A new analytical method has proposed for determination of
five synthetic colorants used in the wine industry (i.e., Tartrazine,
Brilliant Black, Sunset yellow, Brilliant Blue and Erythrosine) by
reversed-phase high-performance liquid chromatograph
y equipped
with Diode Array detector.
Experimental conditions, including spectral properties, limits
of detection and recoveries for these synthetic colorants in detail
were studied and assigned according to the statistical methods
adopted.
The possibility to develop a High-Performance Liquid
Chromatographic method was studied for simultaneous
determination of: Cu(II), Ni(II), Co(II), Hg(II) by using DTC as
reagent. The diethyldithiocorbamate Reagent was studied by
spectrophotometric Method, Unique Ionisation Constant was
determined and its value.
The purpose of this article is how to use HPLC technique for
quantitive analysis of cholesterol in some foods. The best conditions
are studied for this analysis including composition of mobile phase,
temperature, flow rate, the wavelength of detec
tor UV-VIS, and
acidity of mobile phase. The column used for cholesterol
determination by this method was: C18(5μm,250x4.6mm) with
injection volume of 20μL. Methanol 100% was used as mobile
phase.
spectrophotometric and chromatographic methods was used for
determination of five water-soluble vitamins, including : Thiamine
HCl (B1), Ascorbic acid (VC), Niacinamide (PP), Cyanocobalamin
(B12), Riboflavin (B2) in this research.
The conditions
of chromatographic separation were reached to the
vitamins with good resolution.
A simple and accurate method was developed for the analysis of
carbinoxamine maleate, dextromethorphan hydrobromide and
pseudoephedrine hydrochloride content in pure form and pharmaceutical
preparations using HPLC. Analysis was conducted on a sili
ca column (6 μm)
with mobile phase consisting of ethanol – ammonium acetate (0.05 M) in rate
[85:15] respectively, and at detection wavelength of 276 nmand flow rate 1ml/ min.
Results were linear (correlation coefficient R > 0.9996) in the range of the
studied concentrations for the active materials. The relative standard
deviations (n=6) of intra and interday assay were 0.931%, 1.527% for
carbinoxamine maleate and 0.717%, 1.058 % for dextromethorphan
hydrobromide and 0.309%, 0.891% for pseudoephedrine hydrochloride,
respectively. This method, proved to be easy, precise and economical, is useful
for quality control of pharmaceutical drugs industrial samples.
The purpose of this article is how to use HPLC technique for analysis of
some vitamins. The studied vitamins are niacinamide (vit.PP), pyridoxine
hydrochloride (vit.B6), riboflavin (vit.B2) and thiamine hydrochloride (vit. B1),
which can be found
in some Syrian pharmaceutical preparations.
