Aim: to assess the expression ofcathepsin B(ctsB; an important protease) in human
breast cancer and correlate that with the tumor grade.
Materials and Methods:Serial sections were obtained from 23 formalin-fixed paraffin
embedded breast cancer tis
sue specimens which were collected from Alasad University
Hospital and a pathological laboratory in Lattakia - Syria. Tissues were stained for
cathepsin B using immunohistochemistry. Tonsil tissues were also included as a positive
control.
Results:Positive cytoplasmic staining of CtsB was observed in both follicular
macrophages and endothelial cells in tonsil sections. In breast cancer tissues, tumor cells,
endothelial cells and stroma stained positive for CtsB. The presence of CtsB in blood
endothelium significantly correlated with the tumor grade (p=0.049).
Conclusion: These results supported the important role of cathepsin B in tumor
progression, particularly in angiogenesis. Future studies should investigate the regulating
mechanisms of cathepsin activities within the tumor environment, and that can be
considered as new tagets for cancer treatment.
In the present study, we measured DNMT3A levels, the
methylation enzyme, in blood samples from Syrian AML patients
and normal individuals using real-time quantitative polymerase
chain reaction, in order to determine the roles of DNMT3A in the
development of leukemia.
أجري هذا البحث بهدف معرفة تأثير إضافة مضادات الأكسدة الصناعية في الخلطات العلفية في الكفائة الإنتاجية لدجاج اللحم.
The study included the selection of the best methods to extract the enzyme
among nine methods. The protein content was concentrated and precipitated
by cold acetone among other five methods of concentration (partial
purification). The purification
stages were achieved by using ion exchange
column chromatography (DEAE – Sephadex A 50 column). Followed by gel
filtration chromatography using sephacryl S-300. The active parts were
lyophlizated (free drying) to obtain Lipoxygenase with 43.18% yield and 8.16
folds of purification and specific activity of 1162.9 unit / mg.
The purity of enzyme was confirmed by poly acryl amide gel
electrophoresis under non denaturing conditions, with the appearance a single
band .
نفذ البحث على 925 صوص فروج من الهجين المسمى (لوهمان)، وزعت بالتساوي إلى خمس
مجموعات بمعدل 185 طيرًا في كل مجموعة، سمنت طيور كل مجموعة في غرفة مستقلة من النموذج
المفتوح، و كانت جميع ظروف الإيواء و الرعاية واحدة لجميع المجموعات. كما غذيت طيور جميع ال
مجموعات خلال المرحلة الأولى (1-14 يومًا من العمر) على خلطة نباتية خالية من الشعير و الأنزيمات، أما خلال المرحلتين الثانية ( 15 - 35 يومًا من العمر) و الثالثة ( 36 - 49 يومًا من العمر) فقد غذيت
مجموعات الطيور المختلفة على خلطتين نباتيتين بعد إدخال الشعير المحلي مع الأنزيمات إلى كل منها
بنسبة 0% للمجموعة الأولى (الشاهد)، 20 % للمجموعة الثانية، 30 % للمجموعة الثالثة، 40%
للمجموعة الرابعة، 50 % للمجموعة الخامسة.