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Register a new userThe effect of processing of pomegranate juice on antioxidant activity and its relationship with total phenols
تأثير معاملات تصنيع عصير الرمان في النشاط المضاد للأكسدة و علاقته بالفينولات
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Damascus University ورقة بحثية
Publication date
2014
fields
Food Sciences
and research's language is
العربية
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درس النشاط المضاد للأكسدة في عصير الرمان المستخلص بطريقتين و المعين بثلاث طرق، طريقة البيتاكاروتين، و مستحلب حمض لينولييك، و ثنائي فينيل بيكريل هيدرازيل. اعتمد في طريقة الاستخلاص الأولى للعصر على فرط حبات البذور يدوياً و عصرها باستخدام مكنة عصر نوع سيكلون (cyclone), (المعاملة- 1)، و اعتمدت في الطريقة الثانية على عصر أنصاف الثمار كاملة في نفس المكنة السابقة (المعاملة- 2). و استخلصت المركبات الفعالة من كلا نوعي العصير باستخدام الكحول الإيتيلي، و قورنت النتائج مع بوتيلات هيدروكسي أنيسول (BHA) كمضاد أكسدة مرجعي. أظهرت النتائج وجود فرق (0.05 > p) في النشاط المضاد للأكسدة بين نوعي عصير الرمان في المعاملات كافة. فبلغ معنوي %82.66 في المعاملة- 1 و 86.82 % في المعاملة- 2، و 86.98 % في BHA أظهر النشاط المضاد للأكسدة ارتباطاً معنوياً مع الفينولات الكلية في المعاملتين المدروستين في الطرق الثلاث لتعيين نشاط مضادات الأكسدة.
The antioxidant activity of pomegranate juices extracted by two methods was determined using three methods: β- carotene, Linoleic acid emulsion, and DiPhenel Picryl Hydrazyl (DPPH). The juice was extracted by separation arils from fruits and squeezed using Cyclone machine (T-1), while the second extraction method included squeezing the whole halve fruit with previous machine, (T-2). The potential compounds were determined using ethanol in a comparison with Butylated hydroxyl Anisole (BHA) as reference antioxidant. There were significant differences (P<0.05) in the antioxidant activity between the treatments in all measuring methods. The means of antioxidant activity amounted to 82.66% in T-1, 86.82% in T-2 and 86.98% in BHA. There was significant relationship between total phenols and antioxidant activity in all treatments.
References used
Asami, D., K. Hong. Y-J., Barrett, D., M. and A. E. Mitchell. 2004. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices, J. Agric. Food Chem. 51:1237-1241
Gil, M. I., F. A. Tomas-Barberan. B. Hess-Pierce. D. M. Holcroft and A. A. Kader. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing, J. Agric. Food Chem. 48: 4581- 4589
Hertog, M. G. L., P. Sweetnam. A. M. Fehily. P. C. Elwood and D. Kromhout. 1997. Antioxidant flavonols and ischemic heart disease in a welsh population of men. The Caerphilly study. Am. J.Clin .Nut ., 65 : 1489 - 1494
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In this research, the
antioxidant capacity(DPPH method), and total phenolic content (assayed
by Folin-Ciocalteu method), ,flavonoids, anthocyanins (pH differential
method) and ascorbic acids(2,6-dichlorophenol indophenol) in three fruit
tissues (
peel, pulp and whole fruit), of four apple cultivars commonly in
Syria(Red Delicious, Rome Beauty, Starking Delicious, Golden
Delicious), were studied.
This research studied the impact of planting location and cooking time
periods of (5, 10, 15, 20 and 40 minutes) on the antioxidant effect, and on the
total Phenols and Flavonoids contents using five different samples of Syrian
onions. The results
showed that the total Phenols, total Flavonoids and
antioxidant activity contents were between (279-583)mg/100g dry wt, (80-180)mg/100g dry wt, and (60-75%) respectively. In addition, boiling for
different periods resulted in increased total Phenols from (383 to789)mg/100g
dry wt and in total Flavonoids from (124 to 205)mg/100g dry wt as well as an
increase of (77% to 88%) of antioxidant activity as measured using the DPPH
method.
Classified locality Yeasts have been culturing in plantology
department at the Faculty of Science-University of Damascus,
from genera of Pichia, Candida, Geotrichum, Rhodotorula, Phaffia
on solid Czhapek agar medium, and after incubated in appropr
iate
conditions their colonies showed evidence of different colors to
produce a variety of pigments. Then it cultured in liquid Czhapek
medium to increase biomass and the genera producing
endocellular pigment separated from the medium by filtration with
added ethanol on them and blasting cells by ultrasonic waves to
get a pigment, while the genera producing exocellular pigment is
separated about the liquid medium also by filtration but the solvent
was added to the medium containing the pigment.
The changes in green peas, processed by using freezing and bleaching were
compared with their chemical composition (moisture, ash, sugar, fat). Effect of
freezing and bleaching processes of green peas on the ascorbic acid, total
phenolic compounds
,and antioxidant activity were investigated. Significant
changes were occurred between fresh and processed peas with regard to their
content of ascorbic acid, total phenols, and antioxidant activity as measured by
DPPH. Vitamin C content reduced to 65% in frozen peas and to16% in
bleaching peas. The decreased in total phenols was less, being 150mg/100g fw in
fresh peas reduced to 140 mg/100g fw in bleaching peas, and reached 120mg
/100g fw in freeze peas. These changes have been reflected significantly
(P<0.05) in antioxidant activities. Storage of green peas, frozen or boiled peas,
for 8 weeks, resulted in significant loss in vit C, antioxidant activity(P<0.05).
The main properties(yield of juice, total soluble solid, pH, total acidity, total
sugars), some antioxisant compounds as (vitamin C, carotenoids, total phenol,
anthocyanins), and antioxidant activity measured by DPPH method for some
main Syrian or
ange cultivars (navel, valancia, blood orange, and shamouti)
were investigated. The results showed that valancia was the highest in juice
yield (55.16%), while navel had the highest content of carotenoids(8.68mg/100g)
and the lowest one of vitamin C(31.53mg/100g). Whereas, blood orange was
superior in its content of total phenolic (295.73 mg gallic acid100/g) and
antioxidant activity, (74.833%) among the studied cultivars.
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