One of the most fascinating aspects of enzymes is the way these
biological catalysts have been designed in order to be able to carry
out highly selective reactions under mild conditions. Controlled
modification and artificial tuning of Nature’s legacy, using
molecular biology technices together with new chemical methods
in the field o f bioorganic syntheses, led chemists to investigate
access to modified proteins, going as far as designing artificial
enzymes.
spectrophotometric and chromatographic methods was used for
determination of five water-soluble vitamins, including : Thiamine
HCl (B1), Ascorbic acid (VC), Niacinamide (PP), Cyanocobalamin
(B12), Riboflavin (B2) in this research.
The conditions
of chromatographic separation were reached to the
vitamins with good resolution.
