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Amelogenins are the major enamel proteins that play a major role in the biomineralization and structural organization of enamel , Aberrations of enamrl-related proteins are thought to be involved in oncogenesis of odontogenic epithelium. The expre ssion of amelogenin is possibly an indicator of differentiation of epithelial cells in the odontogenic lesions.
:Bone morphogenetic proteins (BMPs) belong to the family of transforming growth factors-β (TGF-β) and play an essential role in the cellular proliferation and differentiation. Trails that used immunohistochemistry have shown that Ki67 is existed i n all normal cells which is in proliferating phase and absent in differentiated cells in resting phase. Aim : to investigate the effects of rhBMP-2 on the proliferative activity of rabbits bone tissue using Ki67 staining.
the leishmaniasis is a serious health problem in Syria, due to the wide spreading of Leishmania Tropica parasite , the hardness of controlling the reservoirs of parasites, and there is no specific ,safe active therapy. A Heat shock protein 20 HSP2 0 belongs to the small HSP (sHSP) family.
Kidney Injury Molecule-1 (KIM-1) is a type 1 transmembrane glycoprotein. KIM-1 is highly up regulated in proximal tubular cells following kidney injury. It consists of an extracellular portion, a novel 6-cysteine immunoglobulin-like domain, 2 N-gl ycosylation sites, also contains one transmembrane domain and a short cytoplasmic domain with a conservative tyrosine phosphorylation site.
The Ultrafiltration (UF) technology was conducted on samples of akawe cheese whey to obtain the non-protein and fat whey (permeate) that rich in lactose and was concentrated by evaporation and carefully prepared after fortification by nutrients, (0.2) gr/100ml (NH4)2Hpo4 and (0.2) gr/100ml MgSo4, and (0.3) gr/100ml (NH4)2SO4, and prepared as a fermentation medium for yeasts capable of metabolizing sugar, and after that (15) samples were chosen and chemically analyzed along with the whey. Pure isolated Kluyveromyces lactis (42-K) yeast, recognized by ß- galagtosidase enzyme and its important application in food, medicine and pharmacy, was proliferating on the fermentation medium. The optimal conditions were determined to obtain single cell protein (SCP). The results referred that typical conditions of yeast growth were (6.5)% lactose concentration, pH (5), temperature (30°c), Fermentation time (24) hour, and the optimal speed of incubator shaker (350) rpm and the typical average of aerator (8%). The optimal values were applied in the bio fermentor (electrolab) to obtain biomass which was heated at (75-90 c°) for the extraction of the Single Cell Protein (SCP). The chemical analysis was conducted on (15) samples of single cell protein (SCP), and the results were in accordance with all the Syrian special specification, and (FOA) specification. The microbial estimated results met (FOA) specification, and the product was free from toxicity elements and cancered factors, which proved that the product was with high quality of proteins. The single cell protein (SCP) was added to Labneh, that manufactured by direct method, and to the proceed speared cheese between (1-10%), alternately with dried skimmed milk. The chemical and microbial analysis of Labneh met all the Syrian special specification and international codex. The result of sensory evaluation referred that percentage of single cell protein (SCP) must not exceed (4%) in the Labneh. The chemical and microbial analysis of proceed speared cheese met all the Syrian special specification and international codex. The result of sensory evaluation referred that the percentage of single cell protein (SCP) must not exceed (3%) in the proceed speared cheese. The results of a simplified feasibility study on the Labneh supported by single cell protein (SCP) (4%), and proceed speared cheese supported by single cell protein (SCP) (3%), referred to good gaining both of the products.
The lack of information about the cultivation of coriander plant in Syria prompted us to study the effect of four plant densities (33.33،10، 13.33 and 20 plant/m2)، and four levels of phosphate fertilization (0107.2،160.8، and 214.4 of Superphospha te 46%) on some productive and quality traits of the coriander plant. This study was carried out in Tartous province during the growing season 2014، using Randomized Complete Block design with a Split Plot arrangement of treatments. The Results revealed that there were significant differences between the treatments for the whole traits، in addition to a significant interaction between density and phosphate fertilization. Although the densities (10، 13.33 and 20 plant/m2) varied in their effect، they had superiority upon the control(33،33)plant/m2، and the density 10 plant/m2 had more important effect in terms of increasing each of: branches number/plant، seed weight/plant، seeds number/inflorescence، protein content in plants and seeds and beta carotene and chlorophyll. However، the level 160.8k.g/h of P2O5 increased the number of inflorescences/plant، number of fruits/plant and seed weight/ inflorescence.
This research was conducted in the countryside of Tartous, to aim for the analysis of moisture, protein, TSS and pH of royal jelly (RJ) harvested after 24 to 48 hours of grafting, compared with that traditionally harvested after 72 hours of grafting. As well as to analysis of these compounds in (RJ) within cells to brood of workers, compared with the (RJ) product in cups. 50 samples were analyzed in wet weight and dry weight for (RJ), which was harvested in this research.
The aim of this work was determining the role of Protein kinase C (PKC) signaling pathway in apoptosis of Leishmania tropica promastigotes, through studying effects of PKC specific inhibitor Staurosporine (STS) on the parasite. Our results showed that treated L. tropica with Staurosporine was enough to inhibit PKC pathway completely, while viability test showed that the concentration of STS to inhibit cell growth by 50% (IC50 = 2.146μM).
Background & Objective: Spray drying techniques are used to produce inhaled powders of enzymes and proteins without denaturation. The surface free energy data of these powders is used to reduce the time of formulation development. During storage, the surface free energy of the inhaled protein powders could change and so their performance will be affected. Many factors affect the limit of this change. Storage temperature is the critical factor. Material& methods: A spray drying method was optimized to prepare inhaled spray dried lysozyme powders. Inverse gas chromatography was utilized to measure the surface free energy of the prepared powders pre and post-storage to detect the effect of storage temperature on the surface free energy. Differential Scanning Calorimetry and biological activity tests were used to evaluate the integrity of lysozyme conformation after storage at different storage temperature. Results: Increasing the storage temperature decreased both dispersive and specific surface components of the spray-dried lysozyme powders significantly (ANOVA: P < 0.001) up to 12% and up to 31 % after storage for one year at 40 °C, respectively. The overall decrease in the polar components resulted in a reduction of the surface basicity. These decreases in the surface energetics would be due to the aggregation of denatured lysozyme molecules at the powder surface via hydrophobic regions rich with electrons. The same results were found after storage for one year at room temperature, but the extents of changes were smaller. Conclusion: It is advisable to store protein powders at 5°C to inhibit the changes in their surface free energy which is the crucial factor affecting their performance.
A random sample of the kinds of bread consumed in the coastal region during the years of research was taken, the percentage of fiber and protein was calculated, and the effect of the mixture approved on an annual basis in mills on purveyance flour content of fiber and protein was studied. The study showed the important role of the mixture, where the percentage of fiber increased from (1.06%) in 2009 to (1.61%) in 2010, and the percentage of protein increased also from (11.36%) in 2010 to (13.90%) in 2012. The results show that there are some, but not all, governmental mills which add soft bran fiber and protein-rich flour, taking into consideration the impact of technological processes applied throughout the stages of manufacturing bread, and in particular the stages of fermentation and broil.
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