تمّ الكشف عن قابلية العزلتين الفطريتين المحليتين Trichoderma harzianum و Alternaria alternate، المعزولتين من المحيط الجذري لنبات القمح Triticum aestivum L.، لإنتاج أنزيم السيلّولاز في الأوساط الصلبة CMC-Agar. فلوحظ أنّ للفطرين قابلية لإنتاج الأنزيم، و تفوق الفطر T.harzianum في إنتاجه للسيلّولاز على الفطر A.alternate. ثمّ أجريّ اختبار كمي باستخدام وسط مندل السائل لتحديد الظروف المثلى من (فترة حضن، درجة حرارة و pH) لأفضل إنتاج للسيلّولاز من الفطرين. فكانت أفضل ظروف لإنتاجه من الفطر T.harzianum هي 7 أيام حضن (3.97 U/mL) ، درجة حرارة 26C (4.07 U/mL) و pH=5.5 (4.19U/mL)، بينما أفضل ظروف لإنتاج السيلّولاز من الفطر A.alternate هي 8 أيام حضن (3.29U/mL)، درجة حرارة 28C (3.15 U/mL) و pH=5.0 (3.86 U/mL). و تبين من المقارنة بين متوسطات الفعالية الأنزيمية لكلا الفطرين تفوّق الفطر T.harzianum بإنتاجه للسيلّولاز على الفطر A.alternate، كما و تبين أنّ تأثير زمن الحضن على إنتاج السيلّولاز من الفطر T.harzianum أكبر من تأثيره على إنتاجه من الفطر A.alternate، في حين تأثير كل من درجة الحرارة و درجة pH على إنتاج السيلّولاز من الفطر T.harzianum أقل من تأثيرهما على إنتاجه من الفطر A.alternate.
It was detected for the ability of a local fungus T.harzianum and A.alternate which
was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the
enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that
T.harzianum and A.alternate have an ability to produce this enzyme, While the
T.harzianum have higher production of cellulase than A.alternate. Then conducted a
quantitative test using Mandelium liquid medium is used to determine the optimal
conditions (incubation time, temperature, PH) for the best cellulase production of the two
fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days
inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While
the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL),
temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the
enzymatic activity average for both fungus , was showed superiority T.harzianum by
production cellulase than fungus A.alternate. And discern that the effect of the incubation
time on cellulase production from fungus T.harzianum more than its effect on cellulase
production from fungus A.alternate. While effect of pH and temperature on cellulase
production from fungus T.harzianum less than their effect on cellulase production from
fungus A.alternate.
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It wasdetected for the ability of a local fungus A.niger, A.alternata, F.oxysporum,
F.solaniandP.devirsum to produce the enzyme lipase in solidmedium including Triputyrin.
The enzymic activity of P.devirsum fungus is distinction from other fungus,
The following Fungal species were isolated from therhizosphere of wheat plant (TriticumaestivumL.): Trichodermaharzianum, T. viride, T. longibrachiatum, Rhizoctoniasolani, Alternariaalternata, Fusariumsolani, Fusariumoxysporum and Aspergillusniger. I
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
45 samples were collected from various sources (soil, degraded wood and
mushroom compost) from three cities (Damascus, Homs and Lattakia). 18
Trichoderma isolates were isolated and identified by microscope. These isolates
were screened using CMC m
Tylosin and Spiramycin are medium-spectrum macrolide antibiotic used exclusively in veterinary medicine for the treatment of a wide range of infections.This research deals with the determination of optimal conditions for simultaneous separation and d