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In vivo Study of the effect of seeds extraction of Anise plant in the growth of the two pathogenic fungi Fusarium oxysporum and Aspergillus niger

تأثير المستخلصات الخلوية لبذور اليانسون في نمو الفطرين الممرضين Fusarium oxysporum و Aspergillus niger

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 Publication date 2017
  fields Life Science
and research's language is العربية
 Created by Shamra Editor




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A Study was carried out to evaluate the ability of the methanolic, hydro and oily extractions of anise seeds (Pimpinella anisum L.) to inhibit the growth of the pathogenic fungi Fusarium oxysporum and Aspergillus niger. The results showed a good inhibition ability of the alcoholic and hydro extractions on the growth of the studied fungi. The inhibition ability differed due to the used concentrations. When the methanolic extraction was used by concentrations (2, 4, and 6) mlg/ ml at the seventh day, the inhibition ability was (42.5, 50.6 and 64.4)% of the fungus F. oxysporum, respectively. And was (23.6, 42.7 and 55.1)% of the fungus A. niger, respectively. Whereas when the hydro extraction was used by the same concentrations, the inhibition ability was (59.2, 65.3 and 70)% respectively for the fungus F. oxysporum, and (46.1, 58 and 61)% for the fungus A. niger, respectively. The oily extraction showed high inhibition ability, where the inhibition rate was 100% when the extraction was used by the two concentrations 50 and 75 μl for both two studied fungi.


Artificial intelligence review:
Research summary
تناول البحث دراسة تأثير مستخلصات بذور نبات اليانسون (Pimpinella anisum L.) على نمو الفطرين الممرضين Aspergillus niger و Fusarium oxysporum. تم استخدام مستخلصات ميثانولية ومائية وزيتية بتركيزات مختلفة لتحديد قدرتها على تثبيط نمو الفطريات. أظهرت النتائج أن جميع المستخلصات كانت فعالة في تثبيط نمو الفطريات، حيث كانت الفعالية التثبيطية تعتمد على تركيز المستخلص ونوع المذيب. المستخلص الميثانولي بتركيزات (2، 4، و6) ملغ/مل أظهر نسب تثبيط تتراوح بين 42.5% و64.4% للفطر F. oxysporum وبين 23.6% و55.1% للفطر A. niger. أما المستخلص المائي، فقد أظهر نسب تثبيط تتراوح بين 59.2% و70% للفطر F. oxysporum وبين 46.1% و61% للفطر A. niger. المستخلص الزيتي أظهر أعلى نسبة تثبيط بلغت 100% عند استخدام تركيزات 50 و75 ميكرولتر لكلا الفطرين. تشير النتائج إلى أن مستخلصات بذور اليانسون تمتلك قدرة تثبيطية عالية تجاه الفطريات الممرضة، مما يجعلها واعدة في مجال مكافحة الفطريات باستخدام مواد ذات منشأ حيوي فعالة دون الضرر بالبيئة.
Critical review
دراسة نقدية: يعتبر هذا البحث خطوة هامة نحو استخدام المستخلصات النباتية كبديل طبيعي للمبيدات الكيميائية في مكافحة الفطريات الممرضة. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل توسيع نطاق الدراسة لتشمل أنواعاً أخرى من الفطريات الممرضة لتعميم النتائج. ثانياً، لم يتم التطرق بشكل كافٍ إلى الآليات الكيميائية التي تجعل مستخلصات اليانسون فعالة ضد الفطريات، مما يترك مجالاً للتساؤل حول كيفية عمل هذه المستخلصات على المستوى الجزيئي. أخيراً، كان من الممكن تضمين تجارب ميدانية لتأكيد فعالية المستخلصات في ظروف الزراعة الحقيقية، وليس فقط في المختبر.
Questions related to the research
  1. ما هي المستخلصات التي تم استخدامها في الدراسة؟

    تم استخدام مستخلصات ميثانولية ومائية وزيتية لبذور اليانسون.

  2. ما هي الفطريات التي تم اختبارها في الدراسة؟

    تم اختبار الفطريات Aspergillus niger و Fusarium oxysporum.

  3. ما هي أعلى نسبة تثبيط تم تحقيقها في الدراسة؟

    أعلى نسبة تثبيط تم تحقيقها كانت 100% باستخدام المستخلص الزيتي بتركيزات 50 و75 ميكرولتر.

  4. هل كانت الفعالية التثبيطية تعتمد على تركيز المستخلص؟

    نعم، كانت الفعالية التثبيطية تعتمد على تركيز المستخلص ونوع المذيب المستخدم.


References used
Abd El-Ghany T. M, Roushdy M. M, Mohamed A. 2015 Efficacy of Certain Plant Extracts as Safe Fungicides Against Phytopathogenic and Mycotoxigenic Fungi. Agricultural and Biological Sciences Journal .Vol. 1, No. 3 , pp. 71-75
Agrios, G.N. 2005. Plant Diseases Caused By Fungi. In Plant Pathology, 5th ed.; Agrios, G.N., Ed.; Academic Press: San Diego, CA, USA,; Chapter Eleven; pp. 385–614
Al-Rahmah A. N, Mostafa A. A, Abdel-Megeed A, Yakout S. M, and Hussein S. A. 2013 Fungicidal activities of certain methanolic plant extracts against tomato phytopathogenic fungi. African Journal of Microbiology Research Vol. 7(6), pp. 517-524
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