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The effect of some preservation and storage methods on the microbiological content of capers flower buds (Capparis spinosa L.)

تأثير بعض طرائق الحفظ و التخزين على الحمولة الميكروبيولوجية لبراعم القبّار الزهرية (Capparis spinosa L.)

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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Flower buds of capers were processed by some common methods as pickling, salting, and other uncommon like freezing with and without blanching and canning. The results showed that there was no microbiological growth in all methods since the fourth month of storage. Best growth for lactobacillus bacteria was in 5% brine (8.2 × 106) and then in 8% brine (1.2 × 106)CFU.g-1 . while there wasn’t any growth of this bacteria by using other methods like using high concentration of salt 23%. On other hand, the results showed that this bacteria existed in small account (1.4 × 102) and (1.1 × 102) CFU.g-1 after a week in brine 16% and the freezed sample respectively. There was no statistical difference between the two storage solutions as there wasn̓ t any microbiological growth in both of them through 8 months.


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Research summary
تتناول هذه الدراسة تأثير بعض طرائق الحفظ والتخزين على الحمولة الميكروبيولوجية لبراعم القبار الزهرية (Capparis Spinosa L.). تم تطبيق عدة طرق شائعة مثل التخليل والتمليح، وأخرى غير شائعة مثل التجميد مع وبدون سلق والتعليب. أظهرت النتائج أن جميع طرق التخزين كانت فعالة في القضاء على الحمولة الميكروبيولوجية منذ الشهر الرابع للتخزين. كما أظهرت النتائج أن أفضل نمو للبكتريا اللبنية كان في المحلول الملحي بتركيز 5%، بينما لم يُلاحظ أي نمو للبكتريا في التراكيز الملحية العالية (23%). وأظهرت الدراسة أيضًا أن هناك فرق معنوي بين محلولي التخزين الملحيين من حيث الحمولة الميكروبيولوجية، حيث لم يُلاحظ أي نمو ميكروبي في كليهما طوال فترة التخزين (8 أشهر).
Critical review
تعتبر هذه الدراسة مهمة لأنها تقدم معلومات قيمة حول طرق حفظ وتخزين براعم القبار الزهرية وتأثيرها على الحمولة الميكروبيولوجية. ومع ذلك، يمكن تحسين الدراسة من خلال توسيع نطاق البحث ليشمل تأثير هذه الطرق على القيم الغذائية والنكهة للبراعم. كما يمكن أن تكون هناك حاجة لمزيد من الدراسات حول تأثير إضافة مضافات طبيعية لتحسين جودة المنتج النهائي. بالإضافة إلى ذلك، قد يكون من المفيد دراسة تأثير هذه الطرق على أنواع أخرى من النباتات المشابهة.

References used
ALVARRUIZ, A.; RODRIGO, M., MIQUEL, J.; GINER, V.; FERIA, A. and VIL, R. 1990, Influence of brining and packing conditions on product quality of capers. J Food Sci, 55, 196–227
BARBERA, G. and DI LORENZO, R. 1984, The caper culture in Italy. Acta Hortic, 144, 167–171
BOMRUNGNOK,W.;WONGWICHAM,A.;VONGSAWADI,P. 2007, Effect of Salt Concentration on the Properties of Fermented Green Mustard.The 2ed International Conference of fermentation Technology for Value Added Agriculture Products,Khon Kean,Thailand
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Research was carried out in (24) locations where two subspecies Capparis spinosa were recorded in Syria . The subspecie C. spinosa subsp. rupestris was found in seven locations, mostly in the southern region of the environment, while he found the S yrian C. spinosa subsp. spinosa in seventeen of the study sites in Syria. Chemical analysis showed that, the soils of all location were not salty and pH ranged between (6.9-8.4), Soils texture was clayey and clay loam in most locations. According dependant on equation of Emberger, it was found that distribution of subspecies Capparis spinosa were located in a wide range from bioclimatic very dry to semi- humid zone, through with dry, semi-dry, and Hyper humid zones.
This research was carried out to test the effect of three basic preservation methods cooling, canning and freezing on the nutritional value of mushroom Agaricus bisporus. Mushroom were picked from a private sector then stored for 20 weeks. Chemica l analyses of mushroom were conducted at three phases: at time zero which means immediately after preservation, after ten weeks and after 20 weeks. Sodium benzoate 5% and potassium serrate and 5% were tested as preservatives.
This present study was conducted to develop a detailed in vitro propagation system for the medicinal shrub Capparis spinosa L. Single nodes with one bud and a small part of stem of 1-1.5 cm long were used as initial explants which were collected f rom a shrubs grown under field conditions at Damascus suburb., (Doumar). Explants were surface-disinfected by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100 ml disinfectant solution, where after, they were placed onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the growth room at 23±1 c and light intensity of 3000 lux at the cultures level. Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks on the optimal MS medium (MS+8.88μM BA+0.49μM IBA). The described method has potential to produce large numbers of plantlets within a short period of time to expand its cultivation for medicinal uses.
Samples of Capparis spinosa plant were collected from 6 sites in Aleppo and Lattakia provinces. The genetic variations were studied using the AFLP technique in order to determine the genotypes of the studied types using 3 primers, which showed ampl ification. Statistical analyses were conducted using the dissimilarity coefficient and genetic diversity coefficient. The A.C.P. and the statistical -F were calculated, and the similarity dendrogram was constructed. The results showed the following: The presence of a certain number of specific alleles (descriminates) for each province. The presence of genetic and reproductive isolation deterrent to gene flow between the two provinces. The heterozygoty average was a little higher in Lattakia (0.486) than in Aleppo (0.481). The mean of genetic diversity coefficient of primers and individuals was a little higher in Aleppo (0.677) than in Lattakia (0.653). The population mean was very close: (0.759) in Aleppo and (0.760) in Lattakia. This may be explained on the basis of silent and specific alleles due to deletion mutations, different selective effects as well as the reproduction system in the two cities. The greatest genetic distance in Aleppo was (0.381) noted between (Al Dahea and Al Shekh saaed), and in Lattakia (0.38) noted between (Al Amroniah and Jabla). However, the greatest genetic similarity in Aleppo was (0.637) noted between (Al Shekh Saaed and Turkman Bareh), and in Lattakia (0.675) noted between (Wata Deirzenon and Jabla). The variation between these values was graduated. The study of statistical –F showed the effect of the similarity factor in some populations. This was more significant in Aleppo than in Lattakia, which indicates that the reproduction system is more closed in Aleppo, and this refers as well to inbreeding or self pollination which showed high genetic variations in these populations when compared with Lattakia. The results of this study helped in determining primers that can be used as molecular markers in a breeding program for Capparis spinosa as a medicinal plant. This technique showed high efficiency in studying the similarity relationships between these two cities.
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