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Effect of treatment of the tomato seeds with strains of PGPR for inducing systemic resistance against cucumber mosaic virus in green house

تأثير معاملة بذور البندورة بسلالات من PGPR في تحفيز المقاومة الجهازية ضد فيروس موزاييك الخيار في الزراعة المحمية

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 Publication date 2016
  fields Plant Protecion
and research's language is العربية
 Created by Shamra Editor




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The study was conducted to evaluate the efficiency of Pseudomonas chlororaphis MA342, Serratia plymuthica HRO-C48K, Bacillus subtillis B2g, B. subtillis FZB27 to induce systemic resistance in Tomato plants against Cucumber mosaic virus (CMV) under greenhouse conditions. Tomato seeds submerged in suspension of Pseudomonas chlororaphis MA342, Serratia plymuthica HRO-C48, Bacillus subtillis B2g and B. subtillis FZB27 9×109cfu . plants emerged from these seed were inoculated with CMV after 10 days of planting. The time of beginning symptoms was recorded, Disease incidence, disease severity, area under disease progress curve AUDPC, progress of infection disease degrees during the study period were calculated using virtual symptoms . pyroxidas enzyme activity was determined, Some growth parameters such as Plant height, foliage fresh weight, root fresh weight , foliage dry weight, root dry weight were observed after 30 DAI. Results of this study showed that seed treatment with Pseudomonas chlororaphis MA342, Serratia .plymuthica HRO-C48, Bacillus subtillis B2g, B. subtillis FZB27 significantly decreased the diseases incidence on treated and inculated plants which ranged between 40% - 66.66% ,compared with un treated and inculated control 93.33% Without the presence of significant differences between the bacterial treatments. significantly decreased the diseases severity on treated plants which ranged between 45.53 %- 62.2% compared with inculated and un treated control 88.86% .Without the presence of significant differences between the bacterial treatments. Peroxidas activity on treated plants increased by Compared with untreated plants. Seed treatments with bacteria significantly increased the plant growth. The plant height , fresh and dry weight of foliage, fresh and dry weight of root on treated plants were significantly higher than those of inculated and un-treated plants according to LSD 5%.where bacteria reduced the rate of stunting and the rate of decline mild and dry weight of foliage and mild and dry weight of the root occurring due to infection with the Cucumber mosaic virus (CMV).


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Research summary
تتناول هذه الدراسة تأثير معاملة بذور البندورة بأربع سلالات من البكتيريا المحفزة لنمو النبات (PGPR) على تحفيز المقاومة الجهازية ضد فيروس موزاييك الخيار (CMV) في الزراعة المحمية. السلالات المستخدمة هي Pseudomonas chlororaphis MA342، Serratia plymuthica HRO-C48، Bacillus subtilis B2g، وBacillus subtilis FZB27. تم غمر بذور البندورة في معلق بكتيري لكل سلالة على حدة، ثم زرعت النباتات الناتجة وأعديت بفيروس CMV بعد 10 أيام من الزراعة. تم تقييم تأثير المعاملة من خلال قياس نسبة وشدة الإصابة، نشاط إنزيم البيروكسيداز، ومعايير النمو مثل ارتفاع النبات والوزن الطري والجاف للمجموع الخضري والجذري. أظهرت النتائج أن معاملة البذور بالمعلقات البكتيرية أدت إلى خفض معنوي في نسبة وشدة الإصابة وزيادة نشاط إنزيم البيروكسيداز في النباتات المعاملة مقارنة بالنباتات غير المعاملة. كما أدت المعاملة إلى تحسين معايير النمو بشكل عام، مما يشير إلى فعالية السلالات البكتيرية في تحفيز المقاومة الجهازية وتحسين نمو النباتات المصابة بفيروس CMV.
Critical review
دراسة نقدية: تعتبر هذه الدراسة خطوة مهمة في مجال البحث عن بدائل بيولوجية لمكافحة الأمراض الفيروسية في النباتات، وخاصة في ظل التحديات البيئية والصحية المرتبطة باستخدام المبيدات الكيميائية. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل توسيع نطاق الدراسة ليشمل مواسم زراعية مختلفة وظروف بيئية متنوعة لضمان تعميم النتائج. ثانياً، لم يتم التطرق بشكل كافٍ إلى الآليات البيوكيميائية التي تفسر كيفية تحفيز البكتيريا للمقاومة الجهازية، وهو ما يمكن أن يكون موضوعاً لدراسات مستقبلية. أخيراً، كان من الممكن تقديم تحليل اقتصادي لتكلفة وفوائد استخدام هذه البكتيريا مقارنة بالمبيدات التقليدية، مما يعزز من جدوى التطبيق العملي لهذه النتائج.
Questions related to the research
  1. ما هي السلالات البكتيرية المستخدمة في الدراسة؟

    السلالات البكتيرية المستخدمة هي Pseudomonas chlororaphis MA342، Serratia plymuthica HRO-C48، Bacillus subtilis B2g، وBacillus subtilis FZB27.

  2. ما هو الهدف الرئيسي من الدراسة؟

    الهدف الرئيسي هو تقييم تأثير معاملة بذور البندورة بسلالات من البكتيريا المحفزة لنمو النبات على تحفيز المقاومة الجهازية ضد فيروس موزاييك الخيار في الزراعة المحمية.

  3. ما هي المعايير التي تم استخدامها لتقييم فعالية المعاملة البكتيرية؟

    تم تقييم الفعالية من خلال قياس نسبة وشدة الإصابة، نشاط إنزيم البيروكسيداز، ومعايير النمو مثل ارتفاع النبات والوزن الطري والجاف للمجموع الخضري والجذري.

  4. ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟

    النتائج الرئيسية هي أن معاملة بذور البندورة بالمعلقات البكتيرية أدت إلى خفض معنوي في نسبة وشدة الإصابة وزيادة نشاط إنزيم البيروكسيداز، بالإضافة إلى تحسين معايير النمو بشكل عام.


References used
ALTUNKAYA, A.; GOKMEN, V. Purification and Characterization of Polyphenol Oxidase, Peroxidase and Lipoxygenase from Freshly Cut Lettuce (L. sativa). Biotechnol. 49, 2011, 249–256
BEHERA, S.; GHANTY, S.; AHMAD, F.; SANTRA, S.; BANERJEE, S. UVVisible Spectrophotometric method development and validation of assay of Paracetamol tablet formulation, J. Anal Bioanal Techniques. 3, 2012, 1-6
CHEN, X.; LINE, R.F.Gene action in wheat cultivars for durable, hightemperature adult plant resistance and interaction with race – specific, seedling resistance to pucciniastrii formis. Phytopathology, 85, 1995, 567-572
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