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Register a new userMeasuring Levels of DNMT3A Gene Expression Responsible For DNA Methylation in a Sample of Syrian Acute Myeloid Leukemia (AML) Patients
مقايسة مستويات التعبير عن الجين الناقلة للميثيل DNMT3A لدى عيّنة من مرضى الابيضاض النّقوي الحاد AML السوريين
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Aِl-Baath University ورقة بحثية
Publication date
2015
fields
Medicine
and research's language is
العربية
Created by
Shamra Editor
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توجهت دراستنا نحو مقايسة مستويات التعبير عن الجين الناقلة للميثيل DNMT3A عند جمهرة من مرضى ابيضاض دم نقوي حاد سوريين و مقارنتها مع مستوياتها لدى الأصحّاء من جهة، و ربطها بأعمار المرضى و نمط الابيضاض من جهة ثانية. في محاولة لتوضيح دورها في آليّات نشوء الابيضاض و تطوّره.
In the present study, we measured DNMT3A levels, the methylation enzyme, in blood samples from Syrian AML patients and normal individuals using real-time quantitative polymerase chain reaction, in order to determine the roles of DNMT3A in the development of leukemia.
References used
2000- Overview of acute myeloid leukemia. Am J Manag Care, Vol 6(18 Suppl), S971-2
HASSERJIAN R.P, 2013-Acute myeloid leukemia: advances in diagnosis and classification, Int J Lab Hematol, vol 35(3), 358-66
GOLDBERG AD,ALLIS CD,and BERNSTEIN E, 2007 Epigenetics: a landscape takes shape, Cell, vol 128(4), 635- 8
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Gene regulation by microRNAs (miRNAs) is one of the most important regulatory
networks which control normal hematopoiesis. Disturbances in miRNAs levels lead to
proliferation diseases including leukemogenesis. MicroRNAs is a major topic of many
ca
ncer researches performed to discover noninvasive biomarkers used for diagnosis,
prognosis, and optimization of clinical decision.
This study, the first to be performed in Syria, aimed at monitoring miR-155 levels
compared to a normalizer gene RNU6-2 by quantitative reverse transcriptase -PCR (qRTPCR)
in a sample of newly diagnosed untreated AML patients at several hospitals in
Damascus in comparison with healthy controls. Changes in miR-155 gene expression
levels were calculated in patients and controls using the 2-ΔCt method.
The most important finding was the association of low and very high miR-155 levels
with poor prognosis reflected in failure to accomplish complete remission and high
mortality. In addition, high miR-155 levels were associated with M4 AML subtype,
although with large variance among patients. We hope our preliminary study pave the road
for many future research studies related to the applicability of microRNAs in supporting
diagnosis, predicting prognosis, and enhancing the personalized therapies which deal with
patients as individual cases.
Hepatocellular carcinoma (HCC) is a common malignancy and the
leading cause of death worldwide, due to late detection and high
recurrence rates. Osteopontin (OPN) has various functions,
including prevention of apoptosis and modulation of angiogene
sis
which lead to tumor formation and progression, although the exact
mechanisms for the development of cancer are still unknown.
Serglycin is an intracellular proteoglycan in hematopoietic cells. It
has been studied in normal and tumor hematopoietic cell, so it was
suggested to have an important role in immunity and cancers as leukemia.
The challenge to make a diagnosis for
acute leukemia and to
differentiate between myeloid or lymphoblastic leukemia, is the reason
we go to assay concentration of serglyc in normal people and
patient with acute myeloid leukemia and acute lymphoblastic leukemia
from children and adult at hematology department in the
main hospitals in Damascus ( Pediatric’shopital, Almouasat and Al
asaad hospital) before beginning any kind of treatment and studying
the relation with white blood cells count. We found clear difference
between the concentration in patient with acute leukemia and normal
people. There was a high level of this marker in paint with
AML in contrast with ALL patients. We found a relation between
concentration of serglycin and the count of white blood cells.
In contrast to adaptive immunity and to certain components of innate immunity, only little is known on the influence of the complement system on cancer suppression and promotion . The lectin pathway of complement activation is an important part of in
nate immunity. It is mainly involved in opsonisation and lysis of pathogens and in recruitment of inflammatory cells.
Our study aimed to assess the relationship between MASP-2 serum levels before and after chemotherapy in Syrian children with acute lymphoblastic leukemia, aiming to find a relation, which serve in early forecast of patients response to chemotherapy, where the study has been done at Children University Hospital in damascus between 2013-2014. This study included 37 children diagnosed with acute lymphoblastic leukemia (ALL). The levels of Mannose-Binding Lectin-associated Serine Protease-2 (MASP-2) were determined by Enzyme Linked Immuno Sorbent Assay (ELISA).
The result was that serum levels of MASP-2 was strongly reduced after chemotherapy in children with acute lymphoblastic leukemia, and a strong correlation between serum marker levels before and after chemotherapy was found.
Aims of study: 1-To determine the prevalence and 2- the pattern of hypodontia in
the permanent dentition in a sample of Syrian orthodontic patients who sought orthodontic
treatment.
Materials and Methods: The orthodontic records of 623 patients ag
ed 12 to 34, who
sought orthodontic treatment in the Department of Orthodontics, Dental faculty/Damascus
University, during the last three years (2011-2014), were investigated to establish the
prevalence of patients with one or more permanent missing teeth.
Results: 1-The prevalence of hypodontia was 7.10% of the studied sample (7.49% in
females, and 6.35% in males). Males were less affected than females with a ratio of 1:1.18
with no significant differences between genders (P = 0.063).
2-The most common missing tooth was maxillary lateral incisor (56.52%), the least
common missing tooth was maxillary first molar (0.87%). Missing in the maxillary jaw
was significantly more frequent than the mandible (P=0.041( with significantly more
anterior teeth missing than posterior teeth (P=0.009).
Conclusions: The prevalence of hypodontia showed in this study was within the
range published in the literature. The patterns are similar to most reported patterns in the
literature.
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