Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userFactors affecting leaf protoplast isolation from Binella -potato cultivar
العوامل المؤثرة في عزل البروتوبلاست الورقية في صنف البطاطا بينيلا
1602
0 65
0
(
0
)
Created by
Shamra Editor
Ask ChatGPT about the research
عزل البروتوبلاست من أوراق صنف البطاطا بينيلا في مخابر كلية الزراعة- جامعـة دمـشق بهـدف تحديد العوامل المؤثرة في مردود و حيوية البروتوبلاست. أظهرت النتائج إمكانية الحـصول علـى أكبـر مردود من البروتوبلاست المعزول.
In this research, protoplast isolations were obtained from the leaves of Potato Binella cultivar at the laboratories of Agriculture College- Damascus University in order to determine factors affecting the yield and viability of protoplast isolation. Results indicated that the best yield percentage of isolated protoplast.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة العوامل المؤثرة في عزل البروتوبلاست من أوراق صنف البطاطا بينيلا في مخابر كلية الزراعة بجامعة دمشق. هدفت الدراسة إلى تحديد العوامل التي تؤثر على مردود وحيوية البروتوبلاست المعزول. أظهرت النتائج أن استخدام ثيوسلفات الفضة (STS) بتركيز 50 ميكرومول أدى إلى الحصول على أعلى مردود من البروتوبلاست (1.7 ± 0.06 × 10^6 بروتوبلاست/غ أوراق) مع نسبة حيوية مرتفعة (82.91 ± 2.38%). كما أظهرت الدراسة أن إضافة المانيتول بتركيز 0.75 مول إلى وسط العزل أعطى نتائج أفضل من السكروز، حيث بلغ مردود البروتوبلاست (1.68 ± 0.04 × 10^6 بروتوبلاست/غ أوراق) ونسبة حيوية (87.82 ± 2.64%). كما تبين أن استخدام أوراق نباتات مزروعة مخبرياً بعمر 4 أسابيع أدى إلى الحصول على أعلى مردود وحيوية من البروتوبلاست المعزول بعد فترة حضانة (14-16) ساعة في محلول إنزيمي محدد.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال تحسين النباتات باستخدام تقنية عزل البروتوبلاست، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن توسيع نطاق الدراسة ليشمل أصناف أخرى من البطاطا أو نباتات أخرى لمعرفة مدى تطبيق النتائج على نطاق أوسع. ثانياً، لم يتم التطرق بشكل كافٍ إلى تأثير العوامل البيئية مثل درجة الحرارة والرطوبة على عملية العزل. أخيراً، كان من الممكن تقديم تفاصيل أكثر حول التحديات التي واجهت الباحثين أثناء التجارب وكيف تم التغلب عليها.
Questions related to the research
-
ما هو التركيز الأمثل لثيوسلفات الفضة للحصول على أعلى مردود من البروتوبلاست؟
التركيز الأمثل لثيوسلفات الفضة هو 50 ميكرومول للحصول على أعلى مردود من البروتوبلاست (1.7 ± 0.06 × 10^6 بروتوبلاست/غ أوراق).
-
ما هي النسبة المئوية للحيوية عند استخدام المانيتول بتركيز 0.75 مول؟
النسبة المئوية للحيوية عند استخدام المانيتول بتركيز 0.75 مول هي 87.82 ± 2.64%.
-
ما هو العمر المثالي للأوراق للحصول على أعلى مردود من البروتوبلاست المعزول؟
العمر المثالي للأوراق للحصول على أعلى مردود من البروتوبلاست المعزول هو 4 أسابيع.
-
ما هي الإنزيمات المستخدمة في محلول العزل الإنزيمي؟
الإنزيمات المستخدمة في محلول العزل الإنزيمي هي 1.75% سيلوليز و 0.4% ماسيروزيم.
References used
Assani, A., R. Haicour, G. Wenzel, W. B. Foroughi, F. Bakry, F.X. Cote, G. Ducreux, A. Ambroise and A. Grapin. 2001. Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci., 162: 355-362
Avihai P., D. Aviv and E. Galun. 1988. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.Plant Cell Rep., 7:403- 406
Babaoglu, M. 2000. Protoplast isolation in Lupin (Lupinus mutabilis: sweet) and determination of optimum expplant sources and isolation conditions. Turk. J. Botanical, 24: 177-185
rate research
Read More
Potato (Solanum tuberosum L.) is among the most
economically important crops world-wide and in Syria
as well. It is highly responsive to different tissue culture
techniques.
In vitro culture of potato cv. 'Draga' (mid-early) was
established thro
ugh meristem tip culture.
This research studies the competitiveness ability of Syrian exports and the major factors empowering this ability, the availability of these factors considers one of the most important elements to ensure maximum productivity and best use of resources
, and thus reach higher rates of economic growth, taking into account the importance of analyzing the excellence elements which Syrian Exportation Sector owns in light of all restrictive factors facing
competitiveness ability including production costs quality and government role, this research identified a group of remarks and conclusions indicating that although Syria owns market power elements but they are not invested in a sufficient way.
Therefore, the Syrian market is weak with respect to the competitiveness ability, in addition to a clear weakness of the competitiveness of the Syrian exportations, in particular the industrial exportations. This results in the need to implement a group of recommendations or solutions in order to overcome this dilemma. The forefront of these recommendations is the need to support the Syrian exportation’s sectors that have the best competitiveness ability to compete in a clear and transparent
framework of strategy for external trade.
Development of the nursing profession and address future shortages in the labor force depends largely on the ability to attract young people to enter it, to attract these young people, it should start to identify the important factors that influence
their choice of nursing profession. The study aims to identify factors influencing choice of students for the nursing profession. Research was conducted on a sample of 250 students(first-year) in Nursing College atTishreen University. Descriptive research methodologywas followed and questionnaire to collect data was used. Most of student perceived nursing as humanitarian job, 60% of them selected nursing as their first choice. For most students (72.8%), the main reason they chose to study nursing is the desire to help others. They were mostly motivated by their parents to join nursing. Nursing College was the first source of information of nursing. Nearly half of the students made the decision to workin nursing after graduationand (40%) decided to pursue higher education,
Poor design is a major factor in reducing the overall performance of construction projects as well as being directly responsible for many projects cost overrun, delay and being plagued with rework,
variations and disputation.
This research discusse
s the main factors affecting Design Quality in the Construction Industry in Syria.
The result is based on a number of interviews and questionnaire surveys involving 54 designers, contractors and construction professionals. The survey identified 45 different factors classified in
categories related to designer, management of the design process, owner and design documents. The severity of those factors was measured by the level of importance and was ranked according to the
importance index.
It was concluded that, insufficient overall design time, method of selecting the designer based on the lowest price offer, lack of documentation and changes client’s requirements were the most important factors. The results show that designers and contractors have different perceptions of the quality of
design factors.
The aim of this study is to assist all construction parties to plan effectively before starting a project during the design phase in order to minimize problems and eliminate extra costs needed to complete
the defective design. Finally, some recommendations were made in order to improve the design quality in the construction industry.
This research was conducted I during 2009-2010 at Dier Alhajar station,
General Commission for Scientific Agricultural Research to determine the
length of calving interval (CI) and the effect of sire, calving year, calving
season, parity and age o
f cow and their interactions in Shami Cattle.
It was concluded that CI can be reduced by better
management and providing good and suitable feeding conditions.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
