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Twelve growing Shami heifers available in Dier-Al-Hajar Station for improving Shami Cattle weighing 115±3 kg and 178±4 days old were used to determine the weekly hormonal concentrations of progesterone (P4) and estradiol 17-β (E2) during the pre- and puberty stages (6-14 mos). Blood samples were collected weekly from the Jugular vein using vacutainer tubes supported with K3EDTA.
Twelve growing Shami heifers available in Dier-Al-Hajar Station for improving Shami Cattle weighing 115±3 kg and 178±4 days old were used to monitor the daily growth of ovarian follicles (daily numbers, size, length and number of waves, rate of gr owth and regression) using Transrectal Ultrasound Echo System (7.5 MHz probe) in 6-14 months-old heifers. Results indicated that ovarian follicles developed in a wave pattern in all Shami heifers when they were 6 months old and no significant differences in the number and size of ovarian follicles were found between right and left ovaries. A cohort of 4 to 7 small follicles with a diameter 2-5 mm developed together and one of them was selected for continual growth, became dominant and later regressed during the pre-pubertal stage.
Thirteen viable and post - frozenthawed Shami cows embryos obtained by super-ovulation and artificial insemination technique in order to determine sex of embryos. Biopsies (1-2 cells) of embryos at morula or blastocyst stage were taken using micro manipulation under inverted microscope. It was concluded that aspiration of 1-2 blastomere (s) from embryos at morula or blastocyst stage was enough to determine sex of embryo using PCR and it is recommended that using this technique can be a useful tool in splitting and transferring of desired sex embryos in genetic improvement program.
This research was conducted I during 2009-2010 at Dier Alhajar station, General Commission for Scientific Agricultural Research to determine the length of calving interval (CI) and the effect of sire, calving year, calving season, parity and age o f cow and their interactions in Shami Cattle. It was concluded that CI can be reduced by better management and providing good and suitable feeding conditions.
Twelve growing Shami heifers available in Dier-Al-Hajar Station for improving Shami Cattle during 2005-2007 weighing 250±11 kg and 400±21 days old were used to monitore the growth of dynamic of ovarian follicles (daily numbers, size, length and nu mber of waves, rate of growth and regression) and characteristics of estrus cycle (length, time of dominancy, diameter of Graffian follicle and estrus and ovulatory times), length of corpus luteum (CL) during the estrous cycle. Heifers were maintained under uniform conditions and fed according to their daily growth. Transrectal Ultrasound Echo System (7.5 MHz probe) was used to study the ovarian structures and to monitor the daily development and regression of ovarian follicles, the formation and regression of corpus luteum in the left and right ovaries during the estrus cycle. Results indicated that the growth turnover of ovarian follicles was observed and developed in a wave pattern in all Shami heifers and no significant differences in the number and sizes of ovarian follicles were found between right and left ovaries. A cohort of 5 to 7 small follicles with a diameter 2-5 mm developed together and one of them was selected to continue growing and becomes dominant and later on being regressed or ovulated during the estrus cycle. One to five follicular waves were observed per cycle and each wave was characterized by the development of one large (dominant) follicle and a variable number of smaller follicles. The estrus cycle with one follicular wave occurred only in heifers exhibited the first post pubertal cycles with an average length equaled to 8.67±0.33 days. Frequency of estrus cycle according to the number of follicular waves was 8.4, 25.3, 44.2, 20 and 2.1% in cycles having 1, 2, 3, 4 and 5 follicular waves, respectively. Furthermore , the length of estrus cycle was also related to the number of follicular waves and it was 21.87±0.62, 22.44±0.74, 26.4±0.72, 26.50±3.50 days in cycles having 2, 3, 4 and 5 follicular waves, respectively with significant differences (P < 0.05) in cycles with 2 or 3 vs. 4 and/or 5 waves. The functional duration of CL was extended to 9, 12 and 15 days in a short (17-19 days), intermediate (20-24 days) and long estrus cycle (≥ 25 days). The maximum diameter of dominant follicles in ovulatory waves was significantly larger (P < 0.05) than that of dominant follicles in nonovulatory waves. In conclusion, this study showed that the development of ovarian follicles in Shami heifers occurs in waves and the most common pattern is three waves per estrous cycle.
This study conducted at Deir Al-Hajar Station during the season 2010-2011 to describe the lactation curve of Shami cattle and determine factors affecting its shape compenents. 1120 records of 356 Shami cows collected between 1997 – 2010 were used. Data were subjected to general linear model, and the analysis of variance used to determine the effect of factors. Duncan test was used to compare the means using SAS system (1996) and Incomplete gamma functions were used to estimate the parameters of lactation curve (a, b and on the basis of daily yield of milk. Results indicated that the average values of the gamma parameters were 2.14 ± 0.01 kg for a (beginning of milking ), 0.61 ± 0.02 kg for b (increasing milk production up to peak) and - 0.23± 0.01 kg for c (decreasing milk production from peak to dry). The effect of calving year was significant (p<0.01) on a, and (p<0.05) on b and (p<0.001) on c. The effect of age at calving and sex of calf was significant (p<0.01) on a but non significant on b and c. Season of calving, parity, and interaction between age and parity was not significant on all parameters. It was concluded that improving the productive performance of Shami cattle, requires applying a long term genetic improvement program to select the best herd.
Fifteen Shami cows available in Dier-Al-Hajar Station for improving Shami Cattle were used during 2009-2010 to evaluate the ovarian response of cows for gonadotropin releasing hormone (GnRH) or human chorionic gonadotropin (hCG) injected on day 5 of the estrus cycle during which dominant follicle is growing in the first follicular wave. Animals were divided randomly into three groups of five cows. Group 1 was left as a control and received no treatment while Groups 2 and 3 received on day 5 of the estrous cycle a single i.m. injection of 8 ?g of a GnRH agonist (Buserelin) and 3000 IU hCG, respectively. Transrectal ovarian ultrasonography was performed daily to monitor ovarian follicular dynamics and corpus luteum.
This study was conducted at the Deir Al-Hajar Shami Cattle Research Station, Animal Wealth Research Administration. For this purpose 12 primiparous Shami cows were used to know the influence of the milking regimes in the production performance of the cow and the composition of the produced milk. The animals were divided randomly into two equal groups. Machine milking were used at the first group in the presence of the calf, or for the machine milking without the presence of the calf at the second group. The daily production of milk was estimated weekly from every cow in both groups and at the same time milk samples were taken to estimate the percentage of the basic components of milk such as fat, protein, lactose and dry matter until the end of lactation. Data were analyzed in statistical program SAS by using variance analysis ANOVA (Mixed Model).
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