Forty-five Bacillus isolates were obtained from different foods (spices,
grains, dried milk), the isolates were identified using traditional method from
Bergey's manual based on morphological tests (gram staining, spores staining),
physiological t
ests (optimum growth temperature, pH, NaCl concentration) and
biochemical tests (Voges-Proskauer, gelatin hydrolysis, esculin hydrolysis, β-
Galactosidase test, Lysine decarboxylase and Ornithine decarboxylase,
production of acid from different sugars). Bacterial suspensions were prepared
from the isolates and FTIR spectrums were obtained using ATR unit, which
consists of ZnSe prism, by scanning at the range of 4000-400 cm-1. Results
revealed that the isolates represented 5 species of Bacillus (B. pumilus, B.
subtilis, B. lentus, B. megaterium, B. cereus) and the potential of Fourier
transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR)
method as a reliable method for discrimination among Bacillus isolates, which
is accurate, rapid, easy to apply and cheap.
Eighty four bacterial strains, isolated from various local soils, vegetables
and fruits in decomposition were screened to detect pectinase using citrus
pectin as the sole carbon source according to solid plate assay.
Results showed that 13 strains
showed ability to produce pectinase
depolymerization in assay plate as evidenced by clear hydrolization halos
formed around the colonies. Four strains among them presented considerable
pectinolytic activity since haloes with more than 3 cm in diameter were formed.
Based on morphological and chemical characteristics, 3 strains were classified
as Bacillus subtilis and 1 strain as Bacillus cereus. The Bacillus subtilis (B30)
strain which had the biggest halo and produced high activity of the enzyme was
incubated with polygalactuoronic acid under different conditions of
temperature and pH levels. This strain showed high activity to produce
polygalauoronase at 52°C and pH 9.0. It was concluded that there is an ability
to produce locally alkaline polygalactuoronase which can be used for
manufacturing purposes, especially for treatment the west products of food
processing units to remove pectins.
A field experiment was carried out during 2003 and 2004 in Edleb
governorate to study the effect of phosphate solubilizing bacteria (Bacillus
megaterium) and cows manure on solubilization of phosphate rock, cotton
productivity, and phosphate uptak
e. The amounts of phosphate rock and
superphosphate were added by the same amount of P2O5.
An amount of nitrogen fertilizer as urea, was added to treatment 1, 2, 3, 4,
5, and 6 , whereas treatment 7, 8, 9, 10, 11, and 12 received amounts of organic
fertilizer having the same amount of N added to treatment 1-6 (MAAR
recommendation).
The research was carried out at Jeb Ramlah Research Station – AlGhab Research Center -
General Authority of Scientific Agricultural Research at 2017 growing season. The
objective was to investigate the effect of three organic and bio fertilizers (B
acillus, Humic
acid and Amino acids) and three fertilization methods (Soaking seeds, Vegetative spraying
and Plant irrigation) in some morphological and production traits of cotton cultivar 124
(Gossypium herutum L.) The experiment was conducted according to RCBD with three
replications. The first factor (fertilizer type) occupied the main plots, while the second
factor (fertilization method) occupied the split plots. Statistical analysis was carried out
using the Genestat.12 software and the least significant difference L.S.D. at 5% level was
calculated. Studied traits included (at the beginning of Blooming stage): Plant Height,
Plant wet weight, Plant dry weight, Leaf area and plant production of cotton. The results
showed that the average of treatment of amino acids was superiority significant on the
others in terms of plant height (70.70cm), Plant wet and dry weight (201.50, and 83.41g),
respectively, Leaf area (4240.15cm2/plant) and plant production of cotton (75.13g). For
fertilization method, Vegetative spraying average was superiority significant on the others
in terms of all studied traits.
