Nineteen locally selected potato lines were screened for water stress
tolerance by measuring the growth parameters in vitro. The stress was achieved
by adding 4% of sorbitol to the growth medium. Plant height and diameter,
leaf area, number, lengt
h and diameter of roots and plant fresh and dry weight
were measured. The results showed a difference in the response of the studied
lines to water stress, cluster analysis, based on the sum of all growth
parameters relative values of the lines showed that the lines were divided into
three groups according to water stress tolerance: The tolerant group, the
moderate tolerant group and the sensitive group. The results indicated the
possibility to use the in vitro method to screen the potato lines for water stress
tolerance.
This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorgh
um, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
In this research, protoplast isolations were obtained from the leaves of
Potato Binella cultivar at the laboratories of Agriculture College- Damascus
University in order to determine factors affecting the yield and viability of
protoplast isolation. Results indicated that the best yield percentage of isolated
protoplast.
The research was conducted at Faculty of Agriculture, Damascus University/
Syria, with the help of Scientific Agricultural Research Center in Latakia during
2013 and 2014 seasons. Three rootstocks were used i.e. Sour orange, citrumelo
and troyer c
itrange, to study some factors affecting the success of micrografting
technique (concentrations and periods of sterilization, rootstock, shoot tip size, type
of nutritive medium and the grafting method). The nutritive medium was prepared,
and the shoot tips was isolated from Navel orange source, then the micrografting
was done for the three rootstocks.
أجريت التجارب الخاصة بهذه الدراسة في منطقتين من غوطة دمشق الأولى في منطقة أبي جرش - مزرعة كلية الزراعة والثاني في منطقة خرابو - مزرعة كلية الزراعة
