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Register a new userIsolation of local Bacillus spp. Strains and Testing its Efficiency Against Wax Worm Galleria mellonella L. Larvae in Laboratory
عزل سلالات محلية من بكتيريا .Bacillus spp و اختبار فاعليتها ضد يرقات فراشة الشمع الكبيرة .Galleria mellonella L مخبرياً
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المجلة السورية للبحوث الزراعية ورقة بحثية
Publication date
2018
fields
Plant Protecion
and research's language is
العربية
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أجريت هذه الدّراسة لتقدير فاعليّة سبع عزلات محليّة من جنس البكتريا Bacillus لمكافحة يرقات فراشة الشمع الكبيرة .Galleria mellonella L في مخابر الهيئة العامة للبحوث العلمية الزراعية عام 2012، جُمعت اليرقات المصابة من أقراص الشمع المخزنة، و عُزلت البكتريا من الجنس Bacillus من اليرقات الميتة، أو التي ظهر عليها أعراض مرضيّة، على شكل بقع بنية مسودة على جدار الجسم (الكيوتيكل). فُحصت البكتريا بعد تنميتها على الوسط الغذائي T3 و عُرفت اعتماداً على الاختبارات البيوكيميائية، و اختُبرت فاعليتها على مستعمرة نقية من يرقات فراشة الشمع ضمن ظروف المخبر. بيّنت نتائج الاختبارات البيوكيميائية أنّ العزلات تتبع للنوع Bacillus thuringiensis . كانت القدرة الإمراضية متباينة ما بين العزلات، فقد كانت العزلة Bt5 الأكثر كفاءة في قتل يرقات فراشة الشمع الكبيرة بفاعلية قدرها 72.41%، متفوقةً معنويّاً على جميع العزلات المختبرة عند مستوى احتمالية 0.01، و تفوقت العزلة Bt1 معنويّاً على الشاهد، و ظاهريّاً مع العزلات الأخرى و بفاعلية قدرها 27.59%، بينما كان الفارق ظاهرياً بين العزلات Bt2، Bt3، Bt4، Bt6 وBt7 و الشاهد عند مستوى الاحتمالية ذاته.
This study was carried out to determine the efficiency of local Bacillus isolates to control large wax moth larvae (Galleria mellonella L.) at GCSAR laboratory in 2012. Infected larvae were collected from stored wax combs, and the bacteria Bacillus genus were isolated from dead larvae, or that showing disease symptoms of black brown spots on larvae cuticle. Bacterium was grown on T3 medium and identified according to biochemical tests, and the efficacy of isolates was determined on pure colonies of larvae. The results of biochemical tests showed that the isolates belong to Bacillus thuringiensis. Isolates were different in pathogenicity. Bt5 isolate was the most efficient to kill the larvae of large wax moth (72.4 %), and significantly superior all other isolates (p ≤ 0.01). Bt1 isolate showed a significant difference with control but non-significant difference with Bt2, Bt3, Bt4, Bt6 and Bt7 isolates.
References used
Abbott, W.S. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol., 18: 265-267
Ararso, Z.; and G. Legesse (2016). Insecticidal action of honeybees propolis extract against larvae of lesser wax moth. Agriculture and Biology Journal of North. America. 7(6): 302-306
Bagdanov, S.; V. Kilchenmann; K. Seiler; H. Pfefferli; T. Frey; B. Roux; P. Wenk; and J. Noser (2004). Residues of paradichorobenzene in honey and bees wax. Journal of Apicultural Research. 43: 14-16
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The research has carried out at the Faculty of agriculture entomology lab.
and agriculture research center at Duma . Samples have been collected from
wax frames infected with Galleria mellonella (Lepidoptera: Pyralidae) From
Damascus, Damascus rural areas, Qunytera, Daraa and Tartus Governorats,
for three years (١٩٩٩ – ٢٠٠١).
Fifty eight isolates of lactic acid bacteria were obtained from different
local fermented foods. Isolates were identified using microscopic, cultural and
biochemical tests using API strips. Results showed that 44.83% of the isolates
were rod shape
d and 55.17% belonged to cocci bacteria.
Six different isolates of the fungus Beauveria bassiana were isolated from local
soils, which collected from two different ecosystems (Natural and Cultivated), at the
Syrian Coastal region, using the "Galleria Bait Method". The mortality caused by
these
fungal isolates against Galleria mellonella larvae was 100% after 3- 5 days post
treatment. The pathogenicity of these isolates was studied against 3rd instar larvae of
Spodoptera littoralis to detect the most virulent isolate. The mortality ranged from 75% to
100% for the all isolates at the 5th day post treatment. Spore suspension of the most
virulent isolate was used at concentration 106spore/ml by spraying it on the pupae of S.
littoralis. The percentage of adult emergence was 42% in the fungal treatment compared to
the control treatment.
Some species of the Syrian Flora were waned, other were extincted.
therefore, it was so nessesary to make a releve for restrict and classify the
survival species in order to put them under conserving & improvement genetic
program. The aim of this
research was to study the plant diversity in West
Homs; one of important basaltic region in Syria, and to concentrate on the wild
legumes and the Rhizobium strains which cohabit with. A plant survey was
carried out showed the existence of 365 species belong to 202 genera and 51
families, 15 species are endemic.12 species not observed previously in Syria but
are found in Lebanon were recorded,in addition another 3 species not listed at
all neither in Syria nor in Lebanon were mentioned. The geobotanical
attributes and the economical importance of the gathered species were
determined.as so as systematic keys of the most important families were
suggested...The root nodules of 10 species belong to 10 genera of wild legumes
were studied. Rhizobium strains were isolated and cultured in vitro. The
morphologic characters were studied microscopy. The pure strains were
reserved by refrigerator.
We aimed in this study to evaluate the effectiveness of Syrian propolis on some bacterial strains isolated from Al-Assad hospital in Latakia, to estimate its antimicrobial activity. Thus, we studied two propolis samples from two regions in the Syrian
coast, and prepared ethanolic extracts of propolis (EEP) with different concentrations (0.5%, 1%, 5%, 10%, and 20%). Then we tested these extracts on four strains of bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter baumannii), using pore method on Muller Hinton Agar. As we observed, the bacteria showed diverse susceptibility to the extracts and the most susceptible bacterium was Staphylococcus aureus which was sensitive to all concentrations and especially for EEP 20%. However, the least affected bacteria were E. coli and Pseudomonas, as the 0.5% and 1% EEP had no efficacy. On the other hand, there were little differences in antimicrobial activity between the two samples of propolis.
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