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Register a new userPartial purification of hydroxycinnamic acid esterase and study of its activity in the synthesis of ferulic acid ethyl ester
التنقية الجزئية لاستيراز حمض هيدروكسي السيناميك و دراسة فعاليته في اصطناع الإستر الإيتيلي لحمض الفيروليك
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جرت تنقية جزئية لاستيراز حمض هيدروكسي السيناميك فطري المنشأ من Humicola sp. و ذلك بترسيبه إما باستخدام سلفات الأمونيوم أو الأسيتون.
A partial purification was performed for the fungal hydroxycinnamic acid esterase (HCAE) from Humicola sp. by precipitation using either ammonium sulfate or acetone.
References used
Ralet M. C., Thibault J. F., Faulds C. B., Williamson G. (1994). Isolation and purification of feruloylated oligosaccharides from cell-walls of sugar-beet pulp. Carbohydrate Research, V. 263, pp. 227-241
Ishii T. (1997). Structure and functions of feruloylated polysaccharides. Plant Science, V. 127, pp. 111-127
Thibault J. F., Asther M., Ceccaldi B. C., Couteau D., Delattre M., Duarte J.C., Faulds C., Heldt-Hansen H., Kroon P., Lesage-Meessen L., Micard V., Renard C., Tuohy M., Hulle S. V., Williamson G. (1998). Fungal Bioconversion of Agricultural By-Products to Vanillin. Lebensmittel- Wissenschaft und Technologie, V. 31, pp. 530-536
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This study evaluated the in vitro
antileishmanial activity of some Phenolic compounds including:
Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid
against leishmanial tropica promastigotes. The parasite viability
was determined using XTT proliferation Kit.
In this article, a polyester amide was synthesized from oleic acid. This polymer is a biodegradable and thermoplastic polymer, with wide medical and biological applications due to its being compounds with a linear structure. The polymer structure has
been confirmed. Output using spectroscopy methods known as 1H-NMR, and FT-IR spectroscopy. And we obtained this polymer with a good yield (78%).
The 1,3-dipolar cycloaddition of the aryl azide and some its of derivatives with cinnamic
acid was studied theoretically using different methods of Density Functional Theory
(DFT): B3LYP, B3PW91, MPW1PBE, MPW1PW91 and M062X with the basic set 6-
3
1g(d). Most of the used levels of theories provide closed results qualitatively, and the
results of the level of theory M062X / 6-31g (d) show that these reactions in the gas state
carried out spontaneously to form several stable energy products; the products P1 and P2
are most stable, but all the reactions are very slow and one of the products P1 is more
spontaneous than P2 , but the formation reaction of P2 is faster.
On the other hand, when these reactions were studied with different solvents, it is found
that solvents to have no actual effects on the rates of these reactions.
Hetrocyclic compounds 5-(5-R-2-Furfurlidene)– barbituric acid were
obtained and their physical and chemical properties were studied. Their
structures were identified by spectoroscopic methods. This study proved by
1H-NMR Spectroscopy data that these compounds exist in S-cis form.
Sugar fatty acid esters are nonionic surfactants, which are used for personal
care products, cosmetic applications, and as emulsifiers for food. In recent
years, enzymatic synthesis of sugar fatty acid esters is attracting keen attention
as a new
manufacturing method for future application. Some Reaction
parameters of lipase-catalyzed synthesis of SFAE in organic solvent were
optimized in a batch reactor. Optimum conditions for reaction performed were
10% (w/w of substrates) of lipase from Candida antarctica B, at 60°C. Among
the solvents tested in this study, the highest yield (93%) was obtained when
Aceton was used as a solvent,. The optimal sugar-to-fatty acid ratio was tested
in the range from 3:1 to 1:7 and verious sugars were used. A high conversion
was obtained when the ratio of Sugar: Fatty acid (1:5) and Froctuse were used
as substrat, respectively.
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