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Detection of Antibacterial Effect of Lactic Acid Bacteria Isolated

التحري عن الفعالية المضادة لنمو البكتيريا لعزلات بكتيريا حمض اللبن معزولة من بعض الأغذية السورية

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 Publication date 2018
  fields Food Sciences
and research's language is العربية
 Created by Shamra Editor




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Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.

References used
Hassanzadazar, H., and Ehsani, A. (2013). Phenotypic Characterization of Lactic Acid Bacteria Isolated from Traditional Koopeh Cheese. Global Veterinaria. Vol. 10 (2): 148-152
Ishola, R. O., and Adebayo-Tayo, B. C. (2012). Screening of Lactic Acid Bacteria Isolated from Fermented Food for Bio-molecules Production. AU J.T. Vol. 15 (4): 205-217
Moghadam, M. Sh., Foo, H. L., Leow, T. C., Abdul Rahim, R. and Loh, T. Ch. (2010). Novel Bacteriocinogenic Lactobacillus plantarum Strains and Their Differentiation by Sequence Analysis of 16S rDNA,16S-23S and 23S-5S Intergenic Spacer Regions and Randomly Amplified Polymorphic DNA Analysis. Food Technol. Biotechnol. Vol. 48 (4): 476–483
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This study was conducted at the laboratory of Food Science Department, Agriculture College, Damascus University to isolate and characterize of Lactobacilli bacteria from some Syrian foods and detection the effectiveness of anti-pathogenic bacteria l growth. Lactic acid bacteria from different dairy products, pickles and dried plant samples were isolated and identified according to common characteristics. Results showed that twenty-three isolates were rods while only 2 of the them were coccid. These isolates were tested for their antimicrobial effect against pathogenic bacteria for both gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial effect against the three tested bacteria, of which CP50 and CP46 isolates showed the best antimicrobial effect. Results of the standard physiological and biochemical tests identified fourteen isolates as Lactobacillus plantarum and one isolate as Lactobacillus fermentum.
This research was conducted in the Agriculture Faculty- Food Science Department, Damascus University, for detecting Lactic acid bacteria isolated from Syrian dairy products (white fresh cheese-Leben), Samples were collected from August, 2004 to Oc tober, 2005 from different areas in Syria. Analysis was done for 245 bacterial isolates from 40 samples of white fresh cheese and 58 samples of yogurt (curd). Results showed that the genus Enterococcus was dominant with a percentage of 47.52%; Pediococcus had a percentage of 26.3 %; while Lactococcus had a percentage of 15.38% with only 3.2% for Lactobacillus.
Thirty strains of lactic acid bacteria were isolated from different local fermented foods. Isolates were identified by using morphological, cultural and biochemical tests using the API 50-CHL and API 20 Strep systems. Preliminary characterization tests showed that 33.33% of the isolates were rod shaped and 66.67% belonged to cocci bacteria.
This research was conducted at the Faculty of Agriculture, Department of Food Science laboratories to isolate lactic acid bacteria from some Syrian milk products (milk, yogurt, cheese) and determine some morphological, physiological characteristic and the ability of these isolates to ferment sugars.
Fifty eight isolates of lactic acid bacteria were obtained from different local fermented foods. Isolates were identified using microscopic, cultural and biochemical tests using API strips. Results showed that 44.83% of the isolates were rod shape d and 55.17% belonged to cocci bacteria.
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