Two bacterial species were isolated from petroleum hydrocarbon-contaminated soil.
The isolates were characterized based on their gram reaction characteristics, morphological
and biochemical properties.
The results showed two bacteria isolated the
first isolated: gram negative short rod,
urease and nitrate reduction test showed a positive result , and glucose test showed positive
result.
And the second isolated: gram positive cocci, gelatin and Citrate utilization test
showed a positive result , and maltose, mannitol test showed negative result.
They were then incubated at (37) ° C for (48- 72) hours.
This is based on comparison of results with Bergey Manual of systematic
bacteriology.
The isolates were identified to be: Pseudomonas pachastrellae (X1)
Micrococcus brunensis (X2).
