Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userSexing of bovine Shami embryos using PCR
تجنيس أجنة الأبقار الشامية باستخدام تقانة التفاعل التسلسلي البوليميرازي
1604
0 51
0
(
0
)
Added by
Damascus University ورقة بحثية
Publication date
2013
fields
Animal Production
and research's language is
العربية
Created by
Shamra Editor
Ask ChatGPT about the research
استُخدم 13 جنيناً مجمداً بعد إذابتها بمرحلتي التويتة أو الكيسة الأريمية و ذات حيويـة جيـدة، حـصل عليها من أبقار شامية حية محرضة بطريقة الإباضة المتعددة و التلقيح الصنعي بهدف معرفة جنس الجنـين قبل نقله باستخدام تقانة التفاعل التسلسلي البـوليميرازي (PCR (Reaction Chain Polymerase. سحبت 1-2 قُسيم أرومي من كل منها باستخدام إبرة رفيعة مركبة على مجهر مقلوب. و استنتج أن سحب 2-1 قُسيم أرومي من أجنة الأبقار الشامية في مرحلة التويتة أو الكيسة الأريمية تكفي لتحديد جنس الجنين في الأبقار الشامية باستخدام تقانة التفاعل التسلسلي البوليميرازي المتعدد، و حددت، و لأول مرة بادئات نوعية يمكن استخدامها لتجنيس أجنة الأبقار الشامية، ما يفيد في تقنيات نقل الأجنة المرغوبة و تقسيمها و الإستفادة منها مبكراً في عمليات التحسين الوراثي.
Thirteen viable and post - frozenthawed Shami cows embryos obtained by super-ovulation and artificial insemination technique in order to determine sex of embryos. Biopsies (1-2 cells) of embryos at morula or blastocyst stage were taken using micromanipulation under inverted microscope. It was concluded that aspiration of 1-2 blastomere (s) from embryos at morula or blastocyst stage was enough to determine sex of embryo using PCR and it is recommended that using this technique can be a useful tool in splitting and transferring of desired sex embryos in genetic improvement program.
References used
Avery, B., A. Bak and M. Schmidt. 1989. Different cleavage rates and sex determination in bovine embryos. Theriogeno., 32: 139-147
Booman, P., L. Kruijt, R. Veerhuis, A. M. Hengst, M. Tiemann and F.E. Ruch.1989. Sexing bovine embryos with monoclonal antibodies against the H-Y antigen. Livestock Production Science, 23: 1–16
Cenariu, M., I. Groza, P. Emoke, L. Bogdan, I. Morar, S. Ciupe and R. Pop. 2011. Sexing of bovine embryos using polymerase chain reaction (PCR) and fluorescent In Situ hybridization (FISH). Romanian Biotechnological Letters, 16(2): 6055-6061
rate research
Read More
Twelve growing Shami heifers available in Dier-Al-Hajar Station for
improving Shami Cattle during 2005-2007 weighing 250±11 kg and 400±21
days old were used to monitore the growth of dynamic of ovarian follicles (daily
numbers, size, length and nu
mber of waves, rate of growth and regression) and
characteristics of estrus cycle (length, time of dominancy, diameter of Graffian
follicle and estrus and ovulatory times), length of corpus luteum (CL) during
the estrous cycle. Heifers were maintained under uniform conditions and fed
according to their daily growth. Transrectal Ultrasound Echo System (7.5 MHz
probe) was used to study the ovarian structures and to monitor the daily
development and regression of ovarian follicles, the formation and regression of
corpus luteum in the left and right ovaries during the estrus cycle. Results
indicated that the growth turnover of ovarian follicles was observed and
developed in a wave pattern in all Shami heifers and no significant differences
in the number and sizes of ovarian follicles were found between right and left
ovaries. A cohort of 5 to 7 small follicles with a diameter 2-5 mm developed
together and one of them was selected to continue growing and becomes
dominant and later on being regressed or ovulated during the estrus cycle. One
to five follicular waves were observed per cycle and each wave was
characterized by the development of one large (dominant) follicle and a
variable number of smaller follicles. The estrus cycle with one follicular wave
occurred only in heifers exhibited the first post pubertal cycles with an average
length equaled to 8.67±0.33 days. Frequency of estrus cycle according to the
number of follicular waves was 8.4, 25.3, 44.2, 20 and 2.1% in cycles having 1,
2, 3, 4 and 5 follicular waves, respectively. Furthermore , the length of estrus
cycle was also related to the number of follicular waves and it was 21.87±0.62,
22.44±0.74, 26.4±0.72, 26.50±3.50 days in cycles having 2, 3, 4 and 5 follicular
waves, respectively with significant differences (P < 0.05) in cycles with 2 or 3
vs. 4 and/or 5 waves. The functional duration of CL was extended to 9, 12 and
15 days in a short (17-19 days), intermediate (20-24 days) and long estrus cycle
(≥ 25 days). The maximum diameter of dominant follicles in ovulatory waves
was significantly larger (P < 0.05) than that of dominant follicles in nonovulatory
waves. In conclusion, this study showed that the development of
ovarian follicles in Shami heifers occurs in waves and the most common pattern
is three waves per estrous cycle.
This research was conducted I during 2009-2010 at Dier Alhajar station,
General Commission for Scientific Agricultural Research to determine the
length of calving interval (CI) and the effect of sire, calving year, calving
season, parity and age o
f cow and their interactions in Shami Cattle.
It was concluded that CI can be reduced by better
management and providing good and suitable feeding conditions.
This study was conducted at the biotechnology laboratory of AnimalWealth
Research Administration, General Commission for Agricultural Research in
order to compare the effect of different media on In vitro maturation (oocytes
matured at 39 C° under
5% CO2 for 24 h), fertilization، and culture (7 days
postfertilization) in humidified atmosphere 95% on bovine embryo production.
Syria
سورية
وسط الإنضاج
وسط تنمية الأجنة مخبرياً
وسط الإخصاب
عامل تهيئة النطاف في المختبر
إنتاج اجنة الأبقار مخبرياً
(Tissue culture medium (TCM
(Synthetic oviduct fluid (SOF
(Tyrode Albumin Lactate Pyruvate (TALP
(Penicillin Hypotaurin Epinephrine (PHE
bovine oocytes
In vitro embryo production
المزيد..
This study conducted at Deir Al-Hajar Station during the season 2010-2011
to describe the lactation curve of Shami cattle and determine factors affecting
its shape compenents. 1120 records of 356 Shami cows collected between 1997 –
2010 were used.
Data were subjected to general linear model, and the analysis
of variance used to determine the effect of factors. Duncan test was used to
compare the means using SAS system (1996) and Incomplete gamma functions
were used to estimate the parameters of lactation curve (a, b and on the basis of
daily yield of milk.
Results indicated that the average values of the gamma parameters were
2.14 ± 0.01 kg for a (beginning of milking ), 0.61 ± 0.02 kg for b (increasing milk
production up to peak) and - 0.23± 0.01 kg for c (decreasing milk production
from peak to dry). The effect of calving year was significant (p<0.01) on a, and
(p<0.05) on b and (p<0.001) on c. The effect of age at calving and sex of calf was
significant (p<0.01) on a but non significant on b and c. Season of calving,
parity, and interaction between age and parity was not significant on all
parameters.
It was concluded that improving the productive performance of Shami
cattle, requires applying a long term genetic improvement program to select the
best herd.
Experiment was conducted on 160 fertilized egg sterile, distributed
in four groups of 40 eggs in each of them, eggs incubation in the
hatchery for 12 h, and then go out and inject eggs experimental
Groups (1-2-3) with a solution of tetracycline under the embryo disk.
suggested questions
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
