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Register a new userStability-indicating HPLC method for pentoxifylline assay in pharmaceuical dosage forms
طريقة كروماتوغرافيا سائلة عالية الأداء مؤشرة للثبات لمقايسة بنتوكسيفيلين في أشكاله الصيدلية
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Tishreen University ورقة بحثية
Publication date
2017
fields
Pharmacy
and research's language is
العربية
Authors
آيات عبود( باحث )
Created by
Shamra Editor
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تصف هذه الدراسة طريقة كروماتوغرافيا سائلة عالية الأداء مؤشرة للثبات لمقايسة بنتوكسيفيلين في أشكاله الصيدليه. جرى فصل المادة الدوائية بنجاح عن نواتج التدرك، بعد تعريضها لشروط اجهاد، باستعمال عمود C18 و طور متحرك مكون من ماء و ميثانول (60: 40، حجم: حجم بتدفق 1 مل/د و طول موجة 272 نانومتر. كان زمن احتباس بنتوكسيفيلين نحو 14 دقيقة. جرى توثيق مصدوقية الطريقة فيما يتعلق بالخطية، الدقة، المضبوطية، و الانتقائية. أثبتت الخطية على مجال من 0.6 مكغ/مل الى 3.5 مكغ/مل و كان R2 0.994. كانت الطريقة مضبوطة (قيم استعادة 100.1%) و دقيقة (RSD > 2%). كان حد الكشف و حد الكم 0.2 مكغ/مل و 0.4 مكغ/مل على التوالي. طبقت الطريقة بنجاح لمقايسة بنتوكسيفيلين في المضغوطات مديدة التحرر المتوفرة في السوق السورية.
The present study describes a simple stability-indicating reversed-phase HPLC assay for pentoxifylline in its pharmaceutical dosage forms. Separation of the drug and the degradation products، under stress conditions was successfully achieved on a C18 column utilizing water: MeOH (60:40 v/v)، pumped at a flow rate of 1 ml min-1 with UV detection at 272 nm. The retention time of pentoxifylline was about 14 min. The method was satisfactorily validated with respect to linearity، precision، accuracy and selectivity. The response was linear in the range of 0.6-3.5 μg/ml with R2 0.994. The method was accurate (recovery 100.1%) and precise (RSD < 2%). Detection and quantification limit were 0.2 μg/ml and 0.4 μg/ml respectively. The suggested method was successfully applied for the analysis of pentoxifylline in extended release tablets available in Syrian market.
References used
Monteiro J. P.، Alves M. G.، Oliveira P. F.، Silva B. M.، Structure-Bioactivity Relationships of Methylxanthines: Trying to Make Sense of All the Promises and the Drawbacks. Molecules، Vol 21، 2016، ، 974-1006
McCarty M. F.، O'Keefe J. H.، DiNicolantonio J. J.، Pentoxifylline for vascular health: a brief review of the literature. Open Heart. Vol. 3، 2016، doi: 10.1136/openhrt-2015-000365
Bauza M.T.، Smith R. V.، Knutson D. E.، Witter F. R.، Gas chromatographic determination of pentoxifylline and its major metabolites in human breast milk. J. Chromatogr. Vol. 310، 1984، 61-69
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