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Register a new userMapping and Identification of a Powdery Mildew Resistance Gene in a Barley (Hordeum vulgare L.) cross
تحديد مورث المقاومة لمرض البياض الدقيقي و المؤشرات الدناوية المرتبطة به في هجين من الشعير (Hordeum vulgare L.)
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Damascus University ورقة بحثية
Publication date
2002
fields
Plant Protecion
and research's language is
العربية
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تم اختبار الأبوين ٢٢٩١ Tadmor, WI من الشعير، الواسعي الانتشار في زراعتهما في مناطق البحر الأبيض المتوسط، مقارنة مع مجموعة السلالات المفرقة الأوربية للبياض الدقيقي، ضد عزلات فطرية. استخدم لهذا الإختبار ست عزلات معزولة مخبريًا بطريقة البوغة الواحدة، جمعت أربع منها من على الأصناف السورية و عزلتان من على الأصناف الدانمركية، حيث استخدمت هذه العزلات في غربلة مستويات المقاومة المختلفة.
WI٢٢٩١ and Tadmor, two barley lines widely grown in the Mediterranean region were tested for powdery mildew (Erysiphe graminis f. sp. hordei) resistance together with the European differential tester set against six powdery mildew isolates. The six cultures of powdery mildew developed from single colonies were comprised of four powdery mildew isolates originating from Syrian barley lines and two cultures originating from Danish barley lines.
References used
Aslam M., E. Schwarzback (١٩٨٠) An inoculation technique for quantitative studies of brown rust resistance in barley
Barone A., E. Ritter, U. Schachtschabel, T. Debner, F. Salamini, C. Gebhardt (١٩٩٠) Localisation by restriction fragment length polymorphism mapping in Potato of a major dominant gene conferring resistance to the Potato Cyst nematode Globodera rostochiensis
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A half diallel set of crosses among six highly diverse barley (Hordeum
vulgare L.) genotypes were evaluated at Karahta station of field crops research
(GCSAR) Damascus Governorate, during 2010-2011 and 2011-2012 seasons to
study heterosis and comb
ining ability components for plant height, grain yield
per plant, number of spikes per plant, number of grains per spike and
thousand kernel weight.
This study present first estimates of segregation distortion
as revealed by comparison of segregation ratios in DH lines and sexually
produced F٢ lines in barley using the co-dominant microsatellite markers
(STMS).
The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
The sludge of wastewater used in agriculture processes, in order to knew the effect of
its application on soil and plant contents from zinc and cadmium, Hordeum vulgare L. was
planted by using graded amounts of sludge to the soil for two years, fou
r treatments were
used: o kg/pot (control), 2, 4, 6 ton/ha (with 5 erplicates for each treatment), concentrations
of zinc and cadmium in soil, roots, straw, and grain in all treatments were measured.
Results showed that the application of sludge improve the physical and chemical
properties of the soil, the concentration of zinc and cadmium in the soil were 164.66 μg/g,
324.22 ng/g, respectively, in 60 ton/ha treatment.
Concentration of Zn increased significantly and showed positive correlation with the
amount of sludge, the highest concentration of Zn was in root of plant in comparison with
other parts of plant, in all treatments, it was 69.3 μg/g in the root while it was 45.15, 38.2
μg/g in straw and grain respectively, in 0.6 kg/pot treatment.
Concentration of cadmium increased significantly in 0.6 kg/pot treatment in
comparison with control, the highest concentration was in the root of plant in comparison
with other parts of plant.
This study showed that Hordeum vulgare L. doesn't accumulate zinc and cadmium
because it´s concentration in roots was higher than in straw and grain.
Concentration of Zn and Cd still within permissible limits in the soil and plants in all
treatments.
This cultivation was carried out in the farm of the Faculty of Agriculture at Damascus University during the 2013-2014 agricultural season to study the effect of seeding rate per unit area and planting dates on yield and its components for three barley genotypes under rain-fed conditions.
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