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Register a new userMonitoring the concentration of 17-β estradiol and progesterone with relative to puberty in growing Shami heifers
رصد مستوى هرموني االبروجِستيرون و الأستراديول و علاقته بالبلوغ في عجلات البقر الشامي
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Added by
Damascus University ورقة بحثية
Publication date
2014
fields
Animal Production
and research's language is
العربية
Created by
Shamra Editor
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استُخدمت 12 أنثى من عجلات البقر الشامي النامية في محطة بحوث دير الحجر لتحسين البقر الشامي متوسط أعمارها 178± 4 يوماً، و متوسط وزنها 115 ± 3 كغ لتقدير مستوى هرموني البروجِستيرون و الأستراديول في بلازما دم العجلات من عمر 6 و حتى 14 شهراً. سحبت عينات الدم أسبوعياً من الوريد الوداجي حيوانات التجربة كافة، و حفظت بلازما الدم على درجة حرارة - 20 س لحين إجراء التحليل. استخدمت طريقة المقايسة المناعية الإنزيمية (ELISA) لقياس مستوى هرموني الأستراديول و البروجِستيرون في الدم.
Twelve growing Shami heifers available in Dier-Al-Hajar Station for improving Shami Cattle weighing 115±3 kg and 178±4 days old were used to determine the weekly hormonal concentrations of progesterone (P4) and estradiol 17-β (E2) during the pre- and puberty stages (6-14 mos). Blood samples were collected weekly from the Jugular vein using vacutainer tubes supported with K3EDTA.
References used
Abdalla, K. F., S. M. Abdel-Aziz, A. F. El-Fakahany, A. S. El-Hamshary and L. M. Afifi. 1994. Effect of praziquantel on sex hormone levels in routine Schistosomiasis mansoni. J. Egy. Soc. Parasitol. 24: 627-632
Adams, G. P., R. L. Matteri, J. P. Kastelic, J. C. KO and O. J. Ginther. 1992. Association between surges of follicle-stimulating hormone and the emergence of follicular waves in heifers. J. Reprod. Fertil. 94: 177-188
Adashi, E. Y., C. E. Resnick, A. J. D'Ercole, M. E. Svoboda and J. J.Van Wyk. 1985. Insulin-like growth factors as intraovarian regulators of granulosa cell growth and function. Endocrinol. Rev. 6: 400-409
Artificial intelligence review:
Research summary
تتناول هذه الدراسة رصد مستويات هرموني البروجستيرون والأستراديول في بلازما دم عجلات البقر الشامي النامية وعلاقتها ببلوغها الجنسي. أجريت الدراسة على 12 عجلة في محطة بحوث دير الحجر، حيث تم جمع عينات الدم أسبوعياً من الوريد الوداجي وتحليلها باستخدام تقنية ELISA. أظهرت النتائج زيادة معنوية في تركيز هرمون الأستراديول مع تقدم العجلات في العمر واقترابها من سن البلوغ، حيث ارتفع متوسط تركيزه من 15.86 بغ/مل عند عمر 6 أشهر إلى 20.93 بغ/مل عند عمر 14 شهراً. في المقابل، لم يظهر هرمون البروجستيرون تغيرات معنوية في مستوياته خلال الفترة من 6 إلى 13 شهراً، لكنه ارتفع بشكل ملحوظ عند عمر 400 يوماً. استنتجت الدراسة أن عجلات البقر الشامي تصل إلى البلوغ الجنسي عند متوسط عمر 400 يوماً ووزن 250 كغ، وأوصت بضرورة البحث في أسباب ارتفاع مستوى هرمون الأستراديول في هذا النوع من الأبقار مقارنة بغيرها من السلالات.
Critical review
دراسة نقدية: تعتبر هذه الدراسة خطوة مهمة في فهم العوامل الهرمونية المؤثرة على بلوغ عجلات البقر الشامي. ومع ذلك، يمكن تحسين الدراسة من خلال زيادة حجم العينة لتكون أكثر تمثيلاً للسكان. كما أن الدراسة لم تتناول العوامل البيئية والغذائية بشكل كافٍ، والتي قد تؤثر على مستويات الهرمونات. بالإضافة إلى ذلك، كان من المفيد تضمين مقارنة مع سلالات أخرى تحت نفس الظروف البيئية والغذائية لتحديد ما إذا كانت الفروقات في مستويات الهرمونات ناتجة عن العوامل الوراثية أم البيئية.
Questions related to the research
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ما هو الهدف الرئيسي من الدراسة؟
الهدف الرئيسي هو رصد مستويات هرموني البروجستيرون والأستراديول في بلازما دم عجلات البقر الشامي النامية وتحديد علاقتها ببلوغها الجنسي.
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ما هي التقنية المستخدمة لقياس مستويات الهرمونات؟
تم استخدام تقنية المقايسة المناعية الإنزيمية (ELISA) لقياس مستويات هرموني الأستراديول والبروجستيرون.
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ما هي النتائج الرئيسية المتعلقة بهرمون الأستراديول؟
أظهرت النتائج زيادة معنوية في تركيز هرمون الأستراديول مع تقدم العجلات في العمر، حيث ارتفع متوسط تركيزه من 15.86 بغ/مل عند عمر 6 أشهر إلى 20.93 بغ/مل عند عمر 14 شهراً.
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ما هو الاستنتاج الرئيسي للدراسة؟
استنتجت الدراسة أن عجلات البقر الشامي تصل إلى البلوغ الجنسي عند متوسط عمر 400 يوماً ووزن 250 كغ، وأوصت بضرورة البحث في أسباب ارتفاع مستوى هرمون الأستراديول في هذا النوع من الأبقار مقارنة بغيرها من السلالات.
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Twelve growing Shami heifers available in Dier-Al-Hajar Station for
improving Shami Cattle weighing 115±3 kg and 178±4 days old were used to
monitor the daily growth of ovarian follicles (daily numbers, size, length and
number of waves, rate of gr
owth and regression) using Transrectal Ultrasound
Echo System (7.5 MHz probe) in 6-14 months-old heifers. Results indicated that
ovarian follicles developed in a wave pattern in all Shami heifers when they
were 6 months old and no significant differences in the number and size of
ovarian follicles were found between right and left ovaries. A cohort of 4 to 7
small follicles with a diameter 2-5 mm developed together and one of them was
selected for continual growth, became dominant and later regressed during the
pre-pubertal stage.
This study was carried out at the Shami Goat Improvement Station at
Karahta. Twenty one week old Shami male goats were used to measure the
concentrations of testosterone and LH hormones in plasma over a period of 21
months, using the Enzyme Linked
Immune Sorbent Assay (ELISA), in order to
determine the sexual puberty age. All kids were housed and managed under the
husbandry regimes applied at Karahta station.
Twelve growing Shami heifers available in Dier-Al-Hajar Station for
improving Shami Cattle during 2005-2007 weighing 250±11 kg and 400±21
days old were used to monitore the growth of dynamic of ovarian follicles (daily
numbers, size, length and nu
mber of waves, rate of growth and regression) and
characteristics of estrus cycle (length, time of dominancy, diameter of Graffian
follicle and estrus and ovulatory times), length of corpus luteum (CL) during
the estrous cycle. Heifers were maintained under uniform conditions and fed
according to their daily growth. Transrectal Ultrasound Echo System (7.5 MHz
probe) was used to study the ovarian structures and to monitor the daily
development and regression of ovarian follicles, the formation and regression of
corpus luteum in the left and right ovaries during the estrus cycle. Results
indicated that the growth turnover of ovarian follicles was observed and
developed in a wave pattern in all Shami heifers and no significant differences
in the number and sizes of ovarian follicles were found between right and left
ovaries. A cohort of 5 to 7 small follicles with a diameter 2-5 mm developed
together and one of them was selected to continue growing and becomes
dominant and later on being regressed or ovulated during the estrus cycle. One
to five follicular waves were observed per cycle and each wave was
characterized by the development of one large (dominant) follicle and a
variable number of smaller follicles. The estrus cycle with one follicular wave
occurred only in heifers exhibited the first post pubertal cycles with an average
length equaled to 8.67±0.33 days. Frequency of estrus cycle according to the
number of follicular waves was 8.4, 25.3, 44.2, 20 and 2.1% in cycles having 1,
2, 3, 4 and 5 follicular waves, respectively. Furthermore , the length of estrus
cycle was also related to the number of follicular waves and it was 21.87±0.62,
22.44±0.74, 26.4±0.72, 26.50±3.50 days in cycles having 2, 3, 4 and 5 follicular
waves, respectively with significant differences (P < 0.05) in cycles with 2 or 3
vs. 4 and/or 5 waves. The functional duration of CL was extended to 9, 12 and
15 days in a short (17-19 days), intermediate (20-24 days) and long estrus cycle
(≥ 25 days). The maximum diameter of dominant follicles in ovulatory waves
was significantly larger (P < 0.05) than that of dominant follicles in nonovulatory
waves. In conclusion, this study showed that the development of
ovarian follicles in Shami heifers occurs in waves and the most common pattern
is three waves per estrous cycle.
Ten growing Shami female dromedaries, 27 weeks old were used to
determine peripheral levels of glucose, IGF-1 and leptin in prepubertal Shami
dromedary heifers. Body weight(BW) was measured and blood samples were
collected weekly from the jugular
vein for 6 months in EDTA-containing tubes.
Serum was separated and stored at −20°C for later analysis. Glucose was
determined using a colorimetric glucose oxidase kit. Serum concentrations of
IGF-1 and leptin were assayed using some modifications of bovine radioimmunoassay
procedures. Animals were grouped according to their birth date,
body weight or average daily gain and variations in the plasma concentrations
of parameters as well as to the effect of studied factors were tested and assessed
by analysis of variance using the general linear model, repeated measurements
procedures of SAS. It is reported for the first time information on peripheral
levels of glucose, IGF-1 and leptin hormones in sexually immature dromedary
heifers and results indicated that BW had a significant effect (p<0.05) on IGF-1
levels. Overall means for glucose, Igf-1 and leptin, were 188 ± 4.11 mg\dl, 222 ±
6.43 ng\ml, and13.27 ± 0.16 ng\ml, respectively and it was found that those
concentrations were greater than values in sexually immature females in the
other domestic animals. Such information might be helpful for specialists to
search for the role of these hormones in the reproductive function and develop
breeding programs to reduce the period of the onset of puberty and increase
the reproductive performance of dromedary camels.
This study was carried out at Camel Research Station, Deer Al-Hajar,
Directorate of Animal Wealth Research, General Commission of Agricultural
Scientific Research to measure the testicular dimensions, TDs (Length, TL;
width, TW; Circumference, TC)
; the relationship between these parameters
and to use them as an indicator to determine the puberty age in Shami camel
males. Twenty five camel raised in the station were equally divided, according
to their age, into five groups: G1, almost one year,G2, G3, G4, and G5 were 2,
3, 4 and 5 years old, respectively.
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