhibition percentage to 77.6% was recorded for fungus A.alternata in comparison with other fungi. The culture filterate was not affected the radial colonies growth of R.stolonifer at 5% and 10% concentrations, however the colonies was fragile and weak, and clearly inhibited the spores formation. The culture filtrate was showed high antagonistic capability against the human pathogenic fungus C.albicans, and the radial inhibition growth was 1.38 cm at 30% concentration.