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In Vitro Embryo Production in Syria Goats

إنتاج الأجنة خارج الرحم في المعز السوري

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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References used
الخوري , فارس 1996 موسوعة عروق المعز في الدول العربية المركز العربي لدراسة المناطق الجافة والاراضي القاحلة ( اكساد) دمشق
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This study was conducted at the biotechnology laboratory of AnimalWealth Research Administration, General Commission for Agricultural Research in order to compare the effect of different media on In vitro maturation (oocytes matured at 39 C° under 5% CO2 for 24 h), fertilization، and culture (7 days postfertilization) in humidified atmosphere 95% on bovine embryo production.
The wide geographical spread and the exponential growth of the numbers of goats around the world clearly demonstrate the ability of these ruminants to adapt to harsh climates and grazing land. The aim of this study was to assess the genetic divers ity of 42 samples of Syrian goats from many domestication stations including Jabali, Shami and hybrid (hybridization between both Shami and Jabali). The study was done by DNA extraction of these samples, application of SSR technology using 7 microstellite markers. The alleles number of markers were 29 alleles, at a rate of 4.1 allele for each genetic locus. The number of alleles of each locus ranged from 3 alleles in genetically markers (BMS1714, INRAD07, SRCRSP09) to 8 alleles at the genetic marker SRCRSP01. The rate of allele-frequency across all studied genetic sites ranged from 0.071429 with the molecular size of 210 bp for the McM527 genetic site to 0.97619 with a molecular size of 55 bp for the SRCRSP09 genetic site.
This research was carried out in four different regions on the Syrian coast on four herds of Shami goats different in numbers. These were grown in different ways depending on farmers' concepts and needs, and the geographic location of each region. T he number of goats that were studied was (44). The daily average of milk production per goat was calculated, in addition to the monthly average and overall average for every herd during the lactation season which was divided into two phases (Breastfeeding, Lactation) .In the first phase it was (2493,9 – 2238,6 – 1776,6 – 81,6) L ordered by herd and method of breeding (4 – 2 – 3 – 1) and same was in the second phase. The average production rate was (54,27)% in first method of first herd and in other methods was lower than (50)% and increased in other herds (49,39 – 45,36 – 44,16)% respectively (2 –4 – 3 – 1). It was noticed that in the second phase of Lactation the herd of first method retracted to lower than (50)%and rose in other herds (55,83 – 54,63 – 50,60 – 45,72 )% Respectively (1 –2 – 4 – 3). Statistical analysis demonstrated the lack of significant differences in second and forth herd in spite of the different methods used in the other herds, and in second phase statistical analysis was in four herds in accordance with different methods of breeding.
This Research was conducted in the laboratory of tissue culture affiliated to the National Commission of Biotechnology (NCBT) during the period between 2011 -2012 for induction of callus from mature embryos for three local Genotypes of grain Sorgh um, regeneration of the plant from callus, rooting plantlets and acclimatization in order to get a plant capable to grow in greenhouse. The best concentration for sterilizing the plant and entering it in use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4- dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of callus induction and embryogenic callus.
The main objective of this research is to estimate the potential electrical power that can be extracted from closed and out-of-service oil wells situated in the north-eastern part of Syria. The research shows that using Organic Rankine Cycle (ORC) wi th Isobutane as working fluid can produce net power output under certain conditions. The geothermal cycle used brine solution to absorb the ground heat.

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