spectrophotometric and chromatographic methods was used for
determination of five water-soluble vitamins, including : Thiamine
HCl (B1), Ascorbic acid (VC), Niacinamide (PP), Cyanocobalamin
(B12), Riboflavin (B2) in this research.
The conditions
of chromatographic separation were reached to the
vitamins with good resolution.
Vitamin C concentration was studied in samples of infant formula from 5 companies
(referenced A-E) using the 2,6-Dichlorophenolindophenol titration (AOAC, 2006) method.
Results revealed that ascorbic acid content in formulas D and C was the closest
to its
recommended daily intake (RDI) and to the concentrations which are mentioned on the
label of the samples. Storage conditions at room temperature or in the refrigerator did not
affect ascorbic acid levels. In comparison to vitamin C concentrations in milk dissolved in
tap water, its levels decreased when water was warm (40°C) or hot (100°C), and its
concentrations further declined in dissolved form kept for two hours after preparation.
Sour orange (Citrus aurantium L.) seedlings were sprayed three times a
season with four levels of urea (45 % N) (2.5, 5.0, 7.5 and 10.0 g/L) and two
levels of ascorbic acid (150 and 300 g/L), each alone or in combinations.
Meanwhile the seedlings
of control treatment sprayed with distilled water
during 2010 growing season, twenty days intervals between each spray and
another. Results obtained at the middle of October of the same season,
indicated that the foliar spray with urea at the concentrations of 5.0, 7.5 and
10.0 g/L significantly increased leaves N and chlorophyll concentrations, leaves
number, seedling leaves area, seedlings height, main stem diameter and leaves
fresh and dry weight, especially at the foliar spray with 10.0 g/L. The means of
these parameters were 3.19%, 66.80 SPAD, 146.66 leaves/seedling, 3700.00
Cm², 83.66 Cm, 4.50 mm, 4.70 g, 1.53 g, respectively. Meanwhile ascorbic acid
spray at two concentrations unsignificantly affected all parameters, except
leaves chlorophyll content, leaves number and seedling leaves area. Most
treatments of combinations spray with urea and ascorbic acid significantly
affected all studied parameters, especially the treatment of foliar spray of urea
and ascorbic acid at a concentration of 10.0 g/L and 300 mg/ L, which gave the
highest means of leaves N concentration, leaves number, seedling leaves area
and leaves fresh and dry weights, and it unsignificantly differs from the
treatments which gave the highest means of seedlings height, main stem
diameter and percentage of leaves dry matter weight .
Two experiments were conducted to evaluate the effect of ascorbic acid
(AA) during egg incubation on development of broiler chickens of a
commercial stock. In Experiment ١, eggs with living embryos were injected at
١٥ d of incubation with ٠٫١ ml o
f saline solution containing either ٣ or ١٢ mg of
AA per egg and uninjected control. Body weights of each treatment were
determined weekly from hatch to ٦ wk of age. In Experiment ٢, the treatments
at ١٥ d of incubation were as follows: ١) eggs injected with ٣ mg of AA and
then cooled at ٢٢° C for ٢٤ h; ٢) eggs dipped in ٣٪ solution of AA for ٣ min
and then cooled at ٢٢° C for ٢٤ h; and ٣) control. Embryo weight at ١٩ d of
incubation, hatchability and body weights of the hatched chicks were
determined.
