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Register a new userStudy of antagonistic capability of liquid culture filtrate of isolate of Trichoderma harzianum against some plant and human pathogenic fungi
دراسة القدرة التضادية للرشاحة السائلة لعزلة من الفطر Trichodermaharzianumتجاه بعض الفطريات الممرضة للنبات و الإنسان
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ميساء يازجي( باحث )
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اختبرت القدرة التضادية للرشاحة السائلة للفطر Trichodermaharzianum بتراكيز مختلفة (5، 10، 20 و 30%) تجاه الفطريات الممرضة للنبات و الإنسان التالية: Aspergillusniger، Rhizopusstolonifer، Fusariumoxysporum،F.moniliforme، Alternariaalternata و Candida albicans ضمن الشروط المخبرية بالدرجة 25 مْ. بيّنت النتائج فعاليّة عالية للرشاحة في تثبيط نمو الفطريات المدروسة. اختلفت هذه الفعاليّة باختلاف الأنواع الفطريّة و اختلاف التراكيز المستخدمة من الرشاحة، و كانت الفعالية الأكبر تجاه الفطر A. niger الذي أظهر نسبة تثبيط وصلت إلى 96.3% بالتركيز 30%، في حين سجلت النسبة الأدنى للتثبيط و التي وصلت إلى 77.6% مع الفطر A. Alternate مقارنة بالفطريات الأخرى. لم تؤثّر الرشاحة في قطر مستعمرات الفطر R. stolonifer بالتركيزين 5 و 10% لكنها كانت مستعمرات هشّة و ضعيفة و ثبّطت التبوغ بشكل واضح. بيّنت الرشاحة قدرة تضاديّة جيّدة تجاه الفطر الممرض للإنسان C. Albicans و قد وصل قطر تثبيط النمو إلى 1.38 سم بالتركيز 30%.
The antagonistic activity of liquid culture filtrate of Trichodermaharzianum at different concentrations (5, 10, 20 and 30%) was evaluated "in vitro" at 25 C° against following plant and human pathogenic fungi: Aspergillusniger, Rhizopusstolonifer, Fusariumoxysporum, F.moniliforme, Alternariaalternata and Candida albicans. The results showed high level activity of culture filtrate inhibiting the growth of tested fungi. The activity was varied with the different species of fungi and the different concentrations of culture filtrate. The highest activity was against fungus A.niger which showed precent inhibition equal 96.3% at 30% concentration. Whereas the lowest inhibition percentage to 77.6% was recorded for fungus A.alternata in comparison with other fungi. The culture filterate was not affected the radial colonies growth of R.stolonifer at 5% and 10% concentrations, however the colonies was fragile and weak, and clearly inhibited the spores formation. The culture filtrate was showed high antagonistic capability against the human pathogenic fungus C.albicans, and the radial inhibition growth was 1.38 cm at 30% concentration.
References used
ABIALA,M.A.;OGUNJOBI,A.A.; ODEBODE, A.c. and AYODELE,A.C. and AYODELE, M.A. Microbial control of MycosphaerellafijiensisMorelet a notable pathogen of Bananas and plantains. Nature and science, Vol.8, No.10, 2010, 299- 305
ABU-TALEB,A.M. and AL-MOUSA,A.A. Evaluation of antifungual activity of vitavax and Trichodermaviride against two wheat root rot pathogens. Journal of Applied Bioscences, Vol.6, 2008, 140-149
ADEBESIN,A.A.; ODEBODE,C.A. and AYODELE,A.M. Control of postharvest rots of Banana fruits by conidia and culture filtrates of Trichodermaasperellum. Journal of plant protection research, Vol.49, No.3, 2009, 302-308
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