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Register a new userIsolation And Cloning Of AapJ Gene From Brucella melitensis
عزل مورثة ال AapJ و تنسيلها من بكتريا البروسيلا الضأنية
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Damascus University ورقة بحثية
Publication date
2013
fields
Biology
and research's language is
العربية
Created by
Shamra Editor
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تعد البروسيلا بكتيريا ممرضة مشتركة بين الإنسان و الحيوانات الأهلية، و قد تزايدت الخسائر الاقتصادية الناجمة عن تأثير البروسيلا في الثروة الحيوانية التي تنعكس بدورها على الإنسان، و هي بذلك تمثل ضرراً للصحة و الاقتصاد في سورية. و قد تركزت الجهود للوقاية من الإصابة بداء البروسيلا من خلال اللجوء إلى استخدام لقاحات محضرة من سلالات البروسيلا، يجري العمل حالياً لتحضير بروتينات مؤشبة يمكن استخدامها لقاحاً أو محسناً للقاح تقليدي، و ذلك من خلال دراسة المورثات المرمزة للبروتينات المسؤولة عن الفوعة عند البروسيلا عن طريق تنسيلها.
Economic losses resulting from the impact of brucellosis on animals have increased, which is reflected in turn on humans. Brucella is pathogenic bacteria shared by humans and pet animals. They still pose a significant risk that threatens health and economics in Syria. Many efforts have focused to prevent the spread of brucellosis disease, by resorting to the use of vaccines prepared from Brucella strains, where now the target is preparation of vaccine components proteins of Brucella can be used as a vaccine or an improved traditional vaccine.
References used
Al-Mariri, A, 2010. Protection of BALB/c mice against Brucella melitensis 16M infection induced by vaccination with live Escherchia coli expression Brucella P39 protein. Vaccine 28
Ana p. Teixeira-Gomes, Axel Cloeckaert, and ichel S. Zygmunt, 2000. Characterization of Heat, Oxidative, and Acid Stress Responses in Brucella melitensis. Laboratoire de Pathologie Infectieuseet Immunologie, Institut National de la Recherche
Delrue, R. M., Lestrate, P., Tibor, A., Letesson, J. J and De Bolle X, 2004. Brucella pathogenesis genes identified from random large-scale screens FEMS microbiology letters
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Isolation and identification of Pseudomonas aeruginosa was screened from
15 samples of Syrian oily-soils to determine the ability to produce Lipase
enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during
2007-2008 AD. The results sho
wed that there were grown colonies with color of
green or yellow-green surrounded with different diameter of clear zone in three
samples and the identification by Mowasat Hospital revealed that it was
Pseudomonas aeruginosa In addition, the results showed that the optimum
conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of
production of lipase with highest activity of 63.3% was three days in
submerged culture during the optimum conditions. These results illustrated the
important of this isolates to produce and control lipase for industrial
production.
Samples from carrot and lettuce roots, sclerotia of Sclerotinia sclerotiorum,
and soil were collected from south of Damascus during 2001. 82 isolates were
isolated, 48 of them were isolated from roots, 27 from sarfuce of sclerotia, and
7 from soil
around roots. Antgonisim capacity of these isolates was tested
against S. Sclerotiorum in vitro. 7 bacterial isolates showed antagonistic effect
on the growth of S. Sclerotiorum, the antagonism distance ranged bettwen (1.3 –
11 mm). The Isolate (K82.4), which was isolated from soil, showed antagonistic
distance (11mm), and was the best of bacterial isolates. Three isolates were
iddentified (K82.4 isolated from lettuce soil, FN3 isolated from surface of
sclerotia, K4.4 isolated from carrot roots), that belong to Bacillus spp.
Brucellosis is an endemic zoonosis in Syria, affecting large numbers of
animals and an increasing number of humans. Brucella is an intracellular
pathogen capable of infecting animals and humans. The aim of this study was
to develop a polymerase ch
ain reaction (PCR) protocol for the detection of
Brucella melitensis in artificially contaminated cheese.
This study were conducted on 450 samples of raw chicken meat
( 150 samples of thighs- 150 samples of breasts -150 samples of
wings ) were collected from retail marketin Hama city,to detect
contamination of staphylococcus.
Enterobacter sakazakii is considered an opportunistic pathogen that has
been associated with severe lethal infections especially in neonates, elderly, and
Immunocompromised adults. E. sakazakii is a Gram negative, facultative
anaerobes rod-shaped
bacterium. It belongs to the family Enterobacteriaceae
and genus Enterobacter.
Although we don’t know the natural habitat of this bacteria we find that it
exists in high rate in herbs and spices which indicates that plant may be this
natural habitat.
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