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Register a new userFactors affecting leaf protoplast isolation from Binella -potato cultivar
العوامل المؤثرة في عزل البروتوبلاست الورقية في صنف البطاطا بينيلا
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عزل البروتوبلاست من أوراق صنف البطاطا بينيلا في مخابر كلية الزراعة- جامعـة دمـشق بهـدف تحديد العوامل المؤثرة في مردود و حيوية البروتوبلاست. أظهرت النتائج إمكانية الحـصول علـى أكبـر مردود من البروتوبلاست المعزول.
In this research, protoplast isolations were obtained from the leaves of Potato Binella cultivar at the laboratories of Agriculture College- Damascus University in order to determine factors affecting the yield and viability of protoplast isolation. Results indicated that the best yield percentage of isolated protoplast.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة العوامل المؤثرة في عزل البروتوبلاست من أوراق صنف البطاطا بينيلا في مخابر كلية الزراعة بجامعة دمشق. هدفت الدراسة إلى تحديد العوامل التي تؤثر على مردود وحيوية البروتوبلاست المعزول. أظهرت النتائج أن استخدام ثيوسلفات الفضة (STS) بتركيز 50 ميكرومول أدى إلى الحصول على أعلى مردود من البروتوبلاست (1.7 ± 0.06 × 10^6 بروتوبلاست/غ أوراق) مع نسبة حيوية مرتفعة (82.91 ± 2.38%). كما أظهرت الدراسة أن إضافة المانيتول بتركيز 0.75 مول إلى وسط العزل أعطى نتائج أفضل من السكروز، حيث بلغ مردود البروتوبلاست (1.68 ± 0.04 × 10^6 بروتوبلاست/غ أوراق) ونسبة حيوية (87.82 ± 2.64%). كما تبين أن استخدام أوراق نباتات مزروعة مخبرياً بعمر 4 أسابيع أدى إلى الحصول على أعلى مردود وحيوية من البروتوبلاست المعزول بعد فترة حضانة (14-16) ساعة في محلول إنزيمي محدد.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال تحسين النباتات باستخدام تقنية عزل البروتوبلاست، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن توسيع نطاق الدراسة ليشمل أصناف أخرى من البطاطا أو نباتات أخرى لمعرفة مدى تطبيق النتائج على نطاق أوسع. ثانياً، لم يتم التطرق بشكل كافٍ إلى تأثير العوامل البيئية مثل درجة الحرارة والرطوبة على عملية العزل. أخيراً، كان من الممكن تقديم تفاصيل أكثر حول التحديات التي واجهت الباحثين أثناء التجارب وكيف تم التغلب عليها.
Questions related to the research
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ما هو التركيز الأمثل لثيوسلفات الفضة للحصول على أعلى مردود من البروتوبلاست؟
التركيز الأمثل لثيوسلفات الفضة هو 50 ميكرومول للحصول على أعلى مردود من البروتوبلاست (1.7 ± 0.06 × 10^6 بروتوبلاست/غ أوراق).
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ما هي النسبة المئوية للحيوية عند استخدام المانيتول بتركيز 0.75 مول؟
النسبة المئوية للحيوية عند استخدام المانيتول بتركيز 0.75 مول هي 87.82 ± 2.64%.
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ما هو العمر المثالي للأوراق للحصول على أعلى مردود من البروتوبلاست المعزول؟
العمر المثالي للأوراق للحصول على أعلى مردود من البروتوبلاست المعزول هو 4 أسابيع.
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ما هي الإنزيمات المستخدمة في محلول العزل الإنزيمي؟
الإنزيمات المستخدمة في محلول العزل الإنزيمي هي 1.75% سيلوليز و 0.4% ماسيروزيم.
References used
Assani, A., R. Haicour, G. Wenzel, W. B. Foroughi, F. Bakry, F.X. Cote, G. Ducreux, A. Ambroise and A. Grapin. 2001. Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci., 162: 355-362
Avihai P., D. Aviv and E. Galun. 1988. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.Plant Cell Rep., 7:403- 406
Babaoglu, M. 2000. Protoplast isolation in Lupin (Lupinus mutabilis: sweet) and determination of optimum expplant sources and isolation conditions. Turk. J. Botanical, 24: 177-185
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