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Register a new userAnalysis of some Vitamins in some Food Products by Using High Performance Liquid Chromatographic Method HPLC
تحليل بعض الفيتامينات في بعض العينات الغذائية باستخدام طريقة الكروماتوغرافيا السائلة عالية الأداء HPLC
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تمّ في هذا البحث تحديد خمسة فيتامينات منحلة في الماء باستخدام طرائق طيفية و كروماتوغرافية و هي: ثيامين هيدروكلورايد (B1), حمض الإسكوربيك (V.C), النياسيناميد (PP)، سيانوكوبلامين (B12), ريبوفلافين (B2). و تمّ التوصل إلى شروط فصل. كروماتوغرافي بتفريق جيد لها، حُدّد المجال الخطي بين مساحة القمة الكروماتوغرافية و تركيز الفيتامين.
spectrophotometric and chromatographic methods was used for determination of five water-soluble vitamins, including : Thiamine HCl (B1), Ascorbic acid (VC), Niacinamide (PP), Cyanocobalamin (B12), Riboflavin (B2) in this research. The conditions of chromatographic separation were reached to the vitamins with good resolution.
References used
Combs, Jr., 2008- The vitamins: fundamental aspects in nutrition and health. Elsevier Academic Press, 3rd ed. USA, 603p
BALL, G.F.M., 2006- Vitamins in foods: analysis, bioavailability, and stability. Taylor & Francis, USA, 814p
FENNEMA, O.R., 1996- Food Chemistry. Marcel Dekker, Inc., 3rd ed. USA, 1262p
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The purpose of this article is how to use HPLC technique for analysis of
some vitamins. The studied vitamins are niacinamide (vit.PP), pyridoxine
hydrochloride (vit.B6), riboflavin (vit.B2) and thiamine hydrochloride (vit. B1),
which can be found
in some Syrian pharmaceutical preparations.
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