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Register a new userEffects of rBMP-2 on the proliferative activity of the bone tissues in rabbits
تأثير بروتين 2-rhBMP على النشاط الانقسامي للنسيج العظمي عند الأرانب
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Aِl-Baath University ورقة بحثية
Publication date
2016
fields
Dentistry
and research's language is
العربية
Authors
شريف بركات( باحث )
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Shamra Editor
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تنتمي بروتينات التخلق العظمي BMPs إلى عائلة عوامل النمو المحولة TGF-β و تلعب دورا محورياً في عمليتي التمايز و التكاثر في الخلايا. أظهرت التجارب التي اعتمدت على التلوين المناعي تواجد Ki67 في جميع الخلايا الطبيعية في طور التكاثر و غيابه في الخلايا المتمايزة الواقعة في طور الراحة. هدف البحث : تحري تأثير بروتين 2-rhBMP على النشاط الانقسامي لخلايا النسيج العظمي للأرانب باستخدام ملون Ki67.
:Bone morphogenetic proteins (BMPs) belong to the family of transforming growth factors-β (TGF-β) and play an essential role in the cellular proliferation and differentiation. Trails that used immunohistochemistry have shown that Ki67 is existed in all normal cells which is in proliferating phase and absent in differentiated cells in resting phase. Aim : to investigate the effects of rhBMP-2 on the proliferative activity of rabbits bone tissue using Ki67 staining.
References used
Kempen، D.H.، et al.، Growth factor interactions in bone regeneration. Tissue Eng Part B Rev، 2010. 16(6): p. 551-66
Moy، P.K.، S. Lundgren، and R.E. Holmes، Maxillary sinus augmentation: histomorphometric analysis of graft materials for maxillary sinus floor augmentation. J Oral Maxillofac Surg، 1993. 51(8): p. 857-62
Langer، R. and J.P. Vacanti، Tissue engineering. Science، 1993. 260(5110): p. 920-6
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Epithelial to mesenchymal transition (EMT) process represents an
essential stage for tissue healing، it is also considered as a preface
for tumour proliferation and invasion. The current study aimed to
investigate the effects of rhBMP-2 protein on
two essential
biomarkers for this process : β-catenin and E-cadherin.
This study was conducted to investigate the relationship between estrogen
depletion ,calcium-deficiency,and alveolar bone loss.
Alveolar bone loss was evaluated by radiographic and visual inspection of
rabbits with experimental lack of estrogen an
d calcium.
Twenty female local rabbits (4 months old) were divided into four groups
:group a -ovariectomized and given a standard solid diet.
Group b-ovariectomized and given a calcium – deficient diet
Group c-sham-ovariectomized and given a standard solid diet.
And group d-sham-ovariectomized and given a calcium-deficient diet
after 8 weeks,the rabbits were sacrificed .
The maxillae, mandibles, femurs, and tibias were removed carfully and
fixed in 10% neutral buffered formalin. The bone mineral density of each
bone and the alveolar bone loss were measured.the bone meniral densities of
the maxillae,mandibles,femurs and tabias in group (c) were significantly
higher than those in groups (b)and (d),but not higher than those in group
a.however there were no significant differences between any of the groups
with regard to alveolar bone loss from the cemento-enamel junction to the
molar bone crest.
Aim of the Study:
Investigating the effect of adding rh-BMP2 on the expression of
vascular endothelial growth factor VEGF.
Recombinant human bone morphogenetic protein-2 (rhBMP-2 ) is
effectively to regenerate defects of the maxillary and mandible
bones. Such defects are sometimes caused by resection of oral
squamous cell carcinoma (OSCC) yet the biologic effects of
rhBMP-2 on this malignancy and its histological relations and the
ability of invasion and metastasis are not fully clear.
Aim of the study : this study aimed to investigate the effects of
rhBMP2 on E-cadherin expression , which plays a pivotal role in the
cellular adhesion through the adherent junctions.
Background and Objective : Bone morphogenic protine-2 (BMP-2) plays an essential
role in mesenchymal cells differentiation into osteoblasts، through many intracellular
pathways which crosses with tumoral pathways . Oral squamous cell carcinoma is a
n
invasive epithelial neoplasm، counts more than 90% of head and neck malignancies. It is
classified into three degrees according to the epithelial cells differentiation. The study
aimed to investigate the changes in BMP-2 expression according to the histological
degrees of the tumor.
Materials and methods : In the present study، BMP-2 expression was compared
immunohistochemically among oral squamous cell carcinoma in 27 cases (19 male+8
female) ranging between (22-74) years، mean age of (48) years. Patients have been divided
into 3 groups (each contain 9 case) according to the histological grade.
Results : By analyzing the statistical results, No significant correlation between
BMP-2 expression and the histological grad was observed. The severity of expression was
not affected by the tumour degree. Changes in membranous localization and intense
cytoplasmic staining were also not observed.
Conclusion : according to this study BMP-2 does not represent a prognostic marker
for the histological grade of oral squamous cell carcinoma.
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